Cargando…
Functionally distinct BMP1 isoforms show an opposite pattern of abundance in plasma from non-small cell lung cancer subjects and controls
Advancements in deep plasma proteomics are enabling high-resolution measurement of plasma proteoforms, which may reveal a rich source of novel biomarkers previously concealed by aggregated protein methods. Here, we analyze 188 plasma proteomes from non-small cell lung cancer subjects (NSCLC) and con...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058078/ https://www.ncbi.nlm.nih.gov/pubmed/36989217 http://dx.doi.org/10.1371/journal.pone.0282821 |
_version_ | 1785016530193350656 |
---|---|
author | Donovan, Margaret K. R. Huang, Yingxiang Blume, John E. Wang, Jian Hornburg, Daniel Ferdosi, Shadi Mohtashemi, Iman Kim, Sangtae Ko, Marwin Benz, Ryan W. Platt, Theodore L. Batzoglou, Serafim Diaz, Luis A. Farokhzad, Omid C. Siddiqui, Asim |
author_facet | Donovan, Margaret K. R. Huang, Yingxiang Blume, John E. Wang, Jian Hornburg, Daniel Ferdosi, Shadi Mohtashemi, Iman Kim, Sangtae Ko, Marwin Benz, Ryan W. Platt, Theodore L. Batzoglou, Serafim Diaz, Luis A. Farokhzad, Omid C. Siddiqui, Asim |
author_sort | Donovan, Margaret K. R. |
collection | PubMed |
description | Advancements in deep plasma proteomics are enabling high-resolution measurement of plasma proteoforms, which may reveal a rich source of novel biomarkers previously concealed by aggregated protein methods. Here, we analyze 188 plasma proteomes from non-small cell lung cancer subjects (NSCLC) and controls to identify NSCLC-associated protein isoforms by examining differentially abundant peptides as a proxy for isoform-specific exon usage. We find four proteins comprised of peptides with opposite patterns of abundance between cancer and control subjects. One of these proteins, BMP1, has known isoforms that can explain this differential pattern, for which the abundance of the NSCLC-associated isoform increases with stage of NSCLC progression. The presence of cancer and control-associated isoforms suggests differential regulation of BMP1 isoforms. The identified BMP1 isoforms have known functional differences, which may reveal insights into mechanisms impacting NSCLC disease progression. |
format | Online Article Text |
id | pubmed-10058078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-100580782023-03-30 Functionally distinct BMP1 isoforms show an opposite pattern of abundance in plasma from non-small cell lung cancer subjects and controls Donovan, Margaret K. R. Huang, Yingxiang Blume, John E. Wang, Jian Hornburg, Daniel Ferdosi, Shadi Mohtashemi, Iman Kim, Sangtae Ko, Marwin Benz, Ryan W. Platt, Theodore L. Batzoglou, Serafim Diaz, Luis A. Farokhzad, Omid C. Siddiqui, Asim PLoS One Research Article Advancements in deep plasma proteomics are enabling high-resolution measurement of plasma proteoforms, which may reveal a rich source of novel biomarkers previously concealed by aggregated protein methods. Here, we analyze 188 plasma proteomes from non-small cell lung cancer subjects (NSCLC) and controls to identify NSCLC-associated protein isoforms by examining differentially abundant peptides as a proxy for isoform-specific exon usage. We find four proteins comprised of peptides with opposite patterns of abundance between cancer and control subjects. One of these proteins, BMP1, has known isoforms that can explain this differential pattern, for which the abundance of the NSCLC-associated isoform increases with stage of NSCLC progression. The presence of cancer and control-associated isoforms suggests differential regulation of BMP1 isoforms. The identified BMP1 isoforms have known functional differences, which may reveal insights into mechanisms impacting NSCLC disease progression. Public Library of Science 2023-03-29 /pmc/articles/PMC10058078/ /pubmed/36989217 http://dx.doi.org/10.1371/journal.pone.0282821 Text en © 2023 Donovan et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Donovan, Margaret K. R. Huang, Yingxiang Blume, John E. Wang, Jian Hornburg, Daniel Ferdosi, Shadi Mohtashemi, Iman Kim, Sangtae Ko, Marwin Benz, Ryan W. Platt, Theodore L. Batzoglou, Serafim Diaz, Luis A. Farokhzad, Omid C. Siddiqui, Asim Functionally distinct BMP1 isoforms show an opposite pattern of abundance in plasma from non-small cell lung cancer subjects and controls |
title | Functionally distinct BMP1 isoforms show an opposite pattern of abundance in plasma from non-small cell lung cancer subjects and controls |
title_full | Functionally distinct BMP1 isoforms show an opposite pattern of abundance in plasma from non-small cell lung cancer subjects and controls |
title_fullStr | Functionally distinct BMP1 isoforms show an opposite pattern of abundance in plasma from non-small cell lung cancer subjects and controls |
title_full_unstemmed | Functionally distinct BMP1 isoforms show an opposite pattern of abundance in plasma from non-small cell lung cancer subjects and controls |
title_short | Functionally distinct BMP1 isoforms show an opposite pattern of abundance in plasma from non-small cell lung cancer subjects and controls |
title_sort | functionally distinct bmp1 isoforms show an opposite pattern of abundance in plasma from non-small cell lung cancer subjects and controls |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058078/ https://www.ncbi.nlm.nih.gov/pubmed/36989217 http://dx.doi.org/10.1371/journal.pone.0282821 |
work_keys_str_mv | AT donovanmargaretkr functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT huangyingxiang functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT blumejohne functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT wangjian functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT hornburgdaniel functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT ferdosishadi functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT mohtashemiiman functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT kimsangtae functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT komarwin functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT benzryanw functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT platttheodorel functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT batzoglouserafim functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT diazluisa functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT farokhzadomidc functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols AT siddiquiasim functionallydistinctbmp1isoformsshowanoppositepatternofabundanceinplasmafromnonsmallcelllungcancersubjectsandcontrols |