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Transcriptomic Study on Human Skin Samples: Identification of Two Subclasses of Actinic Keratoses

Actinic keratoses (AKs) are sun-damaged skin areas that affect 20% of the European adult population and more than 50% of people aged 70 years and over. There are currently no clinical or histological features allowing us to identify to which clinical class (i.e., regression or progression) an AK bel...

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Autores principales: Dubois-Pot-Schneider, Hélène, Khairallah, Grégoire, Brzenczek, Cyril, Plénat, François, Marchal, Frédéric, Amouroux, Marine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058209/
https://www.ncbi.nlm.nih.gov/pubmed/36983009
http://dx.doi.org/10.3390/ijms24065937
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author Dubois-Pot-Schneider, Hélène
Khairallah, Grégoire
Brzenczek, Cyril
Plénat, François
Marchal, Frédéric
Amouroux, Marine
author_facet Dubois-Pot-Schneider, Hélène
Khairallah, Grégoire
Brzenczek, Cyril
Plénat, François
Marchal, Frédéric
Amouroux, Marine
author_sort Dubois-Pot-Schneider, Hélène
collection PubMed
description Actinic keratoses (AKs) are sun-damaged skin areas that affect 20% of the European adult population and more than 50% of people aged 70 years and over. There are currently no clinical or histological features allowing us to identify to which clinical class (i.e., regression or progression) an AK belongs. A transcriptomic approach seems to be a robust tool for AK characterization, but there is a need for additional studies, including more patients and elucidating the molecular signature of an AK. In this context, the present study, including the largest number of patients to date, is the first aiming at identifying biological features to objectively distinguish different AK signatures. We highlight two distinct molecular profiles: AKs featuring a molecular profile similar to squamous cell carcinomas (SCCs), which are called “lesional AKs” (AK_Ls), and AKs featuring a molecular profile similar to normal skin tissue, which are called “non-lesional AKs” (AK_NLs). The molecular profiles of both AK subclasses were studied, and 316 differentially expressed genes (DEGs) were identified between the two classes. The 103 upregulated genes in AK_L were related to the inflammatory response. Interestingly, downregulated genes were associated with keratinization. Finally, based on a connectivity map approach, our data highlight that the VEGF pathway could be a promising therapeutic target for high-risk lesions.
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spelling pubmed-100582092023-03-30 Transcriptomic Study on Human Skin Samples: Identification of Two Subclasses of Actinic Keratoses Dubois-Pot-Schneider, Hélène Khairallah, Grégoire Brzenczek, Cyril Plénat, François Marchal, Frédéric Amouroux, Marine Int J Mol Sci Article Actinic keratoses (AKs) are sun-damaged skin areas that affect 20% of the European adult population and more than 50% of people aged 70 years and over. There are currently no clinical or histological features allowing us to identify to which clinical class (i.e., regression or progression) an AK belongs. A transcriptomic approach seems to be a robust tool for AK characterization, but there is a need for additional studies, including more patients and elucidating the molecular signature of an AK. In this context, the present study, including the largest number of patients to date, is the first aiming at identifying biological features to objectively distinguish different AK signatures. We highlight two distinct molecular profiles: AKs featuring a molecular profile similar to squamous cell carcinomas (SCCs), which are called “lesional AKs” (AK_Ls), and AKs featuring a molecular profile similar to normal skin tissue, which are called “non-lesional AKs” (AK_NLs). The molecular profiles of both AK subclasses were studied, and 316 differentially expressed genes (DEGs) were identified between the two classes. The 103 upregulated genes in AK_L were related to the inflammatory response. Interestingly, downregulated genes were associated with keratinization. Finally, based on a connectivity map approach, our data highlight that the VEGF pathway could be a promising therapeutic target for high-risk lesions. MDPI 2023-03-21 /pmc/articles/PMC10058209/ /pubmed/36983009 http://dx.doi.org/10.3390/ijms24065937 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dubois-Pot-Schneider, Hélène
Khairallah, Grégoire
Brzenczek, Cyril
Plénat, François
Marchal, Frédéric
Amouroux, Marine
Transcriptomic Study on Human Skin Samples: Identification of Two Subclasses of Actinic Keratoses
title Transcriptomic Study on Human Skin Samples: Identification of Two Subclasses of Actinic Keratoses
title_full Transcriptomic Study on Human Skin Samples: Identification of Two Subclasses of Actinic Keratoses
title_fullStr Transcriptomic Study on Human Skin Samples: Identification of Two Subclasses of Actinic Keratoses
title_full_unstemmed Transcriptomic Study on Human Skin Samples: Identification of Two Subclasses of Actinic Keratoses
title_short Transcriptomic Study on Human Skin Samples: Identification of Two Subclasses of Actinic Keratoses
title_sort transcriptomic study on human skin samples: identification of two subclasses of actinic keratoses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058209/
https://www.ncbi.nlm.nih.gov/pubmed/36983009
http://dx.doi.org/10.3390/ijms24065937
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