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A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics
We introduce a cost-effective, robust high-throughput–compatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day. The method has been fully validated by processing >3000 samples with no apparent batch eff...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Association for the Advancement of Science
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058233/ https://www.ncbi.nlm.nih.gov/pubmed/36989362 http://dx.doi.org/10.1126/sciadv.adf9717 |
| _version_ | 1785016576034996224 |
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| author | Viode, Arthur van Zalm, Patrick Smolen, Kinga K. Fatou, Benoit Stevenson, David Jha, Meenakshi Levy, Ofer Steen, Judith Steen, Hanno |
| author_facet | Viode, Arthur van Zalm, Patrick Smolen, Kinga K. Fatou, Benoit Stevenson, David Jha, Meenakshi Levy, Ofer Steen, Judith Steen, Hanno |
| author_sort | Viode, Arthur |
| collection | PubMed |
| description | We introduce a cost-effective, robust high-throughput–compatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day. The method has been fully validated by processing >3000 samples with no apparent batch effect at a cost for the depletion step of ~$2.5 per sample. |
| format | Online Article Text |
| id | pubmed-10058233 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | American Association for the Advancement of Science |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-100582332023-03-30 A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics Viode, Arthur van Zalm, Patrick Smolen, Kinga K. Fatou, Benoit Stevenson, David Jha, Meenakshi Levy, Ofer Steen, Judith Steen, Hanno Sci Adv Biomedicine and Life Sciences We introduce a cost-effective, robust high-throughput–compatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day. The method has been fully validated by processing >3000 samples with no apparent batch effect at a cost for the depletion step of ~$2.5 per sample. American Association for the Advancement of Science 2023-03-29 /pmc/articles/PMC10058233/ /pubmed/36989362 http://dx.doi.org/10.1126/sciadv.adf9717 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Biomedicine and Life Sciences Viode, Arthur van Zalm, Patrick Smolen, Kinga K. Fatou, Benoit Stevenson, David Jha, Meenakshi Levy, Ofer Steen, Judith Steen, Hanno A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics |
| title | A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics |
| title_full | A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics |
| title_fullStr | A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics |
| title_full_unstemmed | A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics |
| title_short | A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics |
| title_sort | simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics |
| topic | Biomedicine and Life Sciences |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058233/ https://www.ncbi.nlm.nih.gov/pubmed/36989362 http://dx.doi.org/10.1126/sciadv.adf9717 |
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