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How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth

Controlled tissue growth is essential for multicellular life and requires tight spatiotemporal control over cell proliferation and differentiation until reaching homeostasis. As cells synthesize and remodel extracellular matrix, tissue growth processes can only be understood if the reciprocal feedba...

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Autores principales: Benn, Mario C., Pot, Simon A., Moeller, Jens, Yamashita, Tadahiro, Fonta, Charlotte M., Orend, Gertraud, Kollmannsberger, Philip, Vogel, Viola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058249/
https://www.ncbi.nlm.nih.gov/pubmed/36989370
http://dx.doi.org/10.1126/sciadv.add9275
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author Benn, Mario C.
Pot, Simon A.
Moeller, Jens
Yamashita, Tadahiro
Fonta, Charlotte M.
Orend, Gertraud
Kollmannsberger, Philip
Vogel, Viola
author_facet Benn, Mario C.
Pot, Simon A.
Moeller, Jens
Yamashita, Tadahiro
Fonta, Charlotte M.
Orend, Gertraud
Kollmannsberger, Philip
Vogel, Viola
author_sort Benn, Mario C.
collection PubMed
description Controlled tissue growth is essential for multicellular life and requires tight spatiotemporal control over cell proliferation and differentiation until reaching homeostasis. As cells synthesize and remodel extracellular matrix, tissue growth processes can only be understood if the reciprocal feedback between cells and their environment is revealed. Using de novo–grown microtissues, we identified crucial actors of the mechanoregulated events, which iteratively orchestrate a sharp transition from tissue growth to maturation, requiring a myofibroblast-to-fibroblast transition. Cellular decision-making occurs when fibronectin fiber tension switches from highly stretched to relaxed, and it requires the transiently up-regulated appearance of tenascin-C and tissue transglutaminase, matrix metalloprotease activity, as well as a switch from α5β1 to α2β1 integrin engagement and epidermal growth factor receptor signaling. As myofibroblasts are associated with wound healing and inflammatory or fibrotic diseases, crucial knowledge needed to advance regenerative strategies or to counter fibrosis and cancer progression has been gained.
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spelling pubmed-100582492023-03-30 How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth Benn, Mario C. Pot, Simon A. Moeller, Jens Yamashita, Tadahiro Fonta, Charlotte M. Orend, Gertraud Kollmannsberger, Philip Vogel, Viola Sci Adv Biomedicine and Life Sciences Controlled tissue growth is essential for multicellular life and requires tight spatiotemporal control over cell proliferation and differentiation until reaching homeostasis. As cells synthesize and remodel extracellular matrix, tissue growth processes can only be understood if the reciprocal feedback between cells and their environment is revealed. Using de novo–grown microtissues, we identified crucial actors of the mechanoregulated events, which iteratively orchestrate a sharp transition from tissue growth to maturation, requiring a myofibroblast-to-fibroblast transition. Cellular decision-making occurs when fibronectin fiber tension switches from highly stretched to relaxed, and it requires the transiently up-regulated appearance of tenascin-C and tissue transglutaminase, matrix metalloprotease activity, as well as a switch from α5β1 to α2β1 integrin engagement and epidermal growth factor receptor signaling. As myofibroblasts are associated with wound healing and inflammatory or fibrotic diseases, crucial knowledge needed to advance regenerative strategies or to counter fibrosis and cancer progression has been gained. American Association for the Advancement of Science 2023-03-29 /pmc/articles/PMC10058249/ /pubmed/36989370 http://dx.doi.org/10.1126/sciadv.add9275 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Benn, Mario C.
Pot, Simon A.
Moeller, Jens
Yamashita, Tadahiro
Fonta, Charlotte M.
Orend, Gertraud
Kollmannsberger, Philip
Vogel, Viola
How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth
title How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth
title_full How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth
title_fullStr How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth
title_full_unstemmed How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth
title_short How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth
title_sort how the mechanobiology orchestrates the iterative and reciprocal ecm-cell cross-talk that drives microtissue growth
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058249/
https://www.ncbi.nlm.nih.gov/pubmed/36989370
http://dx.doi.org/10.1126/sciadv.add9275
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