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How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth
Controlled tissue growth is essential for multicellular life and requires tight spatiotemporal control over cell proliferation and differentiation until reaching homeostasis. As cells synthesize and remodel extracellular matrix, tissue growth processes can only be understood if the reciprocal feedba...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058249/ https://www.ncbi.nlm.nih.gov/pubmed/36989370 http://dx.doi.org/10.1126/sciadv.add9275 |
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author | Benn, Mario C. Pot, Simon A. Moeller, Jens Yamashita, Tadahiro Fonta, Charlotte M. Orend, Gertraud Kollmannsberger, Philip Vogel, Viola |
author_facet | Benn, Mario C. Pot, Simon A. Moeller, Jens Yamashita, Tadahiro Fonta, Charlotte M. Orend, Gertraud Kollmannsberger, Philip Vogel, Viola |
author_sort | Benn, Mario C. |
collection | PubMed |
description | Controlled tissue growth is essential for multicellular life and requires tight spatiotemporal control over cell proliferation and differentiation until reaching homeostasis. As cells synthesize and remodel extracellular matrix, tissue growth processes can only be understood if the reciprocal feedback between cells and their environment is revealed. Using de novo–grown microtissues, we identified crucial actors of the mechanoregulated events, which iteratively orchestrate a sharp transition from tissue growth to maturation, requiring a myofibroblast-to-fibroblast transition. Cellular decision-making occurs when fibronectin fiber tension switches from highly stretched to relaxed, and it requires the transiently up-regulated appearance of tenascin-C and tissue transglutaminase, matrix metalloprotease activity, as well as a switch from α5β1 to α2β1 integrin engagement and epidermal growth factor receptor signaling. As myofibroblasts are associated with wound healing and inflammatory or fibrotic diseases, crucial knowledge needed to advance regenerative strategies or to counter fibrosis and cancer progression has been gained. |
format | Online Article Text |
id | pubmed-10058249 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-100582492023-03-30 How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth Benn, Mario C. Pot, Simon A. Moeller, Jens Yamashita, Tadahiro Fonta, Charlotte M. Orend, Gertraud Kollmannsberger, Philip Vogel, Viola Sci Adv Biomedicine and Life Sciences Controlled tissue growth is essential for multicellular life and requires tight spatiotemporal control over cell proliferation and differentiation until reaching homeostasis. As cells synthesize and remodel extracellular matrix, tissue growth processes can only be understood if the reciprocal feedback between cells and their environment is revealed. Using de novo–grown microtissues, we identified crucial actors of the mechanoregulated events, which iteratively orchestrate a sharp transition from tissue growth to maturation, requiring a myofibroblast-to-fibroblast transition. Cellular decision-making occurs when fibronectin fiber tension switches from highly stretched to relaxed, and it requires the transiently up-regulated appearance of tenascin-C and tissue transglutaminase, matrix metalloprotease activity, as well as a switch from α5β1 to α2β1 integrin engagement and epidermal growth factor receptor signaling. As myofibroblasts are associated with wound healing and inflammatory or fibrotic diseases, crucial knowledge needed to advance regenerative strategies or to counter fibrosis and cancer progression has been gained. American Association for the Advancement of Science 2023-03-29 /pmc/articles/PMC10058249/ /pubmed/36989370 http://dx.doi.org/10.1126/sciadv.add9275 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Benn, Mario C. Pot, Simon A. Moeller, Jens Yamashita, Tadahiro Fonta, Charlotte M. Orend, Gertraud Kollmannsberger, Philip Vogel, Viola How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth |
title | How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth |
title_full | How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth |
title_fullStr | How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth |
title_full_unstemmed | How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth |
title_short | How the mechanobiology orchestrates the iterative and reciprocal ECM-cell cross-talk that drives microtissue growth |
title_sort | how the mechanobiology orchestrates the iterative and reciprocal ecm-cell cross-talk that drives microtissue growth |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058249/ https://www.ncbi.nlm.nih.gov/pubmed/36989370 http://dx.doi.org/10.1126/sciadv.add9275 |
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