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Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles

Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC) are receiving increasing interest as an approach to encapsulate natural extracts to increase the physicochemical stability of bioactives. Cannabis extract-derived cannabidiol (CBD) has potent therapeutic properties, including an...

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Autores principales: Zielińska, Aleksandra, da Ana, Raquel, Fonseca, Joel, Szalata, Milena, Wielgus, Karolina, Fathi, Faezeh, Oliveira, M. Beatriz P. P., Staszewski, Rafał, Karczewski, Jacek, Souto, Eliana B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058297/
https://www.ncbi.nlm.nih.gov/pubmed/36985847
http://dx.doi.org/10.3390/molecules28062875
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author Zielińska, Aleksandra
da Ana, Raquel
Fonseca, Joel
Szalata, Milena
Wielgus, Karolina
Fathi, Faezeh
Oliveira, M. Beatriz P. P.
Staszewski, Rafał
Karczewski, Jacek
Souto, Eliana B.
author_facet Zielińska, Aleksandra
da Ana, Raquel
Fonseca, Joel
Szalata, Milena
Wielgus, Karolina
Fathi, Faezeh
Oliveira, M. Beatriz P. P.
Staszewski, Rafał
Karczewski, Jacek
Souto, Eliana B.
author_sort Zielińska, Aleksandra
collection PubMed
description Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC) are receiving increasing interest as an approach to encapsulate natural extracts to increase the physicochemical stability of bioactives. Cannabis extract-derived cannabidiol (CBD) has potent therapeutic properties, including anti-inflammatory, antioxidant, and neuroprotective properties. In this work, physicochemical characterization was carried out after producing Compritol-based nanoparticles (cSLN or cNLC) loaded with CBD. Then, the determination of the encapsulation efficiency (EE), loading capacity (LC), particle size (Z-Ave), polydispersity index (PDI), and zeta potential were performed. Additionally, the viscoelastic profiles and differential scanning calorimetry (DSC) patterns were recorded. As a result, CBD-loaded SLN showed a mean particle size of 217.2 ± 6.5 nm, PDI of 0.273 ± 0.023, and EE of about 74%, while CBD-loaded NLC showed Z-Ave of 158.3 ± 6.6 nm, PDI of 0.325 ± 0.016, and EE of about 70%. The rheological analysis showed that the loss modulus for both lipid nanoparticle formulations was higher than the storage modulus over the applied frequency range of 10 Hz, demonstrating that they are more elastic than viscous. The crystallinity profiles of both CBD-cSLN (90.41%) and CBD-cNLC (40.18%) were determined. It may justify the obtained encapsulation parameters while corroborating the liquid-like character demonstrated in the rheological analysis. Scanning electron microscopy (SEM) study confirmed the morphology and shape of the developed nanoparticles. The work has proven that the solid nature and morphology of cSLN/cNLC strengthen these particles’ potential to modify the CBD delivery profile for several biomedical applications.
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spelling pubmed-100582972023-03-30 Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles Zielińska, Aleksandra da Ana, Raquel Fonseca, Joel Szalata, Milena Wielgus, Karolina Fathi, Faezeh Oliveira, M. Beatriz P. P. Staszewski, Rafał Karczewski, Jacek Souto, Eliana B. Molecules Article Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC) are receiving increasing interest as an approach to encapsulate natural extracts to increase the physicochemical stability of bioactives. Cannabis extract-derived cannabidiol (CBD) has potent therapeutic properties, including anti-inflammatory, antioxidant, and neuroprotective properties. In this work, physicochemical characterization was carried out after producing Compritol-based nanoparticles (cSLN or cNLC) loaded with CBD. Then, the determination of the encapsulation efficiency (EE), loading capacity (LC), particle size (Z-Ave), polydispersity index (PDI), and zeta potential were performed. Additionally, the viscoelastic profiles and differential scanning calorimetry (DSC) patterns were recorded. As a result, CBD-loaded SLN showed a mean particle size of 217.2 ± 6.5 nm, PDI of 0.273 ± 0.023, and EE of about 74%, while CBD-loaded NLC showed Z-Ave of 158.3 ± 6.6 nm, PDI of 0.325 ± 0.016, and EE of about 70%. The rheological analysis showed that the loss modulus for both lipid nanoparticle formulations was higher than the storage modulus over the applied frequency range of 10 Hz, demonstrating that they are more elastic than viscous. The crystallinity profiles of both CBD-cSLN (90.41%) and CBD-cNLC (40.18%) were determined. It may justify the obtained encapsulation parameters while corroborating the liquid-like character demonstrated in the rheological analysis. Scanning electron microscopy (SEM) study confirmed the morphology and shape of the developed nanoparticles. The work has proven that the solid nature and morphology of cSLN/cNLC strengthen these particles’ potential to modify the CBD delivery profile for several biomedical applications. MDPI 2023-03-22 /pmc/articles/PMC10058297/ /pubmed/36985847 http://dx.doi.org/10.3390/molecules28062875 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zielińska, Aleksandra
da Ana, Raquel
Fonseca, Joel
Szalata, Milena
Wielgus, Karolina
Fathi, Faezeh
Oliveira, M. Beatriz P. P.
Staszewski, Rafał
Karczewski, Jacek
Souto, Eliana B.
Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles
title Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles
title_full Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles
title_fullStr Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles
title_full_unstemmed Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles
title_short Phytocannabinoids: Chromatographic Screening of Cannabinoids and Loading into Lipid Nanoparticles
title_sort phytocannabinoids: chromatographic screening of cannabinoids and loading into lipid nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058297/
https://www.ncbi.nlm.nih.gov/pubmed/36985847
http://dx.doi.org/10.3390/molecules28062875
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