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Efficacy of Fungi in the Decolorization and Detoxification of Remazol Brilliant Blue Dye in Aquatic Environments

Industrial effluents result in water pollution and affect the biological activity of aquatic and terrestrial life. In this study, efficient fungal strains were isolated from the aquatic environment and identified as Aspergillus fumigatus (SN8c) and Aspergillus terreus (SN40b). The isolates were sele...

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Autores principales: , Nabeela, Khan, Sumera Afzal, Mehmood, Saadat, Shabbir, Sohaib Bin, Ali, Sajid, Alrefaei, Abdulwahed Fahad, Albeshr, Mohammed Fahad, Hamayun, Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058383/
https://www.ncbi.nlm.nih.gov/pubmed/36985276
http://dx.doi.org/10.3390/microorganisms11030703
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author , Nabeela
Khan, Sumera Afzal
Mehmood, Saadat
Shabbir, Sohaib Bin
Ali, Sajid
Alrefaei, Abdulwahed Fahad
Albeshr, Mohammed Fahad
Hamayun, Muhammad
author_facet , Nabeela
Khan, Sumera Afzal
Mehmood, Saadat
Shabbir, Sohaib Bin
Ali, Sajid
Alrefaei, Abdulwahed Fahad
Albeshr, Mohammed Fahad
Hamayun, Muhammad
author_sort , Nabeela
collection PubMed
description Industrial effluents result in water pollution and affect the biological activity of aquatic and terrestrial life. In this study, efficient fungal strains were isolated from the aquatic environment and identified as Aspergillus fumigatus (SN8c) and Aspergillus terreus (SN40b). The isolates were selected based on their potential to efficiently decolorize and detoxify Remazol brilliant blue (RBB) dye, which is extensively used in different industries. Initially, a total of 70 different fungal isolates were screened. Among these, 19 isolates demonstrated dye decolorization capabilities, and SN8c and SN40b revealed the highest decolorization capabilities in liquid medium. The maximum estimated decolorization for SN8c was 91.3% and for SN40b, 84.5% at 40 mg/L of RBB dye in the presence of glucose (1 gm/L), after 5 days of incubation at different levels of pH, temperature, nutrient source, and concentration. RBB dye decolorization using SN8c and SN40b isolates was at a maximum of 99% at pH 3–5, whereas minimum decolorization was recorded as 71.29% and 73.4% SN8c, respectively, at pH 11. The maximum decolorization of the dye was 93% and 90.9% in a defined glucose concentration of 1 gm/L, and a 63.01% decrease was recorded in the decolorization activity at a low level of glucose concentration (0.2 gm/L). Finally, the decolorization and degradation were detected using UV spectrometry and HPLC. Toxicity tests of pure dye and treated dye samples were checked against the seed germination of different plants and the larvae mortality of Artemia salina. This study revealed that indigenous aquatic fungal flora can recover contaminated sites and support aquatic and terrestrial life.
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spelling pubmed-100583832023-03-30 Efficacy of Fungi in the Decolorization and Detoxification of Remazol Brilliant Blue Dye in Aquatic Environments , Nabeela Khan, Sumera Afzal Mehmood, Saadat Shabbir, Sohaib Bin Ali, Sajid Alrefaei, Abdulwahed Fahad Albeshr, Mohammed Fahad Hamayun, Muhammad Microorganisms Article Industrial effluents result in water pollution and affect the biological activity of aquatic and terrestrial life. In this study, efficient fungal strains were isolated from the aquatic environment and identified as Aspergillus fumigatus (SN8c) and Aspergillus terreus (SN40b). The isolates were selected based on their potential to efficiently decolorize and detoxify Remazol brilliant blue (RBB) dye, which is extensively used in different industries. Initially, a total of 70 different fungal isolates were screened. Among these, 19 isolates demonstrated dye decolorization capabilities, and SN8c and SN40b revealed the highest decolorization capabilities in liquid medium. The maximum estimated decolorization for SN8c was 91.3% and for SN40b, 84.5% at 40 mg/L of RBB dye in the presence of glucose (1 gm/L), after 5 days of incubation at different levels of pH, temperature, nutrient source, and concentration. RBB dye decolorization using SN8c and SN40b isolates was at a maximum of 99% at pH 3–5, whereas minimum decolorization was recorded as 71.29% and 73.4% SN8c, respectively, at pH 11. The maximum decolorization of the dye was 93% and 90.9% in a defined glucose concentration of 1 gm/L, and a 63.01% decrease was recorded in the decolorization activity at a low level of glucose concentration (0.2 gm/L). Finally, the decolorization and degradation were detected using UV spectrometry and HPLC. Toxicity tests of pure dye and treated dye samples were checked against the seed germination of different plants and the larvae mortality of Artemia salina. This study revealed that indigenous aquatic fungal flora can recover contaminated sites and support aquatic and terrestrial life. MDPI 2023-03-09 /pmc/articles/PMC10058383/ /pubmed/36985276 http://dx.doi.org/10.3390/microorganisms11030703 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
, Nabeela
Khan, Sumera Afzal
Mehmood, Saadat
Shabbir, Sohaib Bin
Ali, Sajid
Alrefaei, Abdulwahed Fahad
Albeshr, Mohammed Fahad
Hamayun, Muhammad
Efficacy of Fungi in the Decolorization and Detoxification of Remazol Brilliant Blue Dye in Aquatic Environments
title Efficacy of Fungi in the Decolorization and Detoxification of Remazol Brilliant Blue Dye in Aquatic Environments
title_full Efficacy of Fungi in the Decolorization and Detoxification of Remazol Brilliant Blue Dye in Aquatic Environments
title_fullStr Efficacy of Fungi in the Decolorization and Detoxification of Remazol Brilliant Blue Dye in Aquatic Environments
title_full_unstemmed Efficacy of Fungi in the Decolorization and Detoxification of Remazol Brilliant Blue Dye in Aquatic Environments
title_short Efficacy of Fungi in the Decolorization and Detoxification of Remazol Brilliant Blue Dye in Aquatic Environments
title_sort efficacy of fungi in the decolorization and detoxification of remazol brilliant blue dye in aquatic environments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058383/
https://www.ncbi.nlm.nih.gov/pubmed/36985276
http://dx.doi.org/10.3390/microorganisms11030703
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