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Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels

This study aimed to evaluate the effects of flavonoids incorporated into poly(N-vinylcaprolactam) (PNVCL) hydrogel on cell viability and mineralization markers of odontoblast-like cells. MDPC-23 cells were exposed to ampelopsin (AMP), isoquercitrin (ISO), rutin (RUT) and control calcium hydroxide (C...

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Autores principales: Braga, Gabriela Pacheco de Almeida, Caiaffa, Karina Sampaio, Rabelo, Rafaela Laruzo, dos Santos, Vanessa Rodrigues, Souza, Amanda Caselato Andolfatto, Ribeiro, Lucas da Silva, de Camargo, Emerson Rodrigues, Prakki, Anuradha, Duque, Cristiane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058549/
https://www.ncbi.nlm.nih.gov/pubmed/36976063
http://dx.doi.org/10.3390/jfb14030139
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author Braga, Gabriela Pacheco de Almeida
Caiaffa, Karina Sampaio
Rabelo, Rafaela Laruzo
dos Santos, Vanessa Rodrigues
Souza, Amanda Caselato Andolfatto
Ribeiro, Lucas da Silva
de Camargo, Emerson Rodrigues
Prakki, Anuradha
Duque, Cristiane
author_facet Braga, Gabriela Pacheco de Almeida
Caiaffa, Karina Sampaio
Rabelo, Rafaela Laruzo
dos Santos, Vanessa Rodrigues
Souza, Amanda Caselato Andolfatto
Ribeiro, Lucas da Silva
de Camargo, Emerson Rodrigues
Prakki, Anuradha
Duque, Cristiane
author_sort Braga, Gabriela Pacheco de Almeida
collection PubMed
description This study aimed to evaluate the effects of flavonoids incorporated into poly(N-vinylcaprolactam) (PNVCL) hydrogel on cell viability and mineralization markers of odontoblast-like cells. MDPC-23 cells were exposed to ampelopsin (AMP), isoquercitrin (ISO), rutin (RUT) and control calcium hydroxide (CH) for evaluation of cell viability, total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition by colorimetric assays. Based on an initial screening, AMP and CH were loaded into PNVCL hydrogels and had their cytotoxicity and effect on mineralization markers determined. Cell viability was above 70% when MDPC-23 cells were treated with AMP, ISO and RUT. AMP showed the highest ALP activity and mineralized nodule deposition. Extracts of PNVCL+AMP and PNVCL+CH in culture medium (at the dilutions of 1/16 and 1/32) did not affect cell viability and stimulated ALP activity and mineralized nodules’ deposition, which were statistically higher than the control in osteogenic medium. In conclusion, AMP and AMP-loaded PNVCL hydrogels were cytocompatible and able to induce bio-mineralization markers in odontoblast-cells.
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spelling pubmed-100585492023-03-30 Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels Braga, Gabriela Pacheco de Almeida Caiaffa, Karina Sampaio Rabelo, Rafaela Laruzo dos Santos, Vanessa Rodrigues Souza, Amanda Caselato Andolfatto Ribeiro, Lucas da Silva de Camargo, Emerson Rodrigues Prakki, Anuradha Duque, Cristiane J Funct Biomater Article This study aimed to evaluate the effects of flavonoids incorporated into poly(N-vinylcaprolactam) (PNVCL) hydrogel on cell viability and mineralization markers of odontoblast-like cells. MDPC-23 cells were exposed to ampelopsin (AMP), isoquercitrin (ISO), rutin (RUT) and control calcium hydroxide (CH) for evaluation of cell viability, total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition by colorimetric assays. Based on an initial screening, AMP and CH were loaded into PNVCL hydrogels and had their cytotoxicity and effect on mineralization markers determined. Cell viability was above 70% when MDPC-23 cells were treated with AMP, ISO and RUT. AMP showed the highest ALP activity and mineralized nodule deposition. Extracts of PNVCL+AMP and PNVCL+CH in culture medium (at the dilutions of 1/16 and 1/32) did not affect cell viability and stimulated ALP activity and mineralized nodules’ deposition, which were statistically higher than the control in osteogenic medium. In conclusion, AMP and AMP-loaded PNVCL hydrogels were cytocompatible and able to induce bio-mineralization markers in odontoblast-cells. MDPI 2023-03-02 /pmc/articles/PMC10058549/ /pubmed/36976063 http://dx.doi.org/10.3390/jfb14030139 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Braga, Gabriela Pacheco de Almeida
Caiaffa, Karina Sampaio
Rabelo, Rafaela Laruzo
dos Santos, Vanessa Rodrigues
Souza, Amanda Caselato Andolfatto
Ribeiro, Lucas da Silva
de Camargo, Emerson Rodrigues
Prakki, Anuradha
Duque, Cristiane
Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels
title Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels
title_full Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels
title_fullStr Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels
title_full_unstemmed Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels
title_short Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels
title_sort cytotoxicity and biomineralization potential of flavonoids incorporated into pnvcl hydrogels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058549/
https://www.ncbi.nlm.nih.gov/pubmed/36976063
http://dx.doi.org/10.3390/jfb14030139
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