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Metabolic Features of Increased Gut Permeability, Inflammation, and Altered Energy Metabolism Distinguish Agricultural Workers at Risk for Mesoamerican Nephropathy

Mesoamerican nephropathy (MeN) is a form of chronic kidney disease found predominantly in young men in Mesoamerica. Strenuous agricultural labor is a consistent risk factor for MeN, but the pathophysiologic mechanism leading to disease is poorly understood. We compared the urine metabolome among men...

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Autores principales: Raines, Nathan H., Leone, Dominick A., O’Callaghan-Gordo, Cristina, Ramirez-Rubio, Oriana, Amador, Juan José, Lopez Pilarte, Damaris, Delgado, Iris S., Leibler, Jessica H., Embade, Nieves, Gil-Redondo, Rubén, Bruzzone, Chiara, Bizkarguenaga, Maider, Scammell, Madeleine K., Parikh, Samir M., Millet, Oscar, Brooks, Daniel R., Friedman, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058628/
https://www.ncbi.nlm.nih.gov/pubmed/36984765
http://dx.doi.org/10.3390/metabo13030325
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author Raines, Nathan H.
Leone, Dominick A.
O’Callaghan-Gordo, Cristina
Ramirez-Rubio, Oriana
Amador, Juan José
Lopez Pilarte, Damaris
Delgado, Iris S.
Leibler, Jessica H.
Embade, Nieves
Gil-Redondo, Rubén
Bruzzone, Chiara
Bizkarguenaga, Maider
Scammell, Madeleine K.
Parikh, Samir M.
Millet, Oscar
Brooks, Daniel R.
Friedman, David J.
author_facet Raines, Nathan H.
Leone, Dominick A.
O’Callaghan-Gordo, Cristina
Ramirez-Rubio, Oriana
Amador, Juan José
Lopez Pilarte, Damaris
Delgado, Iris S.
Leibler, Jessica H.
Embade, Nieves
Gil-Redondo, Rubén
Bruzzone, Chiara
Bizkarguenaga, Maider
Scammell, Madeleine K.
Parikh, Samir M.
Millet, Oscar
Brooks, Daniel R.
Friedman, David J.
author_sort Raines, Nathan H.
collection PubMed
description Mesoamerican nephropathy (MeN) is a form of chronic kidney disease found predominantly in young men in Mesoamerica. Strenuous agricultural labor is a consistent risk factor for MeN, but the pathophysiologic mechanism leading to disease is poorly understood. We compared the urine metabolome among men in Nicaragua engaged in sugarcane harvest and seed cutting (n = 117), a group at high risk for MeN, against three referents: Nicaraguans working less strenuous jobs at the same sugarcane plantations (n = 78); Nicaraguans performing non-agricultural work (n = 102); and agricultural workers in Spain (n = 78). Using proton nuclear magnetic resonance, we identified 136 metabolites among participants. Our non-hypothesis-based approach identified distinguishing urine metabolic features in the high-risk group, revealing increased levels of hippurate and other gut-derived metabolites and decreased metabolites related to central energy metabolism when compared to referent groups. Our complementary hypothesis-based approach, focused on nicotinamide adenine dinucleotide (NAD+) related metabolites, and revealed a higher kynurenate/tryptophan ratio in the high-risk group (p = 0.001), consistent with a heightened inflammatory state. Workers in high-risk occupations are distinguishable by urinary metabolic features that suggest increased gut permeability, inflammation, and altered energy metabolism. Further study is needed to explore the pathophysiologic implications of these findings.
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spelling pubmed-100586282023-03-30 Metabolic Features of Increased Gut Permeability, Inflammation, and Altered Energy Metabolism Distinguish Agricultural Workers at Risk for Mesoamerican Nephropathy Raines, Nathan H. Leone, Dominick A. O’Callaghan-Gordo, Cristina Ramirez-Rubio, Oriana Amador, Juan José Lopez Pilarte, Damaris Delgado, Iris S. Leibler, Jessica H. Embade, Nieves Gil-Redondo, Rubén Bruzzone, Chiara Bizkarguenaga, Maider Scammell, Madeleine K. Parikh, Samir M. Millet, Oscar Brooks, Daniel R. Friedman, David J. Metabolites Article Mesoamerican nephropathy (MeN) is a form of chronic kidney disease found predominantly in young men in Mesoamerica. Strenuous agricultural labor is a consistent risk factor for MeN, but the pathophysiologic mechanism leading to disease is poorly understood. We compared the urine metabolome among men in Nicaragua engaged in sugarcane harvest and seed cutting (n = 117), a group at high risk for MeN, against three referents: Nicaraguans working less strenuous jobs at the same sugarcane plantations (n = 78); Nicaraguans performing non-agricultural work (n = 102); and agricultural workers in Spain (n = 78). Using proton nuclear magnetic resonance, we identified 136 metabolites among participants. Our non-hypothesis-based approach identified distinguishing urine metabolic features in the high-risk group, revealing increased levels of hippurate and other gut-derived metabolites and decreased metabolites related to central energy metabolism when compared to referent groups. Our complementary hypothesis-based approach, focused on nicotinamide adenine dinucleotide (NAD+) related metabolites, and revealed a higher kynurenate/tryptophan ratio in the high-risk group (p = 0.001), consistent with a heightened inflammatory state. Workers in high-risk occupations are distinguishable by urinary metabolic features that suggest increased gut permeability, inflammation, and altered energy metabolism. Further study is needed to explore the pathophysiologic implications of these findings. MDPI 2023-02-22 /pmc/articles/PMC10058628/ /pubmed/36984765 http://dx.doi.org/10.3390/metabo13030325 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Raines, Nathan H.
Leone, Dominick A.
O’Callaghan-Gordo, Cristina
Ramirez-Rubio, Oriana
Amador, Juan José
Lopez Pilarte, Damaris
Delgado, Iris S.
Leibler, Jessica H.
Embade, Nieves
Gil-Redondo, Rubén
Bruzzone, Chiara
Bizkarguenaga, Maider
Scammell, Madeleine K.
Parikh, Samir M.
Millet, Oscar
Brooks, Daniel R.
Friedman, David J.
Metabolic Features of Increased Gut Permeability, Inflammation, and Altered Energy Metabolism Distinguish Agricultural Workers at Risk for Mesoamerican Nephropathy
title Metabolic Features of Increased Gut Permeability, Inflammation, and Altered Energy Metabolism Distinguish Agricultural Workers at Risk for Mesoamerican Nephropathy
title_full Metabolic Features of Increased Gut Permeability, Inflammation, and Altered Energy Metabolism Distinguish Agricultural Workers at Risk for Mesoamerican Nephropathy
title_fullStr Metabolic Features of Increased Gut Permeability, Inflammation, and Altered Energy Metabolism Distinguish Agricultural Workers at Risk for Mesoamerican Nephropathy
title_full_unstemmed Metabolic Features of Increased Gut Permeability, Inflammation, and Altered Energy Metabolism Distinguish Agricultural Workers at Risk for Mesoamerican Nephropathy
title_short Metabolic Features of Increased Gut Permeability, Inflammation, and Altered Energy Metabolism Distinguish Agricultural Workers at Risk for Mesoamerican Nephropathy
title_sort metabolic features of increased gut permeability, inflammation, and altered energy metabolism distinguish agricultural workers at risk for mesoamerican nephropathy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058628/
https://www.ncbi.nlm.nih.gov/pubmed/36984765
http://dx.doi.org/10.3390/metabo13030325
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