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Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms
The effects of nodulation on N metabolism in soybean plants supplied with various forms of N are not fully understood. Ureides are the principal forms of N transported from nodules, but nitrate and asparagine are the primary N compounds transported from roots supplied with NO(3)(−). In this research...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058755/ https://www.ncbi.nlm.nih.gov/pubmed/36984759 http://dx.doi.org/10.3390/metabo13030319 |
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author | Ohyama, Takuji Isaka, Miyuki Saito, Akihiro Higuchi, Kyoko |
author_facet | Ohyama, Takuji Isaka, Miyuki Saito, Akihiro Higuchi, Kyoko |
author_sort | Ohyama, Takuji |
collection | PubMed |
description | The effects of nodulation on N metabolism in soybean plants supplied with various forms of N are not fully understood. Ureides are the principal forms of N transported from nodules, but nitrate and asparagine are the primary N compounds transported from roots supplied with NO(3)(−). In this research, the effects of 1-day treatments of NO(3)(−), NH(4)(+), urea, or NO(3)(−) + NH(4)(+) on N metabolite concentrations in xylem sap and each organ were compared between nodulated and non-nodulated soybeans. Capillary electrophoresis and colorimetry were used for the analysis. In the xylem sap of the nodulated plants with an N-free solution, ureides were the major N metabolites, followed by asparagine and glutamine. Ureides concentrations were much lower in the xylem sap of the non-nodulated soybeans. In the NO(3)(−) treatment, the concentrations of ureides in the xylem sap of the nodulated plants decreased compared to the control plants. In the NH(4)(+), urea, and NO(3)(−) + NH(4)(+) treatments, the concentrations of asparagine and glutamine increased significantly compared with the control and NO(3)(−) treatments. Similar changes with the N treatments were observed between the nodulated and non-nodulated soybeans, suggesting that nodulation does not have significant effects on the metabolism of absorbed N in roots. |
format | Online Article Text |
id | pubmed-10058755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100587552023-03-30 Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms Ohyama, Takuji Isaka, Miyuki Saito, Akihiro Higuchi, Kyoko Metabolites Article The effects of nodulation on N metabolism in soybean plants supplied with various forms of N are not fully understood. Ureides are the principal forms of N transported from nodules, but nitrate and asparagine are the primary N compounds transported from roots supplied with NO(3)(−). In this research, the effects of 1-day treatments of NO(3)(−), NH(4)(+), urea, or NO(3)(−) + NH(4)(+) on N metabolite concentrations in xylem sap and each organ were compared between nodulated and non-nodulated soybeans. Capillary electrophoresis and colorimetry were used for the analysis. In the xylem sap of the nodulated plants with an N-free solution, ureides were the major N metabolites, followed by asparagine and glutamine. Ureides concentrations were much lower in the xylem sap of the non-nodulated soybeans. In the NO(3)(−) treatment, the concentrations of ureides in the xylem sap of the nodulated plants decreased compared to the control plants. In the NH(4)(+), urea, and NO(3)(−) + NH(4)(+) treatments, the concentrations of asparagine and glutamine increased significantly compared with the control and NO(3)(−) treatments. Similar changes with the N treatments were observed between the nodulated and non-nodulated soybeans, suggesting that nodulation does not have significant effects on the metabolism of absorbed N in roots. MDPI 2023-02-21 /pmc/articles/PMC10058755/ /pubmed/36984759 http://dx.doi.org/10.3390/metabo13030319 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ohyama, Takuji Isaka, Miyuki Saito, Akihiro Higuchi, Kyoko Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms |
title | Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms |
title_full | Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms |
title_fullStr | Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms |
title_full_unstemmed | Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms |
title_short | Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms |
title_sort | effects of nodulation on metabolite concentrations in xylem sap and in the organs of soybean plants supplied with different n forms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058755/ https://www.ncbi.nlm.nih.gov/pubmed/36984759 http://dx.doi.org/10.3390/metabo13030319 |
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