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Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms

The effects of nodulation on N metabolism in soybean plants supplied with various forms of N are not fully understood. Ureides are the principal forms of N transported from nodules, but nitrate and asparagine are the primary N compounds transported from roots supplied with NO(3)(−). In this research...

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Autores principales: Ohyama, Takuji, Isaka, Miyuki, Saito, Akihiro, Higuchi, Kyoko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058755/
https://www.ncbi.nlm.nih.gov/pubmed/36984759
http://dx.doi.org/10.3390/metabo13030319
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author Ohyama, Takuji
Isaka, Miyuki
Saito, Akihiro
Higuchi, Kyoko
author_facet Ohyama, Takuji
Isaka, Miyuki
Saito, Akihiro
Higuchi, Kyoko
author_sort Ohyama, Takuji
collection PubMed
description The effects of nodulation on N metabolism in soybean plants supplied with various forms of N are not fully understood. Ureides are the principal forms of N transported from nodules, but nitrate and asparagine are the primary N compounds transported from roots supplied with NO(3)(−). In this research, the effects of 1-day treatments of NO(3)(−), NH(4)(+), urea, or NO(3)(−) + NH(4)(+) on N metabolite concentrations in xylem sap and each organ were compared between nodulated and non-nodulated soybeans. Capillary electrophoresis and colorimetry were used for the analysis. In the xylem sap of the nodulated plants with an N-free solution, ureides were the major N metabolites, followed by asparagine and glutamine. Ureides concentrations were much lower in the xylem sap of the non-nodulated soybeans. In the NO(3)(−) treatment, the concentrations of ureides in the xylem sap of the nodulated plants decreased compared to the control plants. In the NH(4)(+), urea, and NO(3)(−) + NH(4)(+) treatments, the concentrations of asparagine and glutamine increased significantly compared with the control and NO(3)(−) treatments. Similar changes with the N treatments were observed between the nodulated and non-nodulated soybeans, suggesting that nodulation does not have significant effects on the metabolism of absorbed N in roots.
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spelling pubmed-100587552023-03-30 Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms Ohyama, Takuji Isaka, Miyuki Saito, Akihiro Higuchi, Kyoko Metabolites Article The effects of nodulation on N metabolism in soybean plants supplied with various forms of N are not fully understood. Ureides are the principal forms of N transported from nodules, but nitrate and asparagine are the primary N compounds transported from roots supplied with NO(3)(−). In this research, the effects of 1-day treatments of NO(3)(−), NH(4)(+), urea, or NO(3)(−) + NH(4)(+) on N metabolite concentrations in xylem sap and each organ were compared between nodulated and non-nodulated soybeans. Capillary electrophoresis and colorimetry were used for the analysis. In the xylem sap of the nodulated plants with an N-free solution, ureides were the major N metabolites, followed by asparagine and glutamine. Ureides concentrations were much lower in the xylem sap of the non-nodulated soybeans. In the NO(3)(−) treatment, the concentrations of ureides in the xylem sap of the nodulated plants decreased compared to the control plants. In the NH(4)(+), urea, and NO(3)(−) + NH(4)(+) treatments, the concentrations of asparagine and glutamine increased significantly compared with the control and NO(3)(−) treatments. Similar changes with the N treatments were observed between the nodulated and non-nodulated soybeans, suggesting that nodulation does not have significant effects on the metabolism of absorbed N in roots. MDPI 2023-02-21 /pmc/articles/PMC10058755/ /pubmed/36984759 http://dx.doi.org/10.3390/metabo13030319 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ohyama, Takuji
Isaka, Miyuki
Saito, Akihiro
Higuchi, Kyoko
Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms
title Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms
title_full Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms
title_fullStr Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms
title_full_unstemmed Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms
title_short Effects of Nodulation on Metabolite Concentrations in Xylem Sap and in the Organs of Soybean Plants Supplied with Different N Forms
title_sort effects of nodulation on metabolite concentrations in xylem sap and in the organs of soybean plants supplied with different n forms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058755/
https://www.ncbi.nlm.nih.gov/pubmed/36984759
http://dx.doi.org/10.3390/metabo13030319
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