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Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion?

Prevention of bacterial adhesion is a way to reduce and/or avoid biofilm formation, thus restraining its associated infections. The development of repellent anti-adhesive surfaces, such as superhydrophobic surfaces, can be a strategy to avoid bacterial adhesion. In this study, a polyethylene terepht...

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Autores principales: Caykara, Tugce, Fernandes, Sara, Braga, Adelaide, Rodrigues, Joana, Rodrigues, Ligia R., Silva, Carla Joana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058955/
https://www.ncbi.nlm.nih.gov/pubmed/36986011
http://dx.doi.org/10.3390/nano13061117
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author Caykara, Tugce
Fernandes, Sara
Braga, Adelaide
Rodrigues, Joana
Rodrigues, Ligia R.
Silva, Carla Joana
author_facet Caykara, Tugce
Fernandes, Sara
Braga, Adelaide
Rodrigues, Joana
Rodrigues, Ligia R.
Silva, Carla Joana
author_sort Caykara, Tugce
collection PubMed
description Prevention of bacterial adhesion is a way to reduce and/or avoid biofilm formation, thus restraining its associated infections. The development of repellent anti-adhesive surfaces, such as superhydrophobic surfaces, can be a strategy to avoid bacterial adhesion. In this study, a polyethylene terephthalate (PET) film was modified by in situ growth of silica nanoparticles (NPs) to create a rough surface. The surface was further modified with fluorinated carbon chains to increase its hydrophobicity. The modified PET surfaces presented a pronounced superhydrophobic character, showing a water contact angle of 156° and a roughness of 104 nm (a considerable increase comparing with the 69° and 4.8 nm obtained for the untreated PET). Scanning Electron Microscopy was used to evaluate the modified surfaces morphology, further confirming its successful modification with nanoparticles. Additionally, a bacterial adhesion assay using an Escherichia coli expressing YadA, an adhesive protein from Yersinia so-called Yersinia adhesin A, was used to assess the anti-adhesive potential of the modified PET. Contrarily to what was expected, adhesion of E. coli YadA was found to increase on the modified PET surfaces, exhibiting a clear preference for the crevices. This study highlights the role of material micro topography as an important attribute when considering bacterial adhesion.
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spelling pubmed-100589552023-03-30 Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion? Caykara, Tugce Fernandes, Sara Braga, Adelaide Rodrigues, Joana Rodrigues, Ligia R. Silva, Carla Joana Nanomaterials (Basel) Article Prevention of bacterial adhesion is a way to reduce and/or avoid biofilm formation, thus restraining its associated infections. The development of repellent anti-adhesive surfaces, such as superhydrophobic surfaces, can be a strategy to avoid bacterial adhesion. In this study, a polyethylene terephthalate (PET) film was modified by in situ growth of silica nanoparticles (NPs) to create a rough surface. The surface was further modified with fluorinated carbon chains to increase its hydrophobicity. The modified PET surfaces presented a pronounced superhydrophobic character, showing a water contact angle of 156° and a roughness of 104 nm (a considerable increase comparing with the 69° and 4.8 nm obtained for the untreated PET). Scanning Electron Microscopy was used to evaluate the modified surfaces morphology, further confirming its successful modification with nanoparticles. Additionally, a bacterial adhesion assay using an Escherichia coli expressing YadA, an adhesive protein from Yersinia so-called Yersinia adhesin A, was used to assess the anti-adhesive potential of the modified PET. Contrarily to what was expected, adhesion of E. coli YadA was found to increase on the modified PET surfaces, exhibiting a clear preference for the crevices. This study highlights the role of material micro topography as an important attribute when considering bacterial adhesion. MDPI 2023-03-21 /pmc/articles/PMC10058955/ /pubmed/36986011 http://dx.doi.org/10.3390/nano13061117 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Caykara, Tugce
Fernandes, Sara
Braga, Adelaide
Rodrigues, Joana
Rodrigues, Ligia R.
Silva, Carla Joana
Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion?
title Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion?
title_full Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion?
title_fullStr Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion?
title_full_unstemmed Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion?
title_short Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion?
title_sort can superhydrophobic pet surfaces prevent bacterial adhesion?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058955/
https://www.ncbi.nlm.nih.gov/pubmed/36986011
http://dx.doi.org/10.3390/nano13061117
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