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Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion?
Prevention of bacterial adhesion is a way to reduce and/or avoid biofilm formation, thus restraining its associated infections. The development of repellent anti-adhesive surfaces, such as superhydrophobic surfaces, can be a strategy to avoid bacterial adhesion. In this study, a polyethylene terepht...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058955/ https://www.ncbi.nlm.nih.gov/pubmed/36986011 http://dx.doi.org/10.3390/nano13061117 |
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author | Caykara, Tugce Fernandes, Sara Braga, Adelaide Rodrigues, Joana Rodrigues, Ligia R. Silva, Carla Joana |
author_facet | Caykara, Tugce Fernandes, Sara Braga, Adelaide Rodrigues, Joana Rodrigues, Ligia R. Silva, Carla Joana |
author_sort | Caykara, Tugce |
collection | PubMed |
description | Prevention of bacterial adhesion is a way to reduce and/or avoid biofilm formation, thus restraining its associated infections. The development of repellent anti-adhesive surfaces, such as superhydrophobic surfaces, can be a strategy to avoid bacterial adhesion. In this study, a polyethylene terephthalate (PET) film was modified by in situ growth of silica nanoparticles (NPs) to create a rough surface. The surface was further modified with fluorinated carbon chains to increase its hydrophobicity. The modified PET surfaces presented a pronounced superhydrophobic character, showing a water contact angle of 156° and a roughness of 104 nm (a considerable increase comparing with the 69° and 4.8 nm obtained for the untreated PET). Scanning Electron Microscopy was used to evaluate the modified surfaces morphology, further confirming its successful modification with nanoparticles. Additionally, a bacterial adhesion assay using an Escherichia coli expressing YadA, an adhesive protein from Yersinia so-called Yersinia adhesin A, was used to assess the anti-adhesive potential of the modified PET. Contrarily to what was expected, adhesion of E. coli YadA was found to increase on the modified PET surfaces, exhibiting a clear preference for the crevices. This study highlights the role of material micro topography as an important attribute when considering bacterial adhesion. |
format | Online Article Text |
id | pubmed-10058955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100589552023-03-30 Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion? Caykara, Tugce Fernandes, Sara Braga, Adelaide Rodrigues, Joana Rodrigues, Ligia R. Silva, Carla Joana Nanomaterials (Basel) Article Prevention of bacterial adhesion is a way to reduce and/or avoid biofilm formation, thus restraining its associated infections. The development of repellent anti-adhesive surfaces, such as superhydrophobic surfaces, can be a strategy to avoid bacterial adhesion. In this study, a polyethylene terephthalate (PET) film was modified by in situ growth of silica nanoparticles (NPs) to create a rough surface. The surface was further modified with fluorinated carbon chains to increase its hydrophobicity. The modified PET surfaces presented a pronounced superhydrophobic character, showing a water contact angle of 156° and a roughness of 104 nm (a considerable increase comparing with the 69° and 4.8 nm obtained for the untreated PET). Scanning Electron Microscopy was used to evaluate the modified surfaces morphology, further confirming its successful modification with nanoparticles. Additionally, a bacterial adhesion assay using an Escherichia coli expressing YadA, an adhesive protein from Yersinia so-called Yersinia adhesin A, was used to assess the anti-adhesive potential of the modified PET. Contrarily to what was expected, adhesion of E. coli YadA was found to increase on the modified PET surfaces, exhibiting a clear preference for the crevices. This study highlights the role of material micro topography as an important attribute when considering bacterial adhesion. MDPI 2023-03-21 /pmc/articles/PMC10058955/ /pubmed/36986011 http://dx.doi.org/10.3390/nano13061117 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Caykara, Tugce Fernandes, Sara Braga, Adelaide Rodrigues, Joana Rodrigues, Ligia R. Silva, Carla Joana Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion? |
title | Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion? |
title_full | Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion? |
title_fullStr | Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion? |
title_full_unstemmed | Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion? |
title_short | Can Superhydrophobic PET Surfaces Prevent Bacterial Adhesion? |
title_sort | can superhydrophobic pet surfaces prevent bacterial adhesion? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10058955/ https://www.ncbi.nlm.nih.gov/pubmed/36986011 http://dx.doi.org/10.3390/nano13061117 |
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