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Data Independent Acquisition Reveals In-Depth Serum Proteome Changes in Canine Leishmaniosis
Comprehensive profiling of serum proteome provides valuable clues of health status and pathophysiological processes, making it the main strategy in biomarker discovery. However, the high dynamic range significantly decreases the number of detectable proteins, obstructing the insights into the underl...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10059658/ https://www.ncbi.nlm.nih.gov/pubmed/36984805 http://dx.doi.org/10.3390/metabo13030365 |
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author | Martinković, Franjo Popović, Marin Smolec, Ozren Mrljak, Vladimir Eckersall, Peter David Horvatić, Anita |
author_facet | Martinković, Franjo Popović, Marin Smolec, Ozren Mrljak, Vladimir Eckersall, Peter David Horvatić, Anita |
author_sort | Martinković, Franjo |
collection | PubMed |
description | Comprehensive profiling of serum proteome provides valuable clues of health status and pathophysiological processes, making it the main strategy in biomarker discovery. However, the high dynamic range significantly decreases the number of detectable proteins, obstructing the insights into the underlying biological processes. To circumvent various serum enrichment methods, obtain high-quality proteome wide information using the next-generation proteomic, and study host response in canine leishmaniosis, we applied data-independent acquisition mass spectrometry (DIA-MS) for deep proteomic profiling of clinical samples. The non-depleted serum samples of healthy and naturally Leishmania-infected dogs were analyzed using the label-free 60-min gradient sequential window acquisition of all theoretical mass spectra (SWATH-MS) method. As a result, we identified 554 proteins, 140 of which differed significantly in abundance. Those were included in lipid metabolism, hematological abnormalities, immune response, and oxidative stress, providing valuable information about the complex molecular basis of the clinical and pathological landscape in canine leishmaniosis. Our results show that DIA-MS is a method of choice for understanding complex pathophysiological processes in serum and serum biomarker development. |
format | Online Article Text |
id | pubmed-10059658 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100596582023-03-30 Data Independent Acquisition Reveals In-Depth Serum Proteome Changes in Canine Leishmaniosis Martinković, Franjo Popović, Marin Smolec, Ozren Mrljak, Vladimir Eckersall, Peter David Horvatić, Anita Metabolites Article Comprehensive profiling of serum proteome provides valuable clues of health status and pathophysiological processes, making it the main strategy in biomarker discovery. However, the high dynamic range significantly decreases the number of detectable proteins, obstructing the insights into the underlying biological processes. To circumvent various serum enrichment methods, obtain high-quality proteome wide information using the next-generation proteomic, and study host response in canine leishmaniosis, we applied data-independent acquisition mass spectrometry (DIA-MS) for deep proteomic profiling of clinical samples. The non-depleted serum samples of healthy and naturally Leishmania-infected dogs were analyzed using the label-free 60-min gradient sequential window acquisition of all theoretical mass spectra (SWATH-MS) method. As a result, we identified 554 proteins, 140 of which differed significantly in abundance. Those were included in lipid metabolism, hematological abnormalities, immune response, and oxidative stress, providing valuable information about the complex molecular basis of the clinical and pathological landscape in canine leishmaniosis. Our results show that DIA-MS is a method of choice for understanding complex pathophysiological processes in serum and serum biomarker development. MDPI 2023-02-28 /pmc/articles/PMC10059658/ /pubmed/36984805 http://dx.doi.org/10.3390/metabo13030365 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Martinković, Franjo Popović, Marin Smolec, Ozren Mrljak, Vladimir Eckersall, Peter David Horvatić, Anita Data Independent Acquisition Reveals In-Depth Serum Proteome Changes in Canine Leishmaniosis |
title | Data Independent Acquisition Reveals In-Depth Serum Proteome Changes in Canine Leishmaniosis |
title_full | Data Independent Acquisition Reveals In-Depth Serum Proteome Changes in Canine Leishmaniosis |
title_fullStr | Data Independent Acquisition Reveals In-Depth Serum Proteome Changes in Canine Leishmaniosis |
title_full_unstemmed | Data Independent Acquisition Reveals In-Depth Serum Proteome Changes in Canine Leishmaniosis |
title_short | Data Independent Acquisition Reveals In-Depth Serum Proteome Changes in Canine Leishmaniosis |
title_sort | data independent acquisition reveals in-depth serum proteome changes in canine leishmaniosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10059658/ https://www.ncbi.nlm.nih.gov/pubmed/36984805 http://dx.doi.org/10.3390/metabo13030365 |
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