Cargando…

Characterization and Comparative Genomic Analysis of Three Virulent E. coli Bacteriophages with the Potential to Reduce Antibiotic-Resistant Bacteria in the Environment

The emerging global crisis of antibiotic resistance demands new alternative antibacterial solutions. Although bacteriophages have been used to combat bacterial infections for over a century, a dramatic boost in phage studies has recently been observed. In the development of modern phage applications...

Descripción completa

Detalles Bibliográficos
Autores principales: Śliwka, Paulina, Weber-Dąbrowska, Beata, Żaczek, Maciej, Kuźmińska-Bajor, Marta, Dusza, Izabela, Skaradzińska, Aneta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10059673/
https://www.ncbi.nlm.nih.gov/pubmed/36982770
http://dx.doi.org/10.3390/ijms24065696
_version_ 1785016930088779776
author Śliwka, Paulina
Weber-Dąbrowska, Beata
Żaczek, Maciej
Kuźmińska-Bajor, Marta
Dusza, Izabela
Skaradzińska, Aneta
author_facet Śliwka, Paulina
Weber-Dąbrowska, Beata
Żaczek, Maciej
Kuźmińska-Bajor, Marta
Dusza, Izabela
Skaradzińska, Aneta
author_sort Śliwka, Paulina
collection PubMed
description The emerging global crisis of antibiotic resistance demands new alternative antibacterial solutions. Although bacteriophages have been used to combat bacterial infections for over a century, a dramatic boost in phage studies has recently been observed. In the development of modern phage applications, a scientific rationale is strongly required and newly isolated phages need to be examined in detail. In this study, we present the full characterization of bacteriophages BF9, BF15, and BF17, with lytic activity against extended-spectrum β-lactamases (ESBLs)- and AmpC β-lactamases (AmpC)-producing Escherichia coli, the prevalence of which has increased significantly in livestock in recent decades, representing a great hazard to food safety and a public health risk. Comparative genomic and phylogenetic analysis indicated that BF9, BF15, and BF17 represent the genera Dhillonvirus, Tequatrovirus, and Asteriusvirus, respectively. All three phages significantly reduced in vitro growth of their bacterial host and retained the ability to lyse bacteria after preincubation at wide ranges of temperature (−20–40 °C) and pH (5–9). The results described herein indicate the lytic nature of BF9, BF15, and BF17, which, along with the absence of genes encoding toxins and bacterial virulence factors, represents an undoubted asset in terms of future phage application.
format Online
Article
Text
id pubmed-10059673
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-100596732023-03-30 Characterization and Comparative Genomic Analysis of Three Virulent E. coli Bacteriophages with the Potential to Reduce Antibiotic-Resistant Bacteria in the Environment Śliwka, Paulina Weber-Dąbrowska, Beata Żaczek, Maciej Kuźmińska-Bajor, Marta Dusza, Izabela Skaradzińska, Aneta Int J Mol Sci Article The emerging global crisis of antibiotic resistance demands new alternative antibacterial solutions. Although bacteriophages have been used to combat bacterial infections for over a century, a dramatic boost in phage studies has recently been observed. In the development of modern phage applications, a scientific rationale is strongly required and newly isolated phages need to be examined in detail. In this study, we present the full characterization of bacteriophages BF9, BF15, and BF17, with lytic activity against extended-spectrum β-lactamases (ESBLs)- and AmpC β-lactamases (AmpC)-producing Escherichia coli, the prevalence of which has increased significantly in livestock in recent decades, representing a great hazard to food safety and a public health risk. Comparative genomic and phylogenetic analysis indicated that BF9, BF15, and BF17 represent the genera Dhillonvirus, Tequatrovirus, and Asteriusvirus, respectively. All three phages significantly reduced in vitro growth of their bacterial host and retained the ability to lyse bacteria after preincubation at wide ranges of temperature (−20–40 °C) and pH (5–9). The results described herein indicate the lytic nature of BF9, BF15, and BF17, which, along with the absence of genes encoding toxins and bacterial virulence factors, represents an undoubted asset in terms of future phage application. MDPI 2023-03-16 /pmc/articles/PMC10059673/ /pubmed/36982770 http://dx.doi.org/10.3390/ijms24065696 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Śliwka, Paulina
Weber-Dąbrowska, Beata
Żaczek, Maciej
Kuźmińska-Bajor, Marta
Dusza, Izabela
Skaradzińska, Aneta
Characterization and Comparative Genomic Analysis of Three Virulent E. coli Bacteriophages with the Potential to Reduce Antibiotic-Resistant Bacteria in the Environment
title Characterization and Comparative Genomic Analysis of Three Virulent E. coli Bacteriophages with the Potential to Reduce Antibiotic-Resistant Bacteria in the Environment
title_full Characterization and Comparative Genomic Analysis of Three Virulent E. coli Bacteriophages with the Potential to Reduce Antibiotic-Resistant Bacteria in the Environment
title_fullStr Characterization and Comparative Genomic Analysis of Three Virulent E. coli Bacteriophages with the Potential to Reduce Antibiotic-Resistant Bacteria in the Environment
title_full_unstemmed Characterization and Comparative Genomic Analysis of Three Virulent E. coli Bacteriophages with the Potential to Reduce Antibiotic-Resistant Bacteria in the Environment
title_short Characterization and Comparative Genomic Analysis of Three Virulent E. coli Bacteriophages with the Potential to Reduce Antibiotic-Resistant Bacteria in the Environment
title_sort characterization and comparative genomic analysis of three virulent e. coli bacteriophages with the potential to reduce antibiotic-resistant bacteria in the environment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10059673/
https://www.ncbi.nlm.nih.gov/pubmed/36982770
http://dx.doi.org/10.3390/ijms24065696
work_keys_str_mv AT sliwkapaulina characterizationandcomparativegenomicanalysisofthreevirulentecolibacteriophageswiththepotentialtoreduceantibioticresistantbacteriaintheenvironment
AT weberdabrowskabeata characterizationandcomparativegenomicanalysisofthreevirulentecolibacteriophageswiththepotentialtoreduceantibioticresistantbacteriaintheenvironment
AT zaczekmaciej characterizationandcomparativegenomicanalysisofthreevirulentecolibacteriophageswiththepotentialtoreduceantibioticresistantbacteriaintheenvironment
AT kuzminskabajormarta characterizationandcomparativegenomicanalysisofthreevirulentecolibacteriophageswiththepotentialtoreduceantibioticresistantbacteriaintheenvironment
AT duszaizabela characterizationandcomparativegenomicanalysisofthreevirulentecolibacteriophageswiththepotentialtoreduceantibioticresistantbacteriaintheenvironment
AT skaradzinskaaneta characterizationandcomparativegenomicanalysisofthreevirulentecolibacteriophageswiththepotentialtoreduceantibioticresistantbacteriaintheenvironment