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Acuminosylation of Tyrosol by a Commercial Diglycosidase

A commercial glycosidase mixture obtained from Penicillium multicolor (Aromase H2) was found to comprise a specific diglycosidase activity, β-acuminosidase, alongside undetectable levels of β-apiosidase. The enzyme was tested in the transglycosylation of tyrosol using 4-nitrophenyl β-acuminoside as...

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Autores principales: Haluz, Peter, Kis, Peter, Cvečko, Matej, Mastihubová, Mária, Mastihuba, Vladimír
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10059904/
https://www.ncbi.nlm.nih.gov/pubmed/36983015
http://dx.doi.org/10.3390/ijms24065943
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author Haluz, Peter
Kis, Peter
Cvečko, Matej
Mastihubová, Mária
Mastihuba, Vladimír
author_facet Haluz, Peter
Kis, Peter
Cvečko, Matej
Mastihubová, Mária
Mastihuba, Vladimír
author_sort Haluz, Peter
collection PubMed
description A commercial glycosidase mixture obtained from Penicillium multicolor (Aromase H2) was found to comprise a specific diglycosidase activity, β-acuminosidase, alongside undetectable levels of β-apiosidase. The enzyme was tested in the transglycosylation of tyrosol using 4-nitrophenyl β-acuminoside as the diglycosyl donor. The reaction was not chemoselective, providing a mixture of Osmanthuside H and its counterpart regioisomer 4-(2-hydroxyethyl)phenyl β-acuminoside in 58% yield. Aromase H2 is therefore the first commercial β-acuminosidase which is also able to glycosylate phenolic acceptors.
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spelling pubmed-100599042023-03-30 Acuminosylation of Tyrosol by a Commercial Diglycosidase Haluz, Peter Kis, Peter Cvečko, Matej Mastihubová, Mária Mastihuba, Vladimír Int J Mol Sci Communication A commercial glycosidase mixture obtained from Penicillium multicolor (Aromase H2) was found to comprise a specific diglycosidase activity, β-acuminosidase, alongside undetectable levels of β-apiosidase. The enzyme was tested in the transglycosylation of tyrosol using 4-nitrophenyl β-acuminoside as the diglycosyl donor. The reaction was not chemoselective, providing a mixture of Osmanthuside H and its counterpart regioisomer 4-(2-hydroxyethyl)phenyl β-acuminoside in 58% yield. Aromase H2 is therefore the first commercial β-acuminosidase which is also able to glycosylate phenolic acceptors. MDPI 2023-03-21 /pmc/articles/PMC10059904/ /pubmed/36983015 http://dx.doi.org/10.3390/ijms24065943 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Haluz, Peter
Kis, Peter
Cvečko, Matej
Mastihubová, Mária
Mastihuba, Vladimír
Acuminosylation of Tyrosol by a Commercial Diglycosidase
title Acuminosylation of Tyrosol by a Commercial Diglycosidase
title_full Acuminosylation of Tyrosol by a Commercial Diglycosidase
title_fullStr Acuminosylation of Tyrosol by a Commercial Diglycosidase
title_full_unstemmed Acuminosylation of Tyrosol by a Commercial Diglycosidase
title_short Acuminosylation of Tyrosol by a Commercial Diglycosidase
title_sort acuminosylation of tyrosol by a commercial diglycosidase
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10059904/
https://www.ncbi.nlm.nih.gov/pubmed/36983015
http://dx.doi.org/10.3390/ijms24065943
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