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Acuminosylation of Tyrosol by a Commercial Diglycosidase
A commercial glycosidase mixture obtained from Penicillium multicolor (Aromase H2) was found to comprise a specific diglycosidase activity, β-acuminosidase, alongside undetectable levels of β-apiosidase. The enzyme was tested in the transglycosylation of tyrosol using 4-nitrophenyl β-acuminoside as...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10059904/ https://www.ncbi.nlm.nih.gov/pubmed/36983015 http://dx.doi.org/10.3390/ijms24065943 |
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author | Haluz, Peter Kis, Peter Cvečko, Matej Mastihubová, Mária Mastihuba, Vladimír |
author_facet | Haluz, Peter Kis, Peter Cvečko, Matej Mastihubová, Mária Mastihuba, Vladimír |
author_sort | Haluz, Peter |
collection | PubMed |
description | A commercial glycosidase mixture obtained from Penicillium multicolor (Aromase H2) was found to comprise a specific diglycosidase activity, β-acuminosidase, alongside undetectable levels of β-apiosidase. The enzyme was tested in the transglycosylation of tyrosol using 4-nitrophenyl β-acuminoside as the diglycosyl donor. The reaction was not chemoselective, providing a mixture of Osmanthuside H and its counterpart regioisomer 4-(2-hydroxyethyl)phenyl β-acuminoside in 58% yield. Aromase H2 is therefore the first commercial β-acuminosidase which is also able to glycosylate phenolic acceptors. |
format | Online Article Text |
id | pubmed-10059904 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-100599042023-03-30 Acuminosylation of Tyrosol by a Commercial Diglycosidase Haluz, Peter Kis, Peter Cvečko, Matej Mastihubová, Mária Mastihuba, Vladimír Int J Mol Sci Communication A commercial glycosidase mixture obtained from Penicillium multicolor (Aromase H2) was found to comprise a specific diglycosidase activity, β-acuminosidase, alongside undetectable levels of β-apiosidase. The enzyme was tested in the transglycosylation of tyrosol using 4-nitrophenyl β-acuminoside as the diglycosyl donor. The reaction was not chemoselective, providing a mixture of Osmanthuside H and its counterpart regioisomer 4-(2-hydroxyethyl)phenyl β-acuminoside in 58% yield. Aromase H2 is therefore the first commercial β-acuminosidase which is also able to glycosylate phenolic acceptors. MDPI 2023-03-21 /pmc/articles/PMC10059904/ /pubmed/36983015 http://dx.doi.org/10.3390/ijms24065943 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Haluz, Peter Kis, Peter Cvečko, Matej Mastihubová, Mária Mastihuba, Vladimír Acuminosylation of Tyrosol by a Commercial Diglycosidase |
title | Acuminosylation of Tyrosol by a Commercial Diglycosidase |
title_full | Acuminosylation of Tyrosol by a Commercial Diglycosidase |
title_fullStr | Acuminosylation of Tyrosol by a Commercial Diglycosidase |
title_full_unstemmed | Acuminosylation of Tyrosol by a Commercial Diglycosidase |
title_short | Acuminosylation of Tyrosol by a Commercial Diglycosidase |
title_sort | acuminosylation of tyrosol by a commercial diglycosidase |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10059904/ https://www.ncbi.nlm.nih.gov/pubmed/36983015 http://dx.doi.org/10.3390/ijms24065943 |
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