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Targeted Desorption Electrospray Ionization Mass Spectrometry Imaging for Drug Distribution, Toxicity, and Tissue Classification Studies

With increased use of mass spectrometry imaging (MSI) in support of pharmaceutical research and development, there are opportunities to develop analytical pipelines that incorporate exploratory high-performance analysis with higher capacity and faster targeted MSI. Therefore, to enable faster MSI da...

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Autores principales: Dannhorn, Andreas, Doria, Maria Luisa, McKenzie, James, Inglese, Paolo, Swales, John G., Hamm, Gregory, Strittmatter, Nicole, Maglennon, Gareth, Ghaem-Maghami, Sadaf, Goodwin, Richard J. A., Takats, Zoltan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060000/
https://www.ncbi.nlm.nih.gov/pubmed/36984817
http://dx.doi.org/10.3390/metabo13030377
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author Dannhorn, Andreas
Doria, Maria Luisa
McKenzie, James
Inglese, Paolo
Swales, John G.
Hamm, Gregory
Strittmatter, Nicole
Maglennon, Gareth
Ghaem-Maghami, Sadaf
Goodwin, Richard J. A.
Takats, Zoltan
author_facet Dannhorn, Andreas
Doria, Maria Luisa
McKenzie, James
Inglese, Paolo
Swales, John G.
Hamm, Gregory
Strittmatter, Nicole
Maglennon, Gareth
Ghaem-Maghami, Sadaf
Goodwin, Richard J. A.
Takats, Zoltan
author_sort Dannhorn, Andreas
collection PubMed
description With increased use of mass spectrometry imaging (MSI) in support of pharmaceutical research and development, there are opportunities to develop analytical pipelines that incorporate exploratory high-performance analysis with higher capacity and faster targeted MSI. Therefore, to enable faster MSI data acquisition we present analyte-targeted desorption electrospray ionization–mass spectrometry imaging (DESI-MSI) utilizing a triple-quadrupole (TQ) mass analyzer. The evaluated platform configuration provided superior sensitivity compared to a conventional time-of-flight (TOF) mass analyzer and thus holds the potential to generate data applicable to pharmaceutical research and development. The platform was successfully operated with sampling rates up to 10 scans/s, comparing positively to the 1 scan/s commonly used on comparable DESI-TOF setups. The higher scan rate enabled investigation of the desorption/ionization processes of endogenous lipid species such as phosphatidylcholines and a co-administered cassette of four orally dosed drugs—erlotininb, moxifloxacin, olanzapine, and terfenadine. This was used to enable understanding of the impact of the desorption/ionization processes in order to optimize the operational parameters, resulting in improved compound coverage for olanzapine and the main olanzapine metabolite, hydroxy-olanzapine, in brain tissue sections compared to DESI-TOF analysis or matrix-assisted laser desorption/ionization (MALDI) platforms. The approach allowed reducing the amount of recorded information, thus reducing the size of datasets from up to 150 GB per experiment down to several hundred MB. The improved performance was demonstrated in case studies investigating the suitability of this approach for mapping drug distribution, spatially resolved profiling of drug-induced nephrotoxicity, and molecular–histological tissue classification of ovarian tumors specimens.
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spelling pubmed-100600002023-03-30 Targeted Desorption Electrospray Ionization Mass Spectrometry Imaging for Drug Distribution, Toxicity, and Tissue Classification Studies Dannhorn, Andreas Doria, Maria Luisa McKenzie, James Inglese, Paolo Swales, John G. Hamm, Gregory Strittmatter, Nicole Maglennon, Gareth Ghaem-Maghami, Sadaf Goodwin, Richard J. A. Takats, Zoltan Metabolites Article With increased use of mass spectrometry imaging (MSI) in support of pharmaceutical research and development, there are opportunities to develop analytical pipelines that incorporate exploratory high-performance analysis with higher capacity and faster targeted MSI. Therefore, to enable faster MSI data acquisition we present analyte-targeted desorption electrospray ionization–mass spectrometry imaging (DESI-MSI) utilizing a triple-quadrupole (TQ) mass analyzer. The evaluated platform configuration provided superior sensitivity compared to a conventional time-of-flight (TOF) mass analyzer and thus holds the potential to generate data applicable to pharmaceutical research and development. The platform was successfully operated with sampling rates up to 10 scans/s, comparing positively to the 1 scan/s commonly used on comparable DESI-TOF setups. The higher scan rate enabled investigation of the desorption/ionization processes of endogenous lipid species such as phosphatidylcholines and a co-administered cassette of four orally dosed drugs—erlotininb, moxifloxacin, olanzapine, and terfenadine. This was used to enable understanding of the impact of the desorption/ionization processes in order to optimize the operational parameters, resulting in improved compound coverage for olanzapine and the main olanzapine metabolite, hydroxy-olanzapine, in brain tissue sections compared to DESI-TOF analysis or matrix-assisted laser desorption/ionization (MALDI) platforms. The approach allowed reducing the amount of recorded information, thus reducing the size of datasets from up to 150 GB per experiment down to several hundred MB. The improved performance was demonstrated in case studies investigating the suitability of this approach for mapping drug distribution, spatially resolved profiling of drug-induced nephrotoxicity, and molecular–histological tissue classification of ovarian tumors specimens. MDPI 2023-03-03 /pmc/articles/PMC10060000/ /pubmed/36984817 http://dx.doi.org/10.3390/metabo13030377 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dannhorn, Andreas
Doria, Maria Luisa
McKenzie, James
Inglese, Paolo
Swales, John G.
Hamm, Gregory
Strittmatter, Nicole
Maglennon, Gareth
Ghaem-Maghami, Sadaf
Goodwin, Richard J. A.
Takats, Zoltan
Targeted Desorption Electrospray Ionization Mass Spectrometry Imaging for Drug Distribution, Toxicity, and Tissue Classification Studies
title Targeted Desorption Electrospray Ionization Mass Spectrometry Imaging for Drug Distribution, Toxicity, and Tissue Classification Studies
title_full Targeted Desorption Electrospray Ionization Mass Spectrometry Imaging for Drug Distribution, Toxicity, and Tissue Classification Studies
title_fullStr Targeted Desorption Electrospray Ionization Mass Spectrometry Imaging for Drug Distribution, Toxicity, and Tissue Classification Studies
title_full_unstemmed Targeted Desorption Electrospray Ionization Mass Spectrometry Imaging for Drug Distribution, Toxicity, and Tissue Classification Studies
title_short Targeted Desorption Electrospray Ionization Mass Spectrometry Imaging for Drug Distribution, Toxicity, and Tissue Classification Studies
title_sort targeted desorption electrospray ionization mass spectrometry imaging for drug distribution, toxicity, and tissue classification studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060000/
https://www.ncbi.nlm.nih.gov/pubmed/36984817
http://dx.doi.org/10.3390/metabo13030377
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