Cargando…
A 3D culture system for evaluating the combined effects of cisplatin and anti-fibrotic drugs on the growth and invasion of lung cancer cells co-cultured with fibroblasts
Fibrosis and fibroblast activation usually occur in the tissues surrounding a malignant tumor; therefore, anti-fibrotic drugs are used in addition to chemotherapy. A reliable technique for evaluating the combined effects of anti-fibrotic drugs and anticancer drugs would be beneficial for the develop...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060027/ https://www.ncbi.nlm.nih.gov/pubmed/37006781 http://dx.doi.org/10.1063/5.0115464 |
_version_ | 1785017018213203968 |
---|---|
author | Pan, Huei-Jyuan Lee, Chia-Wei Wu, Li-Yu Hsu, Heng-Hua Tung, Yi-Chung Liao, Wei-Yu Lee, Chau-Hwang |
author_facet | Pan, Huei-Jyuan Lee, Chia-Wei Wu, Li-Yu Hsu, Heng-Hua Tung, Yi-Chung Liao, Wei-Yu Lee, Chau-Hwang |
author_sort | Pan, Huei-Jyuan |
collection | PubMed |
description | Fibrosis and fibroblast activation usually occur in the tissues surrounding a malignant tumor; therefore, anti-fibrotic drugs are used in addition to chemotherapy. A reliable technique for evaluating the combined effects of anti-fibrotic drugs and anticancer drugs would be beneficial for the development of an appropriate treatment strategy. In this study, we manufactured a three-dimensional (3D) co-culture system of fibroblasts and lung cancer cell spheroids in Matrigel supplemented with fibrin (fibrin/Matrigel) that simulated the tissue microenvironment around a solid tumor. We compared the efficacy of an anticancer drug (cisplatin) with or without pretreatments of two anti-fibrotic drugs, nintedanib and pirfenidone, on the growth and invasion of cancer cells co-cultured with fibroblasts. The results showed that the addition of nintedanib improved cisplatin's effects on suppressing the growth of cancer cell spheroids and the invasion of cancer cells. In contrast, pirfenidone did not enhance the anticancer activity of cisplatin. Nintedanib also showed higher efficacy than pirfenidone in reducing the expression of four genes in fibroblasts associated with cell adhesion, invasion, and extracellular matrix degradation. This study demonstrated that the 3D co-cultures in fibrin/Matrigel would be useful for assessing the effects of drug combinations on tumor growth and invasion. |
format | Online Article Text |
id | pubmed-10060027 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-100600272023-03-30 A 3D culture system for evaluating the combined effects of cisplatin and anti-fibrotic drugs on the growth and invasion of lung cancer cells co-cultured with fibroblasts Pan, Huei-Jyuan Lee, Chia-Wei Wu, Li-Yu Hsu, Heng-Hua Tung, Yi-Chung Liao, Wei-Yu Lee, Chau-Hwang APL Bioeng Articles Fibrosis and fibroblast activation usually occur in the tissues surrounding a malignant tumor; therefore, anti-fibrotic drugs are used in addition to chemotherapy. A reliable technique for evaluating the combined effects of anti-fibrotic drugs and anticancer drugs would be beneficial for the development of an appropriate treatment strategy. In this study, we manufactured a three-dimensional (3D) co-culture system of fibroblasts and lung cancer cell spheroids in Matrigel supplemented with fibrin (fibrin/Matrigel) that simulated the tissue microenvironment around a solid tumor. We compared the efficacy of an anticancer drug (cisplatin) with or without pretreatments of two anti-fibrotic drugs, nintedanib and pirfenidone, on the growth and invasion of cancer cells co-cultured with fibroblasts. The results showed that the addition of nintedanib improved cisplatin's effects on suppressing the growth of cancer cell spheroids and the invasion of cancer cells. In contrast, pirfenidone did not enhance the anticancer activity of cisplatin. Nintedanib also showed higher efficacy than pirfenidone in reducing the expression of four genes in fibroblasts associated with cell adhesion, invasion, and extracellular matrix degradation. This study demonstrated that the 3D co-cultures in fibrin/Matrigel would be useful for assessing the effects of drug combinations on tumor growth and invasion. AIP Publishing LLC 2023-03-28 /pmc/articles/PMC10060027/ /pubmed/37006781 http://dx.doi.org/10.1063/5.0115464 Text en © 2023 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Articles Pan, Huei-Jyuan Lee, Chia-Wei Wu, Li-Yu Hsu, Heng-Hua Tung, Yi-Chung Liao, Wei-Yu Lee, Chau-Hwang A 3D culture system for evaluating the combined effects of cisplatin and anti-fibrotic drugs on the growth and invasion of lung cancer cells co-cultured with fibroblasts |
title | A 3D culture system for evaluating the combined effects of cisplatin and anti-fibrotic drugs on the growth and invasion of lung cancer cells co-cultured with fibroblasts |
title_full | A 3D culture system for evaluating the combined effects of cisplatin and anti-fibrotic drugs on the growth and invasion of lung cancer cells co-cultured with fibroblasts |
title_fullStr | A 3D culture system for evaluating the combined effects of cisplatin and anti-fibrotic drugs on the growth and invasion of lung cancer cells co-cultured with fibroblasts |
title_full_unstemmed | A 3D culture system for evaluating the combined effects of cisplatin and anti-fibrotic drugs on the growth and invasion of lung cancer cells co-cultured with fibroblasts |
title_short | A 3D culture system for evaluating the combined effects of cisplatin and anti-fibrotic drugs on the growth and invasion of lung cancer cells co-cultured with fibroblasts |
title_sort | 3d culture system for evaluating the combined effects of cisplatin and anti-fibrotic drugs on the growth and invasion of lung cancer cells co-cultured with fibroblasts |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060027/ https://www.ncbi.nlm.nih.gov/pubmed/37006781 http://dx.doi.org/10.1063/5.0115464 |
work_keys_str_mv | AT panhueijyuan a3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT leechiawei a3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT wuliyu a3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT hsuhenghua a3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT tungyichung a3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT liaoweiyu a3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT leechauhwang a3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT panhueijyuan 3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT leechiawei 3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT wuliyu 3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT hsuhenghua 3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT tungyichung 3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT liaoweiyu 3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts AT leechauhwang 3dculturesystemforevaluatingthecombinedeffectsofcisplatinandantifibroticdrugsonthegrowthandinvasionoflungcancercellscoculturedwithfibroblasts |