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Susceptibility of the Intact and Traumatized Feline Cornea to In Vitro Binding and Invasion by Acanthamoeba castellanii

Acanthamoeba castellanii (A. castellanii) displays host specificity at the level of the ocular surface. This study determined the susceptibility of the intact and traumatized feline cornea to A. castellanii binding and invasion relative to other host species with established susceptibility and resis...

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Autores principales: Ledbetter, Eric C., Dong, Longying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cornea 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060048/
https://www.ncbi.nlm.nih.gov/pubmed/36518074
http://dx.doi.org/10.1097/ICO.0000000000003220
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author Ledbetter, Eric C.
Dong, Longying
author_facet Ledbetter, Eric C.
Dong, Longying
author_sort Ledbetter, Eric C.
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description Acanthamoeba castellanii (A. castellanii) displays host specificity at the level of the ocular surface. This study determined the susceptibility of the intact and traumatized feline cornea to A. castellanii binding and invasion relative to other host species with established susceptibility and resistance to Acanthamoeba binding. METHODS: Full-thickness buttons of fresh feline, porcine, and canine corneas were prepared. The corneal epithelium was confirmed intact by fluorescein staining or lightly scarified with a 25-G needle to simulate corneal trauma. Acanthamoeba castellanii was axenically cultivated. Corneal buttons were incubated with the parasite suspension or parasite-free medium for 18 hours at 35°C. Corneal buttons were rinsed, fixed, and processed for histopathology and immunohistochemistry using immunoperoxidase and immunofluorescence methods of amoeba detection. RESULTS: Numerous amoebae were bound to feline and porcine corneas incubated with parasites. In both intact and traumatized corneas, amoebae were detected at all levels in the corneal epithelium and within the anterior stroma. In traumatized corneal sections, amoebae were frequently present in regions of epithelial damage. Corneal architecture was well-preserved in sections incubated with parasite-free medium; however, epithelial cell sloughing, separation of epithelial layers, and epithelial detachment from the stroma were observed in corneas incubated with amoebae. Intact and traumatized canine corneas were relatively free of adherent amoebae, and corneal architecture was indistinguishable between sections incubated with the parasite suspension and parasite-free medium. CONCLUSIONS: The feline cornea is highly susceptible to in vitro binding and invasion by A. castellanii. Acanthamoeba binding to the feline cornea does not require a previous epithelial defect.
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spelling pubmed-100600482023-03-30 Susceptibility of the Intact and Traumatized Feline Cornea to In Vitro Binding and Invasion by Acanthamoeba castellanii Ledbetter, Eric C. Dong, Longying Cornea Basic Investigation Acanthamoeba castellanii (A. castellanii) displays host specificity at the level of the ocular surface. This study determined the susceptibility of the intact and traumatized feline cornea to A. castellanii binding and invasion relative to other host species with established susceptibility and resistance to Acanthamoeba binding. METHODS: Full-thickness buttons of fresh feline, porcine, and canine corneas were prepared. The corneal epithelium was confirmed intact by fluorescein staining or lightly scarified with a 25-G needle to simulate corneal trauma. Acanthamoeba castellanii was axenically cultivated. Corneal buttons were incubated with the parasite suspension or parasite-free medium for 18 hours at 35°C. Corneal buttons were rinsed, fixed, and processed for histopathology and immunohistochemistry using immunoperoxidase and immunofluorescence methods of amoeba detection. RESULTS: Numerous amoebae were bound to feline and porcine corneas incubated with parasites. In both intact and traumatized corneas, amoebae were detected at all levels in the corneal epithelium and within the anterior stroma. In traumatized corneal sections, amoebae were frequently present in regions of epithelial damage. Corneal architecture was well-preserved in sections incubated with parasite-free medium; however, epithelial cell sloughing, separation of epithelial layers, and epithelial detachment from the stroma were observed in corneas incubated with amoebae. Intact and traumatized canine corneas were relatively free of adherent amoebae, and corneal architecture was indistinguishable between sections incubated with the parasite suspension and parasite-free medium. CONCLUSIONS: The feline cornea is highly susceptible to in vitro binding and invasion by A. castellanii. Acanthamoeba binding to the feline cornea does not require a previous epithelial defect. Cornea 2023-05 2022-12-13 /pmc/articles/PMC10060048/ /pubmed/36518074 http://dx.doi.org/10.1097/ICO.0000000000003220 Text en Copyright © 2022 The Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
spellingShingle Basic Investigation
Ledbetter, Eric C.
Dong, Longying
Susceptibility of the Intact and Traumatized Feline Cornea to In Vitro Binding and Invasion by Acanthamoeba castellanii
title Susceptibility of the Intact and Traumatized Feline Cornea to In Vitro Binding and Invasion by Acanthamoeba castellanii
title_full Susceptibility of the Intact and Traumatized Feline Cornea to In Vitro Binding and Invasion by Acanthamoeba castellanii
title_fullStr Susceptibility of the Intact and Traumatized Feline Cornea to In Vitro Binding and Invasion by Acanthamoeba castellanii
title_full_unstemmed Susceptibility of the Intact and Traumatized Feline Cornea to In Vitro Binding and Invasion by Acanthamoeba castellanii
title_short Susceptibility of the Intact and Traumatized Feline Cornea to In Vitro Binding and Invasion by Acanthamoeba castellanii
title_sort susceptibility of the intact and traumatized feline cornea to in vitro binding and invasion by acanthamoeba castellanii
topic Basic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060048/
https://www.ncbi.nlm.nih.gov/pubmed/36518074
http://dx.doi.org/10.1097/ICO.0000000000003220
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