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Possible contribution of phosphate to the pathogenesis of chronic kidney disease in dolphins

This study aimed to investigate whether phosphate contributes to the pathogenesis of chronic kidney disease (CKD) in dolphins. Renal necropsy tissue of an aged captive dolphin was analyzed and in vitro experiments using cultured immortalized dolphin proximal tubular (DolKT-1) cells were performed. A...

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Autores principales: Jahan, Nourin, Ohsaki, Hiroyuki, Kaneko, Kiyoko, Rahman, Asadur, Nishiyama, Takeshi, Koizumi, Makoto, Yamanaka, Shuichiro, Kitada, Kento, Sugiura, Yuki, Matsui, Kenji, Yokoo, Takashi, Hamano, Takayuki, Kuro-o, Makoto, Itou, Takuya, Suzuki, Miwa, Ueda, Keiichi, Nishiyama, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060237/
https://www.ncbi.nlm.nih.gov/pubmed/36991108
http://dx.doi.org/10.1038/s41598-023-32399-6
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author Jahan, Nourin
Ohsaki, Hiroyuki
Kaneko, Kiyoko
Rahman, Asadur
Nishiyama, Takeshi
Koizumi, Makoto
Yamanaka, Shuichiro
Kitada, Kento
Sugiura, Yuki
Matsui, Kenji
Yokoo, Takashi
Hamano, Takayuki
Kuro-o, Makoto
Itou, Takuya
Suzuki, Miwa
Ueda, Keiichi
Nishiyama, Akira
author_facet Jahan, Nourin
Ohsaki, Hiroyuki
Kaneko, Kiyoko
Rahman, Asadur
Nishiyama, Takeshi
Koizumi, Makoto
Yamanaka, Shuichiro
Kitada, Kento
Sugiura, Yuki
Matsui, Kenji
Yokoo, Takashi
Hamano, Takayuki
Kuro-o, Makoto
Itou, Takuya
Suzuki, Miwa
Ueda, Keiichi
Nishiyama, Akira
author_sort Jahan, Nourin
collection PubMed
description This study aimed to investigate whether phosphate contributes to the pathogenesis of chronic kidney disease (CKD) in dolphins. Renal necropsy tissue of an aged captive dolphin was analyzed and in vitro experiments using cultured immortalized dolphin proximal tubular (DolKT-1) cells were performed. An older dolphin in captivity died of myocarditis, but its renal function was within the normal range until shortly before death. In renal necropsy tissue, obvious glomerular and tubulointerstitial changes were not observed except for renal infarction resulting from myocarditis. However, a computed tomography scan showed medullary calcification in reniculi. Micro area X-ray diffractometry and infrared absorption spectrometry showed that the calcified areas were primarily composed of hydroxyapatite. In vitro experiments showed that treatment with both phosphate and calciprotein particles (CPPs) resulted in cell viability loss and lactate dehydrogenase release in DolKT-1 cells. However, treatment with magnesium markedly attenuated this cellular injury induced by phosphate, but not by CPPs. Magnesium dose-dependently decreased CPP formation. These data support the hypothesis that continuous exposure to high phosphate contributes to the progression of CKD in captive-aged dolphins. Our data also suggest that phosphate-induced renal injury is mediated by CPP formation in dolphins, and it is attenuated by magnesium administration.
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spelling pubmed-100602372023-03-31 Possible contribution of phosphate to the pathogenesis of chronic kidney disease in dolphins Jahan, Nourin Ohsaki, Hiroyuki Kaneko, Kiyoko Rahman, Asadur Nishiyama, Takeshi Koizumi, Makoto Yamanaka, Shuichiro Kitada, Kento Sugiura, Yuki Matsui, Kenji Yokoo, Takashi Hamano, Takayuki Kuro-o, Makoto Itou, Takuya Suzuki, Miwa Ueda, Keiichi Nishiyama, Akira Sci Rep Article This study aimed to investigate whether phosphate contributes to the pathogenesis of chronic kidney disease (CKD) in dolphins. Renal necropsy tissue of an aged captive dolphin was analyzed and in vitro experiments using cultured immortalized dolphin proximal tubular (DolKT-1) cells were performed. An older dolphin in captivity died of myocarditis, but its renal function was within the normal range until shortly before death. In renal necropsy tissue, obvious glomerular and tubulointerstitial changes were not observed except for renal infarction resulting from myocarditis. However, a computed tomography scan showed medullary calcification in reniculi. Micro area X-ray diffractometry and infrared absorption spectrometry showed that the calcified areas were primarily composed of hydroxyapatite. In vitro experiments showed that treatment with both phosphate and calciprotein particles (CPPs) resulted in cell viability loss and lactate dehydrogenase release in DolKT-1 cells. However, treatment with magnesium markedly attenuated this cellular injury induced by phosphate, but not by CPPs. Magnesium dose-dependently decreased CPP formation. These data support the hypothesis that continuous exposure to high phosphate contributes to the progression of CKD in captive-aged dolphins. Our data also suggest that phosphate-induced renal injury is mediated by CPP formation in dolphins, and it is attenuated by magnesium administration. Nature Publishing Group UK 2023-03-29 /pmc/articles/PMC10060237/ /pubmed/36991108 http://dx.doi.org/10.1038/s41598-023-32399-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jahan, Nourin
Ohsaki, Hiroyuki
Kaneko, Kiyoko
Rahman, Asadur
Nishiyama, Takeshi
Koizumi, Makoto
Yamanaka, Shuichiro
Kitada, Kento
Sugiura, Yuki
Matsui, Kenji
Yokoo, Takashi
Hamano, Takayuki
Kuro-o, Makoto
Itou, Takuya
Suzuki, Miwa
Ueda, Keiichi
Nishiyama, Akira
Possible contribution of phosphate to the pathogenesis of chronic kidney disease in dolphins
title Possible contribution of phosphate to the pathogenesis of chronic kidney disease in dolphins
title_full Possible contribution of phosphate to the pathogenesis of chronic kidney disease in dolphins
title_fullStr Possible contribution of phosphate to the pathogenesis of chronic kidney disease in dolphins
title_full_unstemmed Possible contribution of phosphate to the pathogenesis of chronic kidney disease in dolphins
title_short Possible contribution of phosphate to the pathogenesis of chronic kidney disease in dolphins
title_sort possible contribution of phosphate to the pathogenesis of chronic kidney disease in dolphins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060237/
https://www.ncbi.nlm.nih.gov/pubmed/36991108
http://dx.doi.org/10.1038/s41598-023-32399-6
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