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Prokineticin 1 is a novel factor regulating porcine corpus luteum function

Prokineticin 1 (PROK1) is a pleiotropic factor secreted by endocrine glands; however, its role has not been studied in the corpus luteum (CL) during pregnancy in any species. The present study aimed to investigate the contribution of PROK1 in regulating processes related to porcine CL function and r...

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Autores principales: Baryla, Monika, Goryszewska-Szczurek, Ewelina, Kaczynski, Piotr, Balboni, Gianfranco, Waclawik, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060428/
https://www.ncbi.nlm.nih.gov/pubmed/36991037
http://dx.doi.org/10.1038/s41598-023-32132-3
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author Baryla, Monika
Goryszewska-Szczurek, Ewelina
Kaczynski, Piotr
Balboni, Gianfranco
Waclawik, Agnieszka
author_facet Baryla, Monika
Goryszewska-Szczurek, Ewelina
Kaczynski, Piotr
Balboni, Gianfranco
Waclawik, Agnieszka
author_sort Baryla, Monika
collection PubMed
description Prokineticin 1 (PROK1) is a pleiotropic factor secreted by endocrine glands; however, its role has not been studied in the corpus luteum (CL) during pregnancy in any species. The present study aimed to investigate the contribution of PROK1 in regulating processes related to porcine CL function and regression: steroidogenesis, luteal cell apoptosis and viability, and angiogenesis. The luteal expression of PROK1 was greater on Days 12 and 14 of pregnancy compared to Day 9. PROK1 protein expression during pregnancy increased gradually and peaked on Day 14, when it was also significantly higher than that on Day 14 of the estrous cycle. Prokineticin receptor 1 (PROKR1) mRNA abundance increased on Days 12 and 14 of pregnancy, whereas PROKR2 elevated on Day 14 of the estrous cycle. PROK1, acting via PROKR1, stimulated the expression of genes involved in progesterone synthesis, as well as progesterone secretion by luteal tissue. PROK1–PROKR1 signaling reduced apoptosis and increased the viability of luteal cells. PROK1 acting through PROKR1 stimulated angiogenesis by increasing capillary-like structure formation by luteal endothelial cells and elevating angiogenin gene expression and VEGFA secretion by luteal tissue. Our results indicate that PROK1 regulates processes vital for maintaining luteal function during early pregnancy and the mid-luteal phase.
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spelling pubmed-100604282023-03-31 Prokineticin 1 is a novel factor regulating porcine corpus luteum function Baryla, Monika Goryszewska-Szczurek, Ewelina Kaczynski, Piotr Balboni, Gianfranco Waclawik, Agnieszka Sci Rep Article Prokineticin 1 (PROK1) is a pleiotropic factor secreted by endocrine glands; however, its role has not been studied in the corpus luteum (CL) during pregnancy in any species. The present study aimed to investigate the contribution of PROK1 in regulating processes related to porcine CL function and regression: steroidogenesis, luteal cell apoptosis and viability, and angiogenesis. The luteal expression of PROK1 was greater on Days 12 and 14 of pregnancy compared to Day 9. PROK1 protein expression during pregnancy increased gradually and peaked on Day 14, when it was also significantly higher than that on Day 14 of the estrous cycle. Prokineticin receptor 1 (PROKR1) mRNA abundance increased on Days 12 and 14 of pregnancy, whereas PROKR2 elevated on Day 14 of the estrous cycle. PROK1, acting via PROKR1, stimulated the expression of genes involved in progesterone synthesis, as well as progesterone secretion by luteal tissue. PROK1–PROKR1 signaling reduced apoptosis and increased the viability of luteal cells. PROK1 acting through PROKR1 stimulated angiogenesis by increasing capillary-like structure formation by luteal endothelial cells and elevating angiogenin gene expression and VEGFA secretion by luteal tissue. Our results indicate that PROK1 regulates processes vital for maintaining luteal function during early pregnancy and the mid-luteal phase. Nature Publishing Group UK 2023-03-29 /pmc/articles/PMC10060428/ /pubmed/36991037 http://dx.doi.org/10.1038/s41598-023-32132-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Baryla, Monika
Goryszewska-Szczurek, Ewelina
Kaczynski, Piotr
Balboni, Gianfranco
Waclawik, Agnieszka
Prokineticin 1 is a novel factor regulating porcine corpus luteum function
title Prokineticin 1 is a novel factor regulating porcine corpus luteum function
title_full Prokineticin 1 is a novel factor regulating porcine corpus luteum function
title_fullStr Prokineticin 1 is a novel factor regulating porcine corpus luteum function
title_full_unstemmed Prokineticin 1 is a novel factor regulating porcine corpus luteum function
title_short Prokineticin 1 is a novel factor regulating porcine corpus luteum function
title_sort prokineticin 1 is a novel factor regulating porcine corpus luteum function
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060428/
https://www.ncbi.nlm.nih.gov/pubmed/36991037
http://dx.doi.org/10.1038/s41598-023-32132-3
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