Cargando…

Metabolome profile variations in common bean (Phaseolus vulgaris L.) resistant and susceptible genotypes incited by rust (Uromyces appendiculatus)

The causal agent of rust, Uromyces appendiculatus is a major constraint for common bean (Phaseolus vulgaris) production. This pathogen causes substantial yield losses in many common bean production areas worldwide. U. appendiculatus is widely distributed and although there have been numerous breakth...

Descripción completa

Detalles Bibliográficos
Autores principales: Makhumbila, Penny, Rauwane, Molemi E., Muedi, Hangwani H., Madala, Ntakadzeni E., Figlan, Sandiswa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060544/
https://www.ncbi.nlm.nih.gov/pubmed/37007949
http://dx.doi.org/10.3389/fgene.2023.1141201
_version_ 1785017114584678400
author Makhumbila, Penny
Rauwane, Molemi E.
Muedi, Hangwani H.
Madala, Ntakadzeni E.
Figlan, Sandiswa
author_facet Makhumbila, Penny
Rauwane, Molemi E.
Muedi, Hangwani H.
Madala, Ntakadzeni E.
Figlan, Sandiswa
author_sort Makhumbila, Penny
collection PubMed
description The causal agent of rust, Uromyces appendiculatus is a major constraint for common bean (Phaseolus vulgaris) production. This pathogen causes substantial yield losses in many common bean production areas worldwide. U. appendiculatus is widely distributed and although there have been numerous breakthroughs in breeding for resistance, its ability to mutate and evolve still poses a major threat to common bean production. An understanding of plant phytochemical properties can aid in accelerating breeding for rust resistance. In this study, metabolome profiles of two common bean genotypes Teebus-RR-1 (resistant) and Golden Gate Wax (susceptible) were investigated for their response to U. appendiculatus races (1 and 3) at 14- and 21-days post-infection (dpi) using liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-qTOF-MS). Non-targeted data analysis revealed 71 known metabolites that were putatively annotated, and a total of 33 were statistically significant. Key metabolites including flavonoids, terpenoids, alkaloids and lipids were found to be incited by rust infections in both genotypes. Resistant genotype as compared to the susceptible genotype differentially enriched metabolites including aconifine, D-sucrose, galangin, rutarin and others as a defence mechanism against the rust pathogen. The results suggest that timely response to pathogen attack by signalling the production of specific metabolites can be used as a strategy to understand plant defence. This is the first study to illustrate the utilization of metabolomics to understand the interaction of common bean with rust.
format Online
Article
Text
id pubmed-10060544
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-100605442023-03-31 Metabolome profile variations in common bean (Phaseolus vulgaris L.) resistant and susceptible genotypes incited by rust (Uromyces appendiculatus) Makhumbila, Penny Rauwane, Molemi E. Muedi, Hangwani H. Madala, Ntakadzeni E. Figlan, Sandiswa Front Genet Genetics The causal agent of rust, Uromyces appendiculatus is a major constraint for common bean (Phaseolus vulgaris) production. This pathogen causes substantial yield losses in many common bean production areas worldwide. U. appendiculatus is widely distributed and although there have been numerous breakthroughs in breeding for resistance, its ability to mutate and evolve still poses a major threat to common bean production. An understanding of plant phytochemical properties can aid in accelerating breeding for rust resistance. In this study, metabolome profiles of two common bean genotypes Teebus-RR-1 (resistant) and Golden Gate Wax (susceptible) were investigated for their response to U. appendiculatus races (1 and 3) at 14- and 21-days post-infection (dpi) using liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-qTOF-MS). Non-targeted data analysis revealed 71 known metabolites that were putatively annotated, and a total of 33 were statistically significant. Key metabolites including flavonoids, terpenoids, alkaloids and lipids were found to be incited by rust infections in both genotypes. Resistant genotype as compared to the susceptible genotype differentially enriched metabolites including aconifine, D-sucrose, galangin, rutarin and others as a defence mechanism against the rust pathogen. The results suggest that timely response to pathogen attack by signalling the production of specific metabolites can be used as a strategy to understand plant defence. This is the first study to illustrate the utilization of metabolomics to understand the interaction of common bean with rust. Frontiers Media S.A. 2023-03-16 /pmc/articles/PMC10060544/ /pubmed/37007949 http://dx.doi.org/10.3389/fgene.2023.1141201 Text en Copyright © 2023 Makhumbila, Rauwane, Muedi, Madala and Figlan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Makhumbila, Penny
Rauwane, Molemi E.
Muedi, Hangwani H.
Madala, Ntakadzeni E.
Figlan, Sandiswa
Metabolome profile variations in common bean (Phaseolus vulgaris L.) resistant and susceptible genotypes incited by rust (Uromyces appendiculatus)
title Metabolome profile variations in common bean (Phaseolus vulgaris L.) resistant and susceptible genotypes incited by rust (Uromyces appendiculatus)
title_full Metabolome profile variations in common bean (Phaseolus vulgaris L.) resistant and susceptible genotypes incited by rust (Uromyces appendiculatus)
title_fullStr Metabolome profile variations in common bean (Phaseolus vulgaris L.) resistant and susceptible genotypes incited by rust (Uromyces appendiculatus)
title_full_unstemmed Metabolome profile variations in common bean (Phaseolus vulgaris L.) resistant and susceptible genotypes incited by rust (Uromyces appendiculatus)
title_short Metabolome profile variations in common bean (Phaseolus vulgaris L.) resistant and susceptible genotypes incited by rust (Uromyces appendiculatus)
title_sort metabolome profile variations in common bean (phaseolus vulgaris l.) resistant and susceptible genotypes incited by rust (uromyces appendiculatus)
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060544/
https://www.ncbi.nlm.nih.gov/pubmed/37007949
http://dx.doi.org/10.3389/fgene.2023.1141201
work_keys_str_mv AT makhumbilapenny metabolomeprofilevariationsincommonbeanphaseolusvulgarislresistantandsusceptiblegenotypesincitedbyrusturomycesappendiculatus
AT rauwanemolemie metabolomeprofilevariationsincommonbeanphaseolusvulgarislresistantandsusceptiblegenotypesincitedbyrusturomycesappendiculatus
AT muedihangwanih metabolomeprofilevariationsincommonbeanphaseolusvulgarislresistantandsusceptiblegenotypesincitedbyrusturomycesappendiculatus
AT madalantakadzenie metabolomeprofilevariationsincommonbeanphaseolusvulgarislresistantandsusceptiblegenotypesincitedbyrusturomycesappendiculatus
AT figlansandiswa metabolomeprofilevariationsincommonbeanphaseolusvulgarislresistantandsusceptiblegenotypesincitedbyrusturomycesappendiculatus