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A multi-color video-ophthalmoscopes allows to measure the spectral distribution of light absorption of blood in the human retina
Based on our previously developed mono-color video-ophthalmoscope a multi-color video-ophthalmoscope was developed. Using narrow band transmission filters, this instrument allows to measure the pulsatile cardiac cycle induced blood volume changes in the human retina for any wavelength in the sensiti...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060599/ https://www.ncbi.nlm.nih.gov/pubmed/37007778 http://dx.doi.org/10.3389/fmed.2023.1125154 |
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author | Tornow, Ralf-Peter Odstrcilik, Jan Kolar, Radim |
author_facet | Tornow, Ralf-Peter Odstrcilik, Jan Kolar, Radim |
author_sort | Tornow, Ralf-Peter |
collection | PubMed |
description | Based on our previously developed mono-color video-ophthalmoscope a multi-color video-ophthalmoscope was developed. Using narrow band transmission filters, this instrument allows to measure the pulsatile cardiac cycle induced blood volume changes in the human retina for any wavelength in the sensitivity range of the used CMOS-camera. In this key experiment, video sequences (8 s, 25 fps, 200 frames) of the optic nerve head (ONH) were acquire for seven wavelengths between 475 nm and 677 nm one after the other. After image registration of all frames of each video sequence (to compensate for eye movements) and trend correction (to compensate for slow intensity changes), the amplitude of the cardiac cycle induced light intensity changes (pulsatile absorption amplitude PAA) can be calculated for all seven wavelengths. The results confirmed that the spectral distribution of PAA (λ) follows the distribution of the light absorption of blood. The measured values correspond to the absorption of a thin blood layer of about 0.5 μm thickness. |
format | Online Article Text |
id | pubmed-10060599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100605992023-03-31 A multi-color video-ophthalmoscopes allows to measure the spectral distribution of light absorption of blood in the human retina Tornow, Ralf-Peter Odstrcilik, Jan Kolar, Radim Front Med (Lausanne) Medicine Based on our previously developed mono-color video-ophthalmoscope a multi-color video-ophthalmoscope was developed. Using narrow band transmission filters, this instrument allows to measure the pulsatile cardiac cycle induced blood volume changes in the human retina for any wavelength in the sensitivity range of the used CMOS-camera. In this key experiment, video sequences (8 s, 25 fps, 200 frames) of the optic nerve head (ONH) were acquire for seven wavelengths between 475 nm and 677 nm one after the other. After image registration of all frames of each video sequence (to compensate for eye movements) and trend correction (to compensate for slow intensity changes), the amplitude of the cardiac cycle induced light intensity changes (pulsatile absorption amplitude PAA) can be calculated for all seven wavelengths. The results confirmed that the spectral distribution of PAA (λ) follows the distribution of the light absorption of blood. The measured values correspond to the absorption of a thin blood layer of about 0.5 μm thickness. Frontiers Media S.A. 2023-03-16 /pmc/articles/PMC10060599/ /pubmed/37007778 http://dx.doi.org/10.3389/fmed.2023.1125154 Text en Copyright © 2023 Tornow, Odstrcilik and Kolar. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medicine Tornow, Ralf-Peter Odstrcilik, Jan Kolar, Radim A multi-color video-ophthalmoscopes allows to measure the spectral distribution of light absorption of blood in the human retina |
title | A multi-color video-ophthalmoscopes allows to measure the spectral distribution of light absorption of blood in the human retina |
title_full | A multi-color video-ophthalmoscopes allows to measure the spectral distribution of light absorption of blood in the human retina |
title_fullStr | A multi-color video-ophthalmoscopes allows to measure the spectral distribution of light absorption of blood in the human retina |
title_full_unstemmed | A multi-color video-ophthalmoscopes allows to measure the spectral distribution of light absorption of blood in the human retina |
title_short | A multi-color video-ophthalmoscopes allows to measure the spectral distribution of light absorption of blood in the human retina |
title_sort | multi-color video-ophthalmoscopes allows to measure the spectral distribution of light absorption of blood in the human retina |
topic | Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060599/ https://www.ncbi.nlm.nih.gov/pubmed/37007778 http://dx.doi.org/10.3389/fmed.2023.1125154 |
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