Cargando…
Production of copropophyrin III, biliverdin and bilirubin by the rufomycin producer, Streptomyces atratus
Heme is best known for its role as a versatile prosthetic group in prokaryotic and eukaryotic proteins with diverse biological functions including gas and electron transport, as well as a wide array of redox chemistry. However, free heme and related tetrapyrroles also have important roles in the cel...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060970/ https://www.ncbi.nlm.nih.gov/pubmed/37007481 http://dx.doi.org/10.3389/fmicb.2023.1092166 |
_version_ | 1785017198393163776 |
---|---|
author | Perez-Ortiz, Gustavo Sidda, John D. Peate, Jessica Ciccarelli, Davide Ding, Yaoyu Barry, Sarah M. |
author_facet | Perez-Ortiz, Gustavo Sidda, John D. Peate, Jessica Ciccarelli, Davide Ding, Yaoyu Barry, Sarah M. |
author_sort | Perez-Ortiz, Gustavo |
collection | PubMed |
description | Heme is best known for its role as a versatile prosthetic group in prokaryotic and eukaryotic proteins with diverse biological functions including gas and electron transport, as well as a wide array of redox chemistry. However, free heme and related tetrapyrroles also have important roles in the cell. In several bacterial strains, heme biosynthetic precursors and degradation products have been proposed to function as signaling molecules, ion chelators, antioxidants and photoprotectants. While the uptake and degradation of heme by bacterial pathogens is well studied, less is understood about the physiological role of these processes and their products in non-pathogenic bacteria. Streptomyces are slow growing soil bacteria known for their extraordinary capacity to produce complex secondary metabolites, particularly many clinically used antibiotics. Here we report the unambiguous identification of three tetrapyrrole metabolites from heme metabolism, coproporphyrin III, biliverdin and bilirubin, in culture extracts of the rufomycin antibiotic producing Streptomyces atratus DSM41673. We propose that biliverdin and bilirubin may combat oxidative stress induced by nitric oxide production during rufomycin biosynthesis, and indicate the genes involved in their production. This is, to our knowledge, the first report of the production of all three of these tetrapyrroles by a Streptomycete. |
format | Online Article Text |
id | pubmed-10060970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100609702023-03-31 Production of copropophyrin III, biliverdin and bilirubin by the rufomycin producer, Streptomyces atratus Perez-Ortiz, Gustavo Sidda, John D. Peate, Jessica Ciccarelli, Davide Ding, Yaoyu Barry, Sarah M. Front Microbiol Microbiology Heme is best known for its role as a versatile prosthetic group in prokaryotic and eukaryotic proteins with diverse biological functions including gas and electron transport, as well as a wide array of redox chemistry. However, free heme and related tetrapyrroles also have important roles in the cell. In several bacterial strains, heme biosynthetic precursors and degradation products have been proposed to function as signaling molecules, ion chelators, antioxidants and photoprotectants. While the uptake and degradation of heme by bacterial pathogens is well studied, less is understood about the physiological role of these processes and their products in non-pathogenic bacteria. Streptomyces are slow growing soil bacteria known for their extraordinary capacity to produce complex secondary metabolites, particularly many clinically used antibiotics. Here we report the unambiguous identification of three tetrapyrrole metabolites from heme metabolism, coproporphyrin III, biliverdin and bilirubin, in culture extracts of the rufomycin antibiotic producing Streptomyces atratus DSM41673. We propose that biliverdin and bilirubin may combat oxidative stress induced by nitric oxide production during rufomycin biosynthesis, and indicate the genes involved in their production. This is, to our knowledge, the first report of the production of all three of these tetrapyrroles by a Streptomycete. Frontiers Media S.A. 2023-03-16 /pmc/articles/PMC10060970/ /pubmed/37007481 http://dx.doi.org/10.3389/fmicb.2023.1092166 Text en Copyright © 2023 Perez-Ortiz, Sidda, Peate, Ciccarelli, Ding and Barry. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Perez-Ortiz, Gustavo Sidda, John D. Peate, Jessica Ciccarelli, Davide Ding, Yaoyu Barry, Sarah M. Production of copropophyrin III, biliverdin and bilirubin by the rufomycin producer, Streptomyces atratus |
title | Production of copropophyrin III, biliverdin and bilirubin by the rufomycin producer, Streptomyces atratus |
title_full | Production of copropophyrin III, biliverdin and bilirubin by the rufomycin producer, Streptomyces atratus |
title_fullStr | Production of copropophyrin III, biliverdin and bilirubin by the rufomycin producer, Streptomyces atratus |
title_full_unstemmed | Production of copropophyrin III, biliverdin and bilirubin by the rufomycin producer, Streptomyces atratus |
title_short | Production of copropophyrin III, biliverdin and bilirubin by the rufomycin producer, Streptomyces atratus |
title_sort | production of copropophyrin iii, biliverdin and bilirubin by the rufomycin producer, streptomyces atratus |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060970/ https://www.ncbi.nlm.nih.gov/pubmed/37007481 http://dx.doi.org/10.3389/fmicb.2023.1092166 |
work_keys_str_mv | AT perezortizgustavo productionofcopropophyriniiibiliverdinandbilirubinbytherufomycinproducerstreptomycesatratus AT siddajohnd productionofcopropophyriniiibiliverdinandbilirubinbytherufomycinproducerstreptomycesatratus AT peatejessica productionofcopropophyriniiibiliverdinandbilirubinbytherufomycinproducerstreptomycesatratus AT ciccarellidavide productionofcopropophyriniiibiliverdinandbilirubinbytherufomycinproducerstreptomycesatratus AT dingyaoyu productionofcopropophyriniiibiliverdinandbilirubinbytherufomycinproducerstreptomycesatratus AT barrysarahm productionofcopropophyriniiibiliverdinandbilirubinbytherufomycinproducerstreptomycesatratus |