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A comparative study on various cell sources for constructing tissue-engineered meniscus

Injury to the meniscus is a common occurrence in the knee joint and its management remains a significant challenge in the clinic. Appropriate cell source is essential to cell-based tissue regeneration and cell therapy. Herein, three commonly used cell sources, namely, bone marrow mesenchymal stem ce...

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Autores principales: Zheng, Rui, Song, Daiying, Ding, Yangfan, Sun, Binbin, Lu, Changrui, Mo, Xiumei, Xu, Hui, Liu, Yu, Wu, Jinglei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061001/
https://www.ncbi.nlm.nih.gov/pubmed/37008037
http://dx.doi.org/10.3389/fbioe.2023.1128762
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author Zheng, Rui
Song, Daiying
Ding, Yangfan
Sun, Binbin
Lu, Changrui
Mo, Xiumei
Xu, Hui
Liu, Yu
Wu, Jinglei
author_facet Zheng, Rui
Song, Daiying
Ding, Yangfan
Sun, Binbin
Lu, Changrui
Mo, Xiumei
Xu, Hui
Liu, Yu
Wu, Jinglei
author_sort Zheng, Rui
collection PubMed
description Injury to the meniscus is a common occurrence in the knee joint and its management remains a significant challenge in the clinic. Appropriate cell source is essential to cell-based tissue regeneration and cell therapy. Herein, three commonly used cell sources, namely, bone marrow mesenchymal stem cell (BMSC), adipose-derived stem cell (ADSC), and articular chondrocyte, were comparatively evaluated to determine their potential for engineered meniscus tissue in the absence of growth factor stimulus. Cells were seeded on electrospun nanofiber yarn scaffolds that share similar aligned fibrous configurations with native meniscus tissue for constructing meniscus tissue in vitro. Our results show that cells proliferated robustly along nanofiber yarns to form organized cell-scaffold constructs, which recapitulate the typical circumferential fiber bundles of native meniscus. Chondrocytes exhibited different proliferative characteristics and formed engineered tissues with distinct biochemical and biomechanical properties compared to BMSC and ADSC. Chondrocytes maintained good chondrogenesis gene expression profiles and produced significantly increased chondrogenic matrix and form mature cartilage-like tissue as revealed by typical cartilage lacunae. In contrast, stem cells underwent predominately fibroblastic differentiation and generated greater collagen, which contributes to improved tensile strengths of cell-scaffold constructs in comparison to the chondrocyte. ADSC showed greater proliferative activity and increased collagen production than BMSC. These findings indicate that chondrocytes are superior to stem cells for constructing chondrogenic tissues while the latter is feasible to form fibroblastic tissue. Combination of chondrocytes and stem cells might be a possible solution to construct fibrocartilage tissue and meniscus repair and regeneration.
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spelling pubmed-100610012023-03-31 A comparative study on various cell sources for constructing tissue-engineered meniscus Zheng, Rui Song, Daiying Ding, Yangfan Sun, Binbin Lu, Changrui Mo, Xiumei Xu, Hui Liu, Yu Wu, Jinglei Front Bioeng Biotechnol Bioengineering and Biotechnology Injury to the meniscus is a common occurrence in the knee joint and its management remains a significant challenge in the clinic. Appropriate cell source is essential to cell-based tissue regeneration and cell therapy. Herein, three commonly used cell sources, namely, bone marrow mesenchymal stem cell (BMSC), adipose-derived stem cell (ADSC), and articular chondrocyte, were comparatively evaluated to determine their potential for engineered meniscus tissue in the absence of growth factor stimulus. Cells were seeded on electrospun nanofiber yarn scaffolds that share similar aligned fibrous configurations with native meniscus tissue for constructing meniscus tissue in vitro. Our results show that cells proliferated robustly along nanofiber yarns to form organized cell-scaffold constructs, which recapitulate the typical circumferential fiber bundles of native meniscus. Chondrocytes exhibited different proliferative characteristics and formed engineered tissues with distinct biochemical and biomechanical properties compared to BMSC and ADSC. Chondrocytes maintained good chondrogenesis gene expression profiles and produced significantly increased chondrogenic matrix and form mature cartilage-like tissue as revealed by typical cartilage lacunae. In contrast, stem cells underwent predominately fibroblastic differentiation and generated greater collagen, which contributes to improved tensile strengths of cell-scaffold constructs in comparison to the chondrocyte. ADSC showed greater proliferative activity and increased collagen production than BMSC. These findings indicate that chondrocytes are superior to stem cells for constructing chondrogenic tissues while the latter is feasible to form fibroblastic tissue. Combination of chondrocytes and stem cells might be a possible solution to construct fibrocartilage tissue and meniscus repair and regeneration. Frontiers Media S.A. 2023-03-16 /pmc/articles/PMC10061001/ /pubmed/37008037 http://dx.doi.org/10.3389/fbioe.2023.1128762 Text en Copyright © 2023 Zheng, Song, Ding, Sun, Lu, Mo, Xu, Liu and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Zheng, Rui
Song, Daiying
Ding, Yangfan
Sun, Binbin
Lu, Changrui
Mo, Xiumei
Xu, Hui
Liu, Yu
Wu, Jinglei
A comparative study on various cell sources for constructing tissue-engineered meniscus
title A comparative study on various cell sources for constructing tissue-engineered meniscus
title_full A comparative study on various cell sources for constructing tissue-engineered meniscus
title_fullStr A comparative study on various cell sources for constructing tissue-engineered meniscus
title_full_unstemmed A comparative study on various cell sources for constructing tissue-engineered meniscus
title_short A comparative study on various cell sources for constructing tissue-engineered meniscus
title_sort comparative study on various cell sources for constructing tissue-engineered meniscus
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061001/
https://www.ncbi.nlm.nih.gov/pubmed/37008037
http://dx.doi.org/10.3389/fbioe.2023.1128762
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