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The eNOS isoform exhibits increased expression and activation in the main olfactory bulb of nNOS knock-out mice

The main olfactory bulb (MOB) is a neural structure that processes olfactory information. Among the neurotransmitters present in the MOB, nitric oxide (NO) is particularly relevant as it performs a wide variety of functions. In this structure, NO is produced mainly by neuronal nitric oxide synthase...

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Autores principales: Pérez-Boyero, David, Hernández-Pérez, Carlos, Valero, Jorge, Cabedo, Valeria Lorena, Alonso, José Ramón, Díaz, David, Weruaga, Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061100/
https://www.ncbi.nlm.nih.gov/pubmed/37006472
http://dx.doi.org/10.3389/fncel.2023.1120836
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author Pérez-Boyero, David
Hernández-Pérez, Carlos
Valero, Jorge
Cabedo, Valeria Lorena
Alonso, José Ramón
Díaz, David
Weruaga, Eduardo
author_facet Pérez-Boyero, David
Hernández-Pérez, Carlos
Valero, Jorge
Cabedo, Valeria Lorena
Alonso, José Ramón
Díaz, David
Weruaga, Eduardo
author_sort Pérez-Boyero, David
collection PubMed
description The main olfactory bulb (MOB) is a neural structure that processes olfactory information. Among the neurotransmitters present in the MOB, nitric oxide (NO) is particularly relevant as it performs a wide variety of functions. In this structure, NO is produced mainly by neuronal nitric oxide synthase (nNOS) but also by inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS). The MOB is considered a region with great plasticity and the different NOS also show great plasticity. Therefore, it could be considered that this plasticity could compensate for various dysfunctional and pathological alterations. We examined the possible plasticity of iNOS and eNOS in the MOB in the absence of nNOS. For this, wild-type and nNOS knock-out (nNOS-KO) mice were used. We assessed whether the absence of nNOS expression could affect the olfactory capacity of mice, followed by the analysis of the expression and distribution of the NOS isoforms using qPCR and immunofluorescence. NO production in MOB was examined using both the Griess and histochemical NADPH-diaphorase reactions. The results indicate nNOS-KO mice have reduced olfactory capacity. We observed that in the nNOS-KO animal, there is an increase both in the expression of eNOS and NADPH-diaphorase, but no apparent change in the level of NO generated in the MOB. It can be concluded that the level of eNOS in the MOB of nNOS-KO is related to the maintenance of normal levels of NO. Therefore, our findings suggest that nNOS could be essential for the proper functioning of the olfactory system.
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spelling pubmed-100611002023-03-31 The eNOS isoform exhibits increased expression and activation in the main olfactory bulb of nNOS knock-out mice Pérez-Boyero, David Hernández-Pérez, Carlos Valero, Jorge Cabedo, Valeria Lorena Alonso, José Ramón Díaz, David Weruaga, Eduardo Front Cell Neurosci Neuroscience The main olfactory bulb (MOB) is a neural structure that processes olfactory information. Among the neurotransmitters present in the MOB, nitric oxide (NO) is particularly relevant as it performs a wide variety of functions. In this structure, NO is produced mainly by neuronal nitric oxide synthase (nNOS) but also by inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS). The MOB is considered a region with great plasticity and the different NOS also show great plasticity. Therefore, it could be considered that this plasticity could compensate for various dysfunctional and pathological alterations. We examined the possible plasticity of iNOS and eNOS in the MOB in the absence of nNOS. For this, wild-type and nNOS knock-out (nNOS-KO) mice were used. We assessed whether the absence of nNOS expression could affect the olfactory capacity of mice, followed by the analysis of the expression and distribution of the NOS isoforms using qPCR and immunofluorescence. NO production in MOB was examined using both the Griess and histochemical NADPH-diaphorase reactions. The results indicate nNOS-KO mice have reduced olfactory capacity. We observed that in the nNOS-KO animal, there is an increase both in the expression of eNOS and NADPH-diaphorase, but no apparent change in the level of NO generated in the MOB. It can be concluded that the level of eNOS in the MOB of nNOS-KO is related to the maintenance of normal levels of NO. Therefore, our findings suggest that nNOS could be essential for the proper functioning of the olfactory system. Frontiers Media S.A. 2023-03-16 /pmc/articles/PMC10061100/ /pubmed/37006472 http://dx.doi.org/10.3389/fncel.2023.1120836 Text en Copyright © 2023 Pérez-Boyero, Hernández-Pérez, Valero, Cabedo, Alonso, Díaz and Weruaga. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Pérez-Boyero, David
Hernández-Pérez, Carlos
Valero, Jorge
Cabedo, Valeria Lorena
Alonso, José Ramón
Díaz, David
Weruaga, Eduardo
The eNOS isoform exhibits increased expression and activation in the main olfactory bulb of nNOS knock-out mice
title The eNOS isoform exhibits increased expression and activation in the main olfactory bulb of nNOS knock-out mice
title_full The eNOS isoform exhibits increased expression and activation in the main olfactory bulb of nNOS knock-out mice
title_fullStr The eNOS isoform exhibits increased expression and activation in the main olfactory bulb of nNOS knock-out mice
title_full_unstemmed The eNOS isoform exhibits increased expression and activation in the main olfactory bulb of nNOS knock-out mice
title_short The eNOS isoform exhibits increased expression and activation in the main olfactory bulb of nNOS knock-out mice
title_sort enos isoform exhibits increased expression and activation in the main olfactory bulb of nnos knock-out mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061100/
https://www.ncbi.nlm.nih.gov/pubmed/37006472
http://dx.doi.org/10.3389/fncel.2023.1120836
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