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Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line
The presence of mobile genetic elements in Salmonella isolated from a chicken farm constitutes a potential risk for the appearance of emerging bacteria present in the food industry. These elements contribute to increased pathogenicity and antimicrobial resistance through genes that are related to th...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061128/ https://www.ncbi.nlm.nih.gov/pubmed/37007466 http://dx.doi.org/10.3389/fmicb.2023.1072793 |
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author | Krüger, Gabriel I. Pardo-Esté, Coral Zepeda, Phillippi Olivares-Pacheco, Jorge Galleguillos, Nicolas Suarez, Marcia Castro-Severyn, Juan Alvarez-Thon, Luis Tello, Mario Valdes, Jorge H. Saavedra, Claudia P. |
author_facet | Krüger, Gabriel I. Pardo-Esté, Coral Zepeda, Phillippi Olivares-Pacheco, Jorge Galleguillos, Nicolas Suarez, Marcia Castro-Severyn, Juan Alvarez-Thon, Luis Tello, Mario Valdes, Jorge H. Saavedra, Claudia P. |
author_sort | Krüger, Gabriel I. |
collection | PubMed |
description | The presence of mobile genetic elements in Salmonella isolated from a chicken farm constitutes a potential risk for the appearance of emerging bacteria present in the food industry. These elements contribute to increased pathogenicity and antimicrobial resistance through genes that are related to the formation of biofilms and resistance genes contained in plasmids, integrons, and transposons. One hundred and thirty-three Salmonella isolates from different stages of the production line, such as feed manufacturing, hatchery, broiler farm, poultry farm, and slaughterhouse, were identified, serotyped and sequenced. The most predominant serotype was Salmonella Infantis. Phylogenetic analyses demonstrated that the diversity and spread of strains in the pipeline are serotype-independent, and that isolates belonging to the same serotype are very closely related genetically. On the other hand, Salmonella Infantis isolates carried the pESI IncFIB plasmid harboring a wide variety of resistance genes, all linked to mobile genetic elements, and among carriers of these plasmids, the antibiograms showed differences in resistance profiles and this linked to a variety in plasmid structure, similarly observed in the diversity of Salmonella Heidelberg isolates carrying the IncI1-Iα plasmid. Mobile genetic elements encoding resistance and virulence genes also contributed to the differences in gene content. Antibiotic resistance genotypes were matched closely by the resistance phenotypes, with high frequency of tetracycline, aminoglycosides, and cephalosporins resistance. In conclusion, the contamination in the poultry industry is described throughout the entire production line, with mobile genetic elements leading to multi-drug resistant bacteria, thus promoting survival when challenged with various antimicrobial compounds. |
format | Online Article Text |
id | pubmed-10061128 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100611282023-03-31 Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line Krüger, Gabriel I. Pardo-Esté, Coral Zepeda, Phillippi Olivares-Pacheco, Jorge Galleguillos, Nicolas Suarez, Marcia Castro-Severyn, Juan Alvarez-Thon, Luis Tello, Mario Valdes, Jorge H. Saavedra, Claudia P. Front Microbiol Microbiology The presence of mobile genetic elements in Salmonella isolated from a chicken farm constitutes a potential risk for the appearance of emerging bacteria present in the food industry. These elements contribute to increased pathogenicity and antimicrobial resistance through genes that are related to the formation of biofilms and resistance genes contained in plasmids, integrons, and transposons. One hundred and thirty-three Salmonella isolates from different stages of the production line, such as feed manufacturing, hatchery, broiler farm, poultry farm, and slaughterhouse, were identified, serotyped and sequenced. The most predominant serotype was Salmonella Infantis. Phylogenetic analyses demonstrated that the diversity and spread of strains in the pipeline are serotype-independent, and that isolates belonging to the same serotype are very closely related genetically. On the other hand, Salmonella Infantis isolates carried the pESI IncFIB plasmid harboring a wide variety of resistance genes, all linked to mobile genetic elements, and among carriers of these plasmids, the antibiograms showed differences in resistance profiles and this linked to a variety in plasmid structure, similarly observed in the diversity of Salmonella Heidelberg isolates carrying the IncI1-Iα plasmid. Mobile genetic elements encoding resistance and virulence genes also contributed to the differences in gene content. Antibiotic resistance genotypes were matched closely by the resistance phenotypes, with high frequency of tetracycline, aminoglycosides, and cephalosporins resistance. In conclusion, the contamination in the poultry industry is described throughout the entire production line, with mobile genetic elements leading to multi-drug resistant bacteria, thus promoting survival when challenged with various antimicrobial compounds. Frontiers Media S.A. 2023-03-16 /pmc/articles/PMC10061128/ /pubmed/37007466 http://dx.doi.org/10.3389/fmicb.2023.1072793 Text en Copyright © 2023 Krüger, Pardo-Esté, Zepeda, Olivares-Pacheco, Galleguillos, Suarez, Castro-Severyn, Alvarez-Thon, Tello, Valdes and Saavedra. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Krüger, Gabriel I. Pardo-Esté, Coral Zepeda, Phillippi Olivares-Pacheco, Jorge Galleguillos, Nicolas Suarez, Marcia Castro-Severyn, Juan Alvarez-Thon, Luis Tello, Mario Valdes, Jorge H. Saavedra, Claudia P. Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line |
title | Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line |
title_full | Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line |
title_fullStr | Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line |
title_full_unstemmed | Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line |
title_short | Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line |
title_sort | mobile genetic elements drive the multidrug resistance and spread of salmonella serotypes along a poultry meat production line |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061128/ https://www.ncbi.nlm.nih.gov/pubmed/37007466 http://dx.doi.org/10.3389/fmicb.2023.1072793 |
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