Cargando…

Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line

The presence of mobile genetic elements in Salmonella isolated from a chicken farm constitutes a potential risk for the appearance of emerging bacteria present in the food industry. These elements contribute to increased pathogenicity and antimicrobial resistance through genes that are related to th...

Descripción completa

Detalles Bibliográficos
Autores principales: Krüger, Gabriel I., Pardo-Esté, Coral, Zepeda, Phillippi, Olivares-Pacheco, Jorge, Galleguillos, Nicolas, Suarez, Marcia, Castro-Severyn, Juan, Alvarez-Thon, Luis, Tello, Mario, Valdes, Jorge H., Saavedra, Claudia P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061128/
https://www.ncbi.nlm.nih.gov/pubmed/37007466
http://dx.doi.org/10.3389/fmicb.2023.1072793
_version_ 1785017231784017920
author Krüger, Gabriel I.
Pardo-Esté, Coral
Zepeda, Phillippi
Olivares-Pacheco, Jorge
Galleguillos, Nicolas
Suarez, Marcia
Castro-Severyn, Juan
Alvarez-Thon, Luis
Tello, Mario
Valdes, Jorge H.
Saavedra, Claudia P.
author_facet Krüger, Gabriel I.
Pardo-Esté, Coral
Zepeda, Phillippi
Olivares-Pacheco, Jorge
Galleguillos, Nicolas
Suarez, Marcia
Castro-Severyn, Juan
Alvarez-Thon, Luis
Tello, Mario
Valdes, Jorge H.
Saavedra, Claudia P.
author_sort Krüger, Gabriel I.
collection PubMed
description The presence of mobile genetic elements in Salmonella isolated from a chicken farm constitutes a potential risk for the appearance of emerging bacteria present in the food industry. These elements contribute to increased pathogenicity and antimicrobial resistance through genes that are related to the formation of biofilms and resistance genes contained in plasmids, integrons, and transposons. One hundred and thirty-three Salmonella isolates from different stages of the production line, such as feed manufacturing, hatchery, broiler farm, poultry farm, and slaughterhouse, were identified, serotyped and sequenced. The most predominant serotype was Salmonella Infantis. Phylogenetic analyses demonstrated that the diversity and spread of strains in the pipeline are serotype-independent, and that isolates belonging to the same serotype are very closely related genetically. On the other hand, Salmonella Infantis isolates carried the pESI IncFIB plasmid harboring a wide variety of resistance genes, all linked to mobile genetic elements, and among carriers of these plasmids, the antibiograms showed differences in resistance profiles and this linked to a variety in plasmid structure, similarly observed in the diversity of Salmonella Heidelberg isolates carrying the IncI1-Iα plasmid. Mobile genetic elements encoding resistance and virulence genes also contributed to the differences in gene content. Antibiotic resistance genotypes were matched closely by the resistance phenotypes, with high frequency of tetracycline, aminoglycosides, and cephalosporins resistance. In conclusion, the contamination in the poultry industry is described throughout the entire production line, with mobile genetic elements leading to multi-drug resistant bacteria, thus promoting survival when challenged with various antimicrobial compounds.
format Online
Article
Text
id pubmed-10061128
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-100611282023-03-31 Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line Krüger, Gabriel I. Pardo-Esté, Coral Zepeda, Phillippi Olivares-Pacheco, Jorge Galleguillos, Nicolas Suarez, Marcia Castro-Severyn, Juan Alvarez-Thon, Luis Tello, Mario Valdes, Jorge H. Saavedra, Claudia P. Front Microbiol Microbiology The presence of mobile genetic elements in Salmonella isolated from a chicken farm constitutes a potential risk for the appearance of emerging bacteria present in the food industry. These elements contribute to increased pathogenicity and antimicrobial resistance through genes that are related to the formation of biofilms and resistance genes contained in plasmids, integrons, and transposons. One hundred and thirty-three Salmonella isolates from different stages of the production line, such as feed manufacturing, hatchery, broiler farm, poultry farm, and slaughterhouse, were identified, serotyped and sequenced. The most predominant serotype was Salmonella Infantis. Phylogenetic analyses demonstrated that the diversity and spread of strains in the pipeline are serotype-independent, and that isolates belonging to the same serotype are very closely related genetically. On the other hand, Salmonella Infantis isolates carried the pESI IncFIB plasmid harboring a wide variety of resistance genes, all linked to mobile genetic elements, and among carriers of these plasmids, the antibiograms showed differences in resistance profiles and this linked to a variety in plasmid structure, similarly observed in the diversity of Salmonella Heidelberg isolates carrying the IncI1-Iα plasmid. Mobile genetic elements encoding resistance and virulence genes also contributed to the differences in gene content. Antibiotic resistance genotypes were matched closely by the resistance phenotypes, with high frequency of tetracycline, aminoglycosides, and cephalosporins resistance. In conclusion, the contamination in the poultry industry is described throughout the entire production line, with mobile genetic elements leading to multi-drug resistant bacteria, thus promoting survival when challenged with various antimicrobial compounds. Frontiers Media S.A. 2023-03-16 /pmc/articles/PMC10061128/ /pubmed/37007466 http://dx.doi.org/10.3389/fmicb.2023.1072793 Text en Copyright © 2023 Krüger, Pardo-Esté, Zepeda, Olivares-Pacheco, Galleguillos, Suarez, Castro-Severyn, Alvarez-Thon, Tello, Valdes and Saavedra. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Krüger, Gabriel I.
Pardo-Esté, Coral
Zepeda, Phillippi
Olivares-Pacheco, Jorge
Galleguillos, Nicolas
Suarez, Marcia
Castro-Severyn, Juan
Alvarez-Thon, Luis
Tello, Mario
Valdes, Jorge H.
Saavedra, Claudia P.
Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line
title Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line
title_full Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line
title_fullStr Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line
title_full_unstemmed Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line
title_short Mobile genetic elements drive the multidrug resistance and spread of Salmonella serotypes along a poultry meat production line
title_sort mobile genetic elements drive the multidrug resistance and spread of salmonella serotypes along a poultry meat production line
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061128/
https://www.ncbi.nlm.nih.gov/pubmed/37007466
http://dx.doi.org/10.3389/fmicb.2023.1072793
work_keys_str_mv AT krugergabrieli mobilegeneticelementsdrivethemultidrugresistanceandspreadofsalmonellaserotypesalongapoultrymeatproductionline
AT pardoestecoral mobilegeneticelementsdrivethemultidrugresistanceandspreadofsalmonellaserotypesalongapoultrymeatproductionline
AT zepedaphillippi mobilegeneticelementsdrivethemultidrugresistanceandspreadofsalmonellaserotypesalongapoultrymeatproductionline
AT olivarespachecojorge mobilegeneticelementsdrivethemultidrugresistanceandspreadofsalmonellaserotypesalongapoultrymeatproductionline
AT galleguillosnicolas mobilegeneticelementsdrivethemultidrugresistanceandspreadofsalmonellaserotypesalongapoultrymeatproductionline
AT suarezmarcia mobilegeneticelementsdrivethemultidrugresistanceandspreadofsalmonellaserotypesalongapoultrymeatproductionline
AT castroseverynjuan mobilegeneticelementsdrivethemultidrugresistanceandspreadofsalmonellaserotypesalongapoultrymeatproductionline
AT alvarezthonluis mobilegeneticelementsdrivethemultidrugresistanceandspreadofsalmonellaserotypesalongapoultrymeatproductionline
AT tellomario mobilegeneticelementsdrivethemultidrugresistanceandspreadofsalmonellaserotypesalongapoultrymeatproductionline
AT valdesjorgeh mobilegeneticelementsdrivethemultidrugresistanceandspreadofsalmonellaserotypesalongapoultrymeatproductionline
AT saavedraclaudiap mobilegeneticelementsdrivethemultidrugresistanceandspreadofsalmonellaserotypesalongapoultrymeatproductionline