E-Cigarette Liquids and Aldehyde Flavoring Agents Inhibit CYP2A6 Activity in Lung Epithelial Cells

[Image: see text] Certain e-liquids and aromatic aldehyde flavoring agents were previously identified as inhibitors of microsomal recombinant CYP2A6, the primary nicotine-metabolizing enzyme. However, due to their reactive nature, aldehydes may react with cellular components before reaching CYP2A6 i...

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Autores principales: Winters, Brett R., Clapp, Phillip W., Simmons, Steven O., Kochar, Tavleen K., Jaspers, Ilona, Madden, Michael C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061538/
https://www.ncbi.nlm.nih.gov/pubmed/37008141
http://dx.doi.org/10.1021/acsomega.2c08258
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author Winters, Brett R.
Clapp, Phillip W.
Simmons, Steven O.
Kochar, Tavleen K.
Jaspers, Ilona
Madden, Michael C.
author_facet Winters, Brett R.
Clapp, Phillip W.
Simmons, Steven O.
Kochar, Tavleen K.
Jaspers, Ilona
Madden, Michael C.
author_sort Winters, Brett R.
collection PubMed
description [Image: see text] Certain e-liquids and aromatic aldehyde flavoring agents were previously identified as inhibitors of microsomal recombinant CYP2A6, the primary nicotine-metabolizing enzyme. However, due to their reactive nature, aldehydes may react with cellular components before reaching CYP2A6 in the endoplasmic reticulum. To determine whether e-liquid flavoring agents inhibited CYP2A6 in a cellular system, we investigated their effects on CYP2A6 using BEAS-2B cells transduced to overexpress CYP2A6. We demonstrated that two e-liquids and three aldehyde flavoring agents (cinnamaldehyde, benzaldehyde, and ethyl vanillin) exhibited dose-dependent inhibition of cellular CYP2A6.
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spelling pubmed-100615382023-03-31 E-Cigarette Liquids and Aldehyde Flavoring Agents Inhibit CYP2A6 Activity in Lung Epithelial Cells Winters, Brett R. Clapp, Phillip W. Simmons, Steven O. Kochar, Tavleen K. Jaspers, Ilona Madden, Michael C. ACS Omega [Image: see text] Certain e-liquids and aromatic aldehyde flavoring agents were previously identified as inhibitors of microsomal recombinant CYP2A6, the primary nicotine-metabolizing enzyme. However, due to their reactive nature, aldehydes may react with cellular components before reaching CYP2A6 in the endoplasmic reticulum. To determine whether e-liquid flavoring agents inhibited CYP2A6 in a cellular system, we investigated their effects on CYP2A6 using BEAS-2B cells transduced to overexpress CYP2A6. We demonstrated that two e-liquids and three aldehyde flavoring agents (cinnamaldehyde, benzaldehyde, and ethyl vanillin) exhibited dose-dependent inhibition of cellular CYP2A6. American Chemical Society 2023-03-13 /pmc/articles/PMC10061538/ /pubmed/37008141 http://dx.doi.org/10.1021/acsomega.2c08258 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Winters, Brett R.
Clapp, Phillip W.
Simmons, Steven O.
Kochar, Tavleen K.
Jaspers, Ilona
Madden, Michael C.
E-Cigarette Liquids and Aldehyde Flavoring Agents Inhibit CYP2A6 Activity in Lung Epithelial Cells
title E-Cigarette Liquids and Aldehyde Flavoring Agents Inhibit CYP2A6 Activity in Lung Epithelial Cells
title_full E-Cigarette Liquids and Aldehyde Flavoring Agents Inhibit CYP2A6 Activity in Lung Epithelial Cells
title_fullStr E-Cigarette Liquids and Aldehyde Flavoring Agents Inhibit CYP2A6 Activity in Lung Epithelial Cells
title_full_unstemmed E-Cigarette Liquids and Aldehyde Flavoring Agents Inhibit CYP2A6 Activity in Lung Epithelial Cells
title_short E-Cigarette Liquids and Aldehyde Flavoring Agents Inhibit CYP2A6 Activity in Lung Epithelial Cells
title_sort e-cigarette liquids and aldehyde flavoring agents inhibit cyp2a6 activity in lung epithelial cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10061538/
https://www.ncbi.nlm.nih.gov/pubmed/37008141
http://dx.doi.org/10.1021/acsomega.2c08258
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