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Comparison of McMaster and Mini-FLOTAC techniques for the diagnosis of internal parasites in pigs

This purpose of this study was to compare the efficiency of the McMaster and Mini-FLOTAC quantitative techniques in the investigation of helminths in feces of pigs. An analysis was made of 74 fecal samples from pigs raised on family farms located in Rio de Janeiro, Brazil. These were analyzed by the...

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Autores principales: Class, Camila Souza Carvalho, Fialho, Priscila Alves, Alves, Leucio Câmara, Silveira, Renato Luiz, Amendoeira, Maria Regina Reis, Knackfuss, Fabiana Batalha, Barbosa, Alynne da Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Colégio Brasileiro de Parasitologia Veterinária 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10062010/
https://www.ncbi.nlm.nih.gov/pubmed/36995837
http://dx.doi.org/10.1590/S1984-29612023013
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author Class, Camila Souza Carvalho
Fialho, Priscila Alves
Alves, Leucio Câmara
Silveira, Renato Luiz
Amendoeira, Maria Regina Reis
Knackfuss, Fabiana Batalha
Barbosa, Alynne da Silva
author_facet Class, Camila Souza Carvalho
Fialho, Priscila Alves
Alves, Leucio Câmara
Silveira, Renato Luiz
Amendoeira, Maria Regina Reis
Knackfuss, Fabiana Batalha
Barbosa, Alynne da Silva
author_sort Class, Camila Souza Carvalho
collection PubMed
description This purpose of this study was to compare the efficiency of the McMaster and Mini-FLOTAC quantitative techniques in the investigation of helminths in feces of pigs. An analysis was made of 74 fecal samples from pigs raised on family farms located in Rio de Janeiro, Brazil. These were analyzed by the Mini-FLOTAC and McMaster techniques in a solution of 1,200g/mL NaCl. This investigation revealed a superiority in the frequency of all helminths detected by Mini-FLOTAC, including Ascaris suum, Trichuris suis, strongyles and Strongyloides ransomi. The Kappa index revealed substantial agreement in all comparisons made in relation to the frequency of positive samples. However, significant statistical differences in the comparison of EPGs between McMaster and Mini-FLOTAC were observed for all nematodes (p ≤0.05). Higher values of Pearson’s linear correlation coefficient (r), between the techniques in relation to EPG were observed for A. suum and T. suis, differently from what was observed for strongyles and S. ransomi. Mini-FLOTAC proved to be a more satisfactory and reliable technique both for the diagnosis of parasites and for the determination of EPG in pig feces due to the larger size of its counting chambers, thus increasing the helminth egg recovery rates.
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spelling pubmed-100620102023-03-31 Comparison of McMaster and Mini-FLOTAC techniques for the diagnosis of internal parasites in pigs Class, Camila Souza Carvalho Fialho, Priscila Alves Alves, Leucio Câmara Silveira, Renato Luiz Amendoeira, Maria Regina Reis Knackfuss, Fabiana Batalha Barbosa, Alynne da Silva Rev Bras Parasitol Vet Short Communication This purpose of this study was to compare the efficiency of the McMaster and Mini-FLOTAC quantitative techniques in the investigation of helminths in feces of pigs. An analysis was made of 74 fecal samples from pigs raised on family farms located in Rio de Janeiro, Brazil. These were analyzed by the Mini-FLOTAC and McMaster techniques in a solution of 1,200g/mL NaCl. This investigation revealed a superiority in the frequency of all helminths detected by Mini-FLOTAC, including Ascaris suum, Trichuris suis, strongyles and Strongyloides ransomi. The Kappa index revealed substantial agreement in all comparisons made in relation to the frequency of positive samples. However, significant statistical differences in the comparison of EPGs between McMaster and Mini-FLOTAC were observed for all nematodes (p ≤0.05). Higher values of Pearson’s linear correlation coefficient (r), between the techniques in relation to EPG were observed for A. suum and T. suis, differently from what was observed for strongyles and S. ransomi. Mini-FLOTAC proved to be a more satisfactory and reliable technique both for the diagnosis of parasites and for the determination of EPG in pig feces due to the larger size of its counting chambers, thus increasing the helminth egg recovery rates. Colégio Brasileiro de Parasitologia Veterinária 2023-03-27 /pmc/articles/PMC10062010/ /pubmed/36995837 http://dx.doi.org/10.1590/S1984-29612023013 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Class, Camila Souza Carvalho
Fialho, Priscila Alves
Alves, Leucio Câmara
Silveira, Renato Luiz
Amendoeira, Maria Regina Reis
Knackfuss, Fabiana Batalha
Barbosa, Alynne da Silva
Comparison of McMaster and Mini-FLOTAC techniques for the diagnosis of internal parasites in pigs
title Comparison of McMaster and Mini-FLOTAC techniques for the diagnosis of internal parasites in pigs
title_full Comparison of McMaster and Mini-FLOTAC techniques for the diagnosis of internal parasites in pigs
title_fullStr Comparison of McMaster and Mini-FLOTAC techniques for the diagnosis of internal parasites in pigs
title_full_unstemmed Comparison of McMaster and Mini-FLOTAC techniques for the diagnosis of internal parasites in pigs
title_short Comparison of McMaster and Mini-FLOTAC techniques for the diagnosis of internal parasites in pigs
title_sort comparison of mcmaster and mini-flotac techniques for the diagnosis of internal parasites in pigs
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10062010/
https://www.ncbi.nlm.nih.gov/pubmed/36995837
http://dx.doi.org/10.1590/S1984-29612023013
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