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Design, Synthesis, and Evaluation of Peptides Derived from L1 Protein Against Bovine Papillomavirus-1/2 Identified Along Mexico’s Cattle Export Route
INTRODUCTION: Bovine papillomatosis affects animal health and represents one of the greatest economic losses in the livestock sector. New control and prevention methods to protect the livestock industry from this disease are necessary. The aim of this study was to evaluate a candidate peptide for an...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sciendo
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10062047/ https://www.ncbi.nlm.nih.gov/pubmed/37008764 http://dx.doi.org/10.2478/jvetres-2023-0003 |
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author | García Coronado, Paola Leonor Romo Sáenz, César Iván Kawas, Jorge R. Zarate Triviño, Diana Ginette Ramos Zayas, Yareellys Elena Santana Krímskaya, Silvia Rodríguez Padilla, Cristina Armides Franco Molina, Moisés |
author_facet | García Coronado, Paola Leonor Romo Sáenz, César Iván Kawas, Jorge R. Zarate Triviño, Diana Ginette Ramos Zayas, Yareellys Elena Santana Krímskaya, Silvia Rodríguez Padilla, Cristina Armides Franco Molina, Moisés |
author_sort | García Coronado, Paola Leonor |
collection | PubMed |
description | INTRODUCTION: Bovine papillomatosis affects animal health and represents one of the greatest economic losses in the livestock sector. New control and prevention methods to protect the livestock industry from this disease are necessary. The aim of this study was to evaluate a candidate peptide for antibody production against bovine papillomavirus (BPV). MATERIAL AND METHODS: A total of 64 cattle underwent wart excision among 5,485 cattle distributed over 2 to 4 farms per state and 12 farms in total in the four Mexican states of Tabasco, Chiapas, Veracruz, and Nuevo León. The prevalence of bovine papillomatosis per farm was calculated by wart visualisation. The warts were genotyped by PCR and sequenced, then a phylogenetic tree was built using MEGA X software. A synthetic peptide was designed in the ABCpred, Bepipred 2.0, Bepipred IDBT, Bepitope, LBtope, and MHC II predictor online server software’s based on the C-terminal region of the L1 protein. Mice antibody production was induced by subcutaneous immunisation with 50 μg of synthetic peptide and evaluated by indirect ELISA. RESULTS: The prevalence of BPV was higher in Tabasco, Chiapas, and Veracruz. Bovine papillomaviruses 1 and 2 were found in all representative samples. A phylogenetic tree showed that Mexican sequences were located in exclusive clades yet were highly related to international ones. The peptide immunisation induced antibody titres of 1 : 10,000/1 : 1,000,000 against synthetic peptide and whole wart lysate (WWL), respectively. CONCLUSION: Co-infections of BPV-1 and -2 were found in all four states. Immunisation of BALB/C mice with BPV-1/2–derived synthetic peptide based on the C-terminal region of the major viral capsid protein L1 induced the production of specific antibodies able to recognise BPV-1/2 viral particles from bovine WWL. |
format | Online Article Text |
id | pubmed-10062047 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-100620472023-03-31 Design, Synthesis, and Evaluation of Peptides Derived from L1 Protein Against Bovine Papillomavirus-1/2 Identified Along Mexico’s Cattle Export Route García Coronado, Paola Leonor Romo Sáenz, César Iván Kawas, Jorge R. Zarate Triviño, Diana Ginette Ramos Zayas, Yareellys Elena Santana Krímskaya, Silvia Rodríguez Padilla, Cristina Armides Franco Molina, Moisés J Vet Res Research Articles INTRODUCTION: Bovine papillomatosis affects animal health and represents one of the greatest economic losses in the livestock sector. New control and prevention methods to protect the livestock industry from this disease are necessary. The aim of this study was to evaluate a candidate peptide for antibody production against bovine papillomavirus (BPV). MATERIAL AND METHODS: A total of 64 cattle underwent wart excision among 5,485 cattle distributed over 2 to 4 farms per state and 12 farms in total in the four Mexican states of Tabasco, Chiapas, Veracruz, and Nuevo León. The prevalence of bovine papillomatosis per farm was calculated by wart visualisation. The warts were genotyped by PCR and sequenced, then a phylogenetic tree was built using MEGA X software. A synthetic peptide was designed in the ABCpred, Bepipred 2.0, Bepipred IDBT, Bepitope, LBtope, and MHC II predictor online server software’s based on the C-terminal region of the L1 protein. Mice antibody production was induced by subcutaneous immunisation with 50 μg of synthetic peptide and evaluated by indirect ELISA. RESULTS: The prevalence of BPV was higher in Tabasco, Chiapas, and Veracruz. Bovine papillomaviruses 1 and 2 were found in all representative samples. A phylogenetic tree showed that Mexican sequences were located in exclusive clades yet were highly related to international ones. The peptide immunisation induced antibody titres of 1 : 10,000/1 : 1,000,000 against synthetic peptide and whole wart lysate (WWL), respectively. CONCLUSION: Co-infections of BPV-1 and -2 were found in all four states. Immunisation of BALB/C mice with BPV-1/2–derived synthetic peptide based on the C-terminal region of the major viral capsid protein L1 induced the production of specific antibodies able to recognise BPV-1/2 viral particles from bovine WWL. Sciendo 2023-02-28 /pmc/articles/PMC10062047/ /pubmed/37008764 http://dx.doi.org/10.2478/jvetres-2023-0003 Text en © 2023 P.L. García Coronado et al., published by Sciendo https://creativecommons.org/licenses/by-nc-nd/3.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. |
spellingShingle | Research Articles García Coronado, Paola Leonor Romo Sáenz, César Iván Kawas, Jorge R. Zarate Triviño, Diana Ginette Ramos Zayas, Yareellys Elena Santana Krímskaya, Silvia Rodríguez Padilla, Cristina Armides Franco Molina, Moisés Design, Synthesis, and Evaluation of Peptides Derived from L1 Protein Against Bovine Papillomavirus-1/2 Identified Along Mexico’s Cattle Export Route |
title | Design, Synthesis, and Evaluation of Peptides Derived from L1 Protein Against Bovine Papillomavirus-1/2 Identified Along Mexico’s Cattle Export Route |
title_full | Design, Synthesis, and Evaluation of Peptides Derived from L1 Protein Against Bovine Papillomavirus-1/2 Identified Along Mexico’s Cattle Export Route |
title_fullStr | Design, Synthesis, and Evaluation of Peptides Derived from L1 Protein Against Bovine Papillomavirus-1/2 Identified Along Mexico’s Cattle Export Route |
title_full_unstemmed | Design, Synthesis, and Evaluation of Peptides Derived from L1 Protein Against Bovine Papillomavirus-1/2 Identified Along Mexico’s Cattle Export Route |
title_short | Design, Synthesis, and Evaluation of Peptides Derived from L1 Protein Against Bovine Papillomavirus-1/2 Identified Along Mexico’s Cattle Export Route |
title_sort | design, synthesis, and evaluation of peptides derived from l1 protein against bovine papillomavirus-1/2 identified along mexico’s cattle export route |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10062047/ https://www.ncbi.nlm.nih.gov/pubmed/37008764 http://dx.doi.org/10.2478/jvetres-2023-0003 |
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