The impact of dental metal restorations on the oral oxidative stress level

BACKGROUND: Dental materials may influence the equilibrium between production and destruction of free radicals, thus creating conditions for developing of local or general oxidative stress. Metal ions, emitted from base dental alloys, may cause changes in cell structures and functions. Isoprostane concentration may indicate possible cell damage, caused by free radicals, and can be used for evaluation of the oxidative stress level. The aim of this study was to compare the level of 8-isoPGF2-alpha in saliva in patients with and without metal dental restorations. MATERIAL AND METHODS: 35 patients were divided in two groups according to the presence or absence of metal dental objects. Non-stimulated and stimulated saliva samples were collected. The concentration of 8-isoPGF2-alpha was measured by liquid chromatography tandem mass spectrometry. For statistical analysis non-parametric Mann-Whitney test, Kruskal Wallis test, and Wilcoxon signed-rank test were applied. RESULTS: There was a significant difference in the concentration of 8-isoPGF2-alpha between the samples of non-stimulated and stimulated saliva. The concentration of 8-isoPGF2-alpha in non-stimulated saliva in patients with metal dental restorations was significantly higher than the one in the group of patients without metal objects. CONCLUSIONS: The presence of metal dental restorations increases the concentration of 8-isoPGF2-alpha in non-stimulated saliva. Key words:Saliva, dental metal restorations, oxidative stress.