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Physicochemical characterization of porcine respiratory aerosol and considerations for future aerovirology
Understanding the mechanisms which inactivate airborne viruses is a current challenge. The composition of human respiratory aerosol is poorly understood and needs to be adequately investigated for use in aerovirology studies. Here, the physicochemical properties of porcine respiratory fluid (PRF) fr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10063220/ https://www.ncbi.nlm.nih.gov/pubmed/37007717 http://dx.doi.org/10.1093/pnasnexus/pgad087 |
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author | Groth, Robert Niazi, Sadegh Spann, Kirsten Johnson, Graham R Ristovski, Zoran |
author_facet | Groth, Robert Niazi, Sadegh Spann, Kirsten Johnson, Graham R Ristovski, Zoran |
author_sort | Groth, Robert |
collection | PubMed |
description | Understanding the mechanisms which inactivate airborne viruses is a current challenge. The composition of human respiratory aerosol is poorly understood and needs to be adequately investigated for use in aerovirology studies. Here, the physicochemical properties of porcine respiratory fluid (PRF) from the trachea and lungs were investigated both in bulk solutions and in aerosols. The mass ratio of Na:K in PRF compared with cell culture media (Dulbecco's Modified Eagle Medium, DMEM), which is frequently used in aerovirology studies, was significantly lower (∼2:1 vs ∼16:1). PRF contained significantly more potassium and protein than DMEM. PRF aerosols of all samples were similarly hygroscopic to human respiratory aerosol. PRF particles could nucleate with spatially separated crystals, indicating that the protein matrix was sufficiently viscous to prevent the complete coalescence of aqueous salts prior to efflorescence. The effects of these differences in compositions on the viability of viruses are currently not well understood. The virus suspensions in aerovirology studies need to be reconsidered to adequately reflect a real-world expiration scenario. |
format | Online Article Text |
id | pubmed-10063220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-100632202023-03-31 Physicochemical characterization of porcine respiratory aerosol and considerations for future aerovirology Groth, Robert Niazi, Sadegh Spann, Kirsten Johnson, Graham R Ristovski, Zoran PNAS Nexus Biological, Health, and Medical Sciences Understanding the mechanisms which inactivate airborne viruses is a current challenge. The composition of human respiratory aerosol is poorly understood and needs to be adequately investigated for use in aerovirology studies. Here, the physicochemical properties of porcine respiratory fluid (PRF) from the trachea and lungs were investigated both in bulk solutions and in aerosols. The mass ratio of Na:K in PRF compared with cell culture media (Dulbecco's Modified Eagle Medium, DMEM), which is frequently used in aerovirology studies, was significantly lower (∼2:1 vs ∼16:1). PRF contained significantly more potassium and protein than DMEM. PRF aerosols of all samples were similarly hygroscopic to human respiratory aerosol. PRF particles could nucleate with spatially separated crystals, indicating that the protein matrix was sufficiently viscous to prevent the complete coalescence of aqueous salts prior to efflorescence. The effects of these differences in compositions on the viability of viruses are currently not well understood. The virus suspensions in aerovirology studies need to be reconsidered to adequately reflect a real-world expiration scenario. Oxford University Press 2023-03-22 /pmc/articles/PMC10063220/ /pubmed/37007717 http://dx.doi.org/10.1093/pnasnexus/pgad087 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of National Academy of Sciences. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Biological, Health, and Medical Sciences Groth, Robert Niazi, Sadegh Spann, Kirsten Johnson, Graham R Ristovski, Zoran Physicochemical characterization of porcine respiratory aerosol and considerations for future aerovirology |
title | Physicochemical characterization of porcine respiratory aerosol and considerations for future aerovirology |
title_full | Physicochemical characterization of porcine respiratory aerosol and considerations for future aerovirology |
title_fullStr | Physicochemical characterization of porcine respiratory aerosol and considerations for future aerovirology |
title_full_unstemmed | Physicochemical characterization of porcine respiratory aerosol and considerations for future aerovirology |
title_short | Physicochemical characterization of porcine respiratory aerosol and considerations for future aerovirology |
title_sort | physicochemical characterization of porcine respiratory aerosol and considerations for future aerovirology |
topic | Biological, Health, and Medical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10063220/ https://www.ncbi.nlm.nih.gov/pubmed/37007717 http://dx.doi.org/10.1093/pnasnexus/pgad087 |
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