Identification and genetic diversity of grapevine virus L in Greece

In this study, grapevine virus L (GVL) was identified for the first time in Greece through the application of high-throughput sequencing of total RNA from grapevine samples. Further investigation of the prevalence of GVL in Greek vineyards by RT-PCR revealed its presence in 5.5% (31/560) of the test...

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Detalles Bibliográficos
Autores principales: Panailidou, P., Galeou, A., Beris, D., Pappi, P., Theologidis, I., Tzagaki, E., Lotos, L., Varveri, C., Katis, N. I., Maliogka, V. I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2023
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10063476/
https://www.ncbi.nlm.nih.gov/pubmed/36995397
http://dx.doi.org/10.1007/s00705-023-05756-z
Descripción
Sumario:In this study, grapevine virus L (GVL) was identified for the first time in Greece through the application of high-throughput sequencing of total RNA from grapevine samples. Further investigation of the prevalence of GVL in Greek vineyards by RT-PCR revealed its presence in 5.5% (31/560) of the tested samples, which originated from six viticultural areas of the country. Comparative sequence analysis based on the CP gene revealed a high degree of genetic variability among GVL isolates, while phylogenetic analysis grouped the Greek isolates in three of the five phylogroups formed, with most of them being classified in phylogroup I. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-023-05756-z.

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