Identification and genetic diversity of grapevine virus L in Greece

In this study, grapevine virus L (GVL) was identified for the first time in Greece through the application of high-throughput sequencing of total RNA from grapevine samples. Further investigation of the prevalence of GVL in Greek vineyards by RT-PCR revealed its presence in 5.5% (31/560) of the test...

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Autores principales: Panailidou, P., Galeou, A., Beris, D., Pappi, P., Theologidis, I., Tzagaki, E., Lotos, L., Varveri, C., Katis, N. I., Maliogka, V. I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2023
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10063476/
https://www.ncbi.nlm.nih.gov/pubmed/36995397
http://dx.doi.org/10.1007/s00705-023-05756-z
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author Panailidou, P.
Galeou, A.
Beris, D.
Pappi, P.
Theologidis, I.
Tzagaki, E.
Lotos, L.
Varveri, C.
Katis, N. I.
Maliogka, V. I.
author_facet Panailidou, P.
Galeou, A.
Beris, D.
Pappi, P.
Theologidis, I.
Tzagaki, E.
Lotos, L.
Varveri, C.
Katis, N. I.
Maliogka, V. I.
author_sort Panailidou, P.
collection PubMed
description In this study, grapevine virus L (GVL) was identified for the first time in Greece through the application of high-throughput sequencing of total RNA from grapevine samples. Further investigation of the prevalence of GVL in Greek vineyards by RT-PCR revealed its presence in 5.5% (31/560) of the tested samples, which originated from six viticultural areas of the country. Comparative sequence analysis based on the CP gene revealed a high degree of genetic variability among GVL isolates, while phylogenetic analysis grouped the Greek isolates in three of the five phylogroups formed, with most of them being classified in phylogroup I. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-023-05756-z.
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spelling pubmed-100634762023-04-01 Identification and genetic diversity of grapevine virus L in Greece Panailidou, P. Galeou, A. Beris, D. Pappi, P. Theologidis, I. Tzagaki, E. Lotos, L. Varveri, C. Katis, N. I. Maliogka, V. I. Arch Virol Brief Report In this study, grapevine virus L (GVL) was identified for the first time in Greece through the application of high-throughput sequencing of total RNA from grapevine samples. Further investigation of the prevalence of GVL in Greek vineyards by RT-PCR revealed its presence in 5.5% (31/560) of the tested samples, which originated from six viticultural areas of the country. Comparative sequence analysis based on the CP gene revealed a high degree of genetic variability among GVL isolates, while phylogenetic analysis grouped the Greek isolates in three of the five phylogroups formed, with most of them being classified in phylogroup I. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-023-05756-z. Springer Vienna 2023-03-30 2023 /pmc/articles/PMC10063476/ /pubmed/36995397 http://dx.doi.org/10.1007/s00705-023-05756-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Report
Panailidou, P.
Galeou, A.
Beris, D.
Pappi, P.
Theologidis, I.
Tzagaki, E.
Lotos, L.
Varveri, C.
Katis, N. I.
Maliogka, V. I.
Identification and genetic diversity of grapevine virus L in Greece
title Identification and genetic diversity of grapevine virus L in Greece
title_full Identification and genetic diversity of grapevine virus L in Greece
title_fullStr Identification and genetic diversity of grapevine virus L in Greece
title_full_unstemmed Identification and genetic diversity of grapevine virus L in Greece
title_short Identification and genetic diversity of grapevine virus L in Greece
title_sort identification and genetic diversity of grapevine virus l in greece
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10063476/
https://www.ncbi.nlm.nih.gov/pubmed/36995397
http://dx.doi.org/10.1007/s00705-023-05756-z
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