Cargando…
Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation
Introduction: It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice. However, the early dynamic process of the inflammatory response caused by lipopolysaccharide (LPS) and the anti-inflammatory effect of hydrogen has not been definitively...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10063881/ https://www.ncbi.nlm.nih.gov/pubmed/37007020 http://dx.doi.org/10.3389/fphar.2023.1138762 |
_version_ | 1785017787233599488 |
---|---|
author | Wei, Youzhen Wang, Kun Zhang, Yafang Duan, Yi Tian, Yan Yin, Hongling Fu, Xuelian Ma, Zuan Zhou, Jianjun Yu, Min Ni, Qingbin Tang, Wenjie |
author_facet | Wei, Youzhen Wang, Kun Zhang, Yafang Duan, Yi Tian, Yan Yin, Hongling Fu, Xuelian Ma, Zuan Zhou, Jianjun Yu, Min Ni, Qingbin Tang, Wenjie |
author_sort | Wei, Youzhen |
collection | PubMed |
description | Introduction: It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice. However, the early dynamic process of the inflammatory response caused by lipopolysaccharide (LPS) and the anti-inflammatory effect of hydrogen has not been definitively reported. Methods: Inflammation in male C57/BL6J mice or RAW264.7 cells was induced with LPS, for which hydrogen was immediately administered until samples were taken. Pathological changes in lung tissue were assessed using hematoxylin and eosin (HE) staining. Levels of inflammatory factors in serum were determined using liquid protein chip. The mRNA levels of chemotactic factors in lung tissues, leukocytes, and peritoneal macrophages were measured by qRT-PCR. The expression levels of IL-1α and HIF-1α were measured by immunocytochemistry. Results: Hydrogen alleviated LPS-induced inflammatory infiltration in the lung tissues of mice. Among the 23 inflammatory factors screened, LPS-induced upregulation of IL-1α etc. was significantly inhibited by hydrogen within 1 hour. The mRNA expression of MCP-1, MIP-1α, G-CSF, and RANTES was inhibited obviously by hydrogen at 0.5 and 1 h in mouse peritoneal macrophages. In addition, hydrogen significantly blocked LPS or H2O2-induced upregulation of HIF-1α, and IL-1α in 0.5 h in RAW264.7 cells. Discussion: The results suggested that hydrogen is potentially inhibitive against inflammation by inhibiting HIF-1α and IL-1α release at early occurrence. The target of the inhibitive LPS-induced-inflammatory action of hydrogen is chemokines in macrophages in the peritoneal cavity. This study provides direct experimental evidence for quickly controlling inflammation with the translational application of a hydrogen-assisted protocol. |
format | Online Article Text |
id | pubmed-10063881 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100638812023-04-01 Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation Wei, Youzhen Wang, Kun Zhang, Yafang Duan, Yi Tian, Yan Yin, Hongling Fu, Xuelian Ma, Zuan Zhou, Jianjun Yu, Min Ni, Qingbin Tang, Wenjie Front Pharmacol Pharmacology Introduction: It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice. However, the early dynamic process of the inflammatory response caused by lipopolysaccharide (LPS) and the anti-inflammatory effect of hydrogen has not been definitively reported. Methods: Inflammation in male C57/BL6J mice or RAW264.7 cells was induced with LPS, for which hydrogen was immediately administered until samples were taken. Pathological changes in lung tissue were assessed using hematoxylin and eosin (HE) staining. Levels of inflammatory factors in serum were determined using liquid protein chip. The mRNA levels of chemotactic factors in lung tissues, leukocytes, and peritoneal macrophages were measured by qRT-PCR. The expression levels of IL-1α and HIF-1α were measured by immunocytochemistry. Results: Hydrogen alleviated LPS-induced inflammatory infiltration in the lung tissues of mice. Among the 23 inflammatory factors screened, LPS-induced upregulation of IL-1α etc. was significantly inhibited by hydrogen within 1 hour. The mRNA expression of MCP-1, MIP-1α, G-CSF, and RANTES was inhibited obviously by hydrogen at 0.5 and 1 h in mouse peritoneal macrophages. In addition, hydrogen significantly blocked LPS or H2O2-induced upregulation of HIF-1α, and IL-1α in 0.5 h in RAW264.7 cells. Discussion: The results suggested that hydrogen is potentially inhibitive against inflammation by inhibiting HIF-1α and IL-1α release at early occurrence. The target of the inhibitive LPS-induced-inflammatory action of hydrogen is chemokines in macrophages in the peritoneal cavity. This study provides direct experimental evidence for quickly controlling inflammation with the translational application of a hydrogen-assisted protocol. Frontiers Media S.A. 2023-03-17 /pmc/articles/PMC10063881/ /pubmed/37007020 http://dx.doi.org/10.3389/fphar.2023.1138762 Text en Copyright © 2023 Wei, Wang, Zhang, Duan, Tian, Yin, Fu, Ma, Zhou, Yu, Ni and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Wei, Youzhen Wang, Kun Zhang, Yafang Duan, Yi Tian, Yan Yin, Hongling Fu, Xuelian Ma, Zuan Zhou, Jianjun Yu, Min Ni, Qingbin Tang, Wenjie Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation |
title | Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation |
title_full | Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation |
title_fullStr | Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation |
title_full_unstemmed | Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation |
title_short | Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation |
title_sort | potent anti-inflammatory responses: role of hydrogen in il-1α dominated early phase systemic inflammation |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10063881/ https://www.ncbi.nlm.nih.gov/pubmed/37007020 http://dx.doi.org/10.3389/fphar.2023.1138762 |
work_keys_str_mv | AT weiyouzhen potentantiinflammatoryresponsesroleofhydrogeninil1adominatedearlyphasesystemicinflammation AT wangkun potentantiinflammatoryresponsesroleofhydrogeninil1adominatedearlyphasesystemicinflammation AT zhangyafang potentantiinflammatoryresponsesroleofhydrogeninil1adominatedearlyphasesystemicinflammation AT duanyi potentantiinflammatoryresponsesroleofhydrogeninil1adominatedearlyphasesystemicinflammation AT tianyan potentantiinflammatoryresponsesroleofhydrogeninil1adominatedearlyphasesystemicinflammation AT yinhongling potentantiinflammatoryresponsesroleofhydrogeninil1adominatedearlyphasesystemicinflammation AT fuxuelian potentantiinflammatoryresponsesroleofhydrogeninil1adominatedearlyphasesystemicinflammation AT mazuan potentantiinflammatoryresponsesroleofhydrogeninil1adominatedearlyphasesystemicinflammation AT zhoujianjun potentantiinflammatoryresponsesroleofhydrogeninil1adominatedearlyphasesystemicinflammation AT yumin potentantiinflammatoryresponsesroleofhydrogeninil1adominatedearlyphasesystemicinflammation AT niqingbin potentantiinflammatoryresponsesroleofhydrogeninil1adominatedearlyphasesystemicinflammation AT tangwenjie potentantiinflammatoryresponsesroleofhydrogeninil1adominatedearlyphasesystemicinflammation |