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Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation

Introduction: It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice. However, the early dynamic process of the inflammatory response caused by lipopolysaccharide (LPS) and the anti-inflammatory effect of hydrogen has not been definitively...

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Autores principales: Wei, Youzhen, Wang, Kun, Zhang, Yafang, Duan, Yi, Tian, Yan, Yin, Hongling, Fu, Xuelian, Ma, Zuan, Zhou, Jianjun, Yu, Min, Ni, Qingbin, Tang, Wenjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10063881/
https://www.ncbi.nlm.nih.gov/pubmed/37007020
http://dx.doi.org/10.3389/fphar.2023.1138762
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author Wei, Youzhen
Wang, Kun
Zhang, Yafang
Duan, Yi
Tian, Yan
Yin, Hongling
Fu, Xuelian
Ma, Zuan
Zhou, Jianjun
Yu, Min
Ni, Qingbin
Tang, Wenjie
author_facet Wei, Youzhen
Wang, Kun
Zhang, Yafang
Duan, Yi
Tian, Yan
Yin, Hongling
Fu, Xuelian
Ma, Zuan
Zhou, Jianjun
Yu, Min
Ni, Qingbin
Tang, Wenjie
author_sort Wei, Youzhen
collection PubMed
description Introduction: It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice. However, the early dynamic process of the inflammatory response caused by lipopolysaccharide (LPS) and the anti-inflammatory effect of hydrogen has not been definitively reported. Methods: Inflammation in male C57/BL6J mice or RAW264.7 cells was induced with LPS, for which hydrogen was immediately administered until samples were taken. Pathological changes in lung tissue were assessed using hematoxylin and eosin (HE) staining. Levels of inflammatory factors in serum were determined using liquid protein chip. The mRNA levels of chemotactic factors in lung tissues, leukocytes, and peritoneal macrophages were measured by qRT-PCR. The expression levels of IL-1α and HIF-1α were measured by immunocytochemistry. Results: Hydrogen alleviated LPS-induced inflammatory infiltration in the lung tissues of mice. Among the 23 inflammatory factors screened, LPS-induced upregulation of IL-1α etc. was significantly inhibited by hydrogen within 1 hour. The mRNA expression of MCP-1, MIP-1α, G-CSF, and RANTES was inhibited obviously by hydrogen at 0.5 and 1 h in mouse peritoneal macrophages. In addition, hydrogen significantly blocked LPS or H2O2-induced upregulation of HIF-1α, and IL-1α in 0.5 h in RAW264.7 cells. Discussion: The results suggested that hydrogen is potentially inhibitive against inflammation by inhibiting HIF-1α and IL-1α release at early occurrence. The target of the inhibitive LPS-induced-inflammatory action of hydrogen is chemokines in macrophages in the peritoneal cavity. This study provides direct experimental evidence for quickly controlling inflammation with the translational application of a hydrogen-assisted protocol.
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spelling pubmed-100638812023-04-01 Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation Wei, Youzhen Wang, Kun Zhang, Yafang Duan, Yi Tian, Yan Yin, Hongling Fu, Xuelian Ma, Zuan Zhou, Jianjun Yu, Min Ni, Qingbin Tang, Wenjie Front Pharmacol Pharmacology Introduction: It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice. However, the early dynamic process of the inflammatory response caused by lipopolysaccharide (LPS) and the anti-inflammatory effect of hydrogen has not been definitively reported. Methods: Inflammation in male C57/BL6J mice or RAW264.7 cells was induced with LPS, for which hydrogen was immediately administered until samples were taken. Pathological changes in lung tissue were assessed using hematoxylin and eosin (HE) staining. Levels of inflammatory factors in serum were determined using liquid protein chip. The mRNA levels of chemotactic factors in lung tissues, leukocytes, and peritoneal macrophages were measured by qRT-PCR. The expression levels of IL-1α and HIF-1α were measured by immunocytochemistry. Results: Hydrogen alleviated LPS-induced inflammatory infiltration in the lung tissues of mice. Among the 23 inflammatory factors screened, LPS-induced upregulation of IL-1α etc. was significantly inhibited by hydrogen within 1 hour. The mRNA expression of MCP-1, MIP-1α, G-CSF, and RANTES was inhibited obviously by hydrogen at 0.5 and 1 h in mouse peritoneal macrophages. In addition, hydrogen significantly blocked LPS or H2O2-induced upregulation of HIF-1α, and IL-1α in 0.5 h in RAW264.7 cells. Discussion: The results suggested that hydrogen is potentially inhibitive against inflammation by inhibiting HIF-1α and IL-1α release at early occurrence. The target of the inhibitive LPS-induced-inflammatory action of hydrogen is chemokines in macrophages in the peritoneal cavity. This study provides direct experimental evidence for quickly controlling inflammation with the translational application of a hydrogen-assisted protocol. Frontiers Media S.A. 2023-03-17 /pmc/articles/PMC10063881/ /pubmed/37007020 http://dx.doi.org/10.3389/fphar.2023.1138762 Text en Copyright © 2023 Wei, Wang, Zhang, Duan, Tian, Yin, Fu, Ma, Zhou, Yu, Ni and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Wei, Youzhen
Wang, Kun
Zhang, Yafang
Duan, Yi
Tian, Yan
Yin, Hongling
Fu, Xuelian
Ma, Zuan
Zhou, Jianjun
Yu, Min
Ni, Qingbin
Tang, Wenjie
Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation
title Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation
title_full Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation
title_fullStr Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation
title_full_unstemmed Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation
title_short Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation
title_sort potent anti-inflammatory responses: role of hydrogen in il-1α dominated early phase systemic inflammation
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10063881/
https://www.ncbi.nlm.nih.gov/pubmed/37007020
http://dx.doi.org/10.3389/fphar.2023.1138762
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