Cargando…
β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations
The β‐lactoglobulin (β‐LG) was previously characterized as a mild antioxidant modulating cell viability. However, its biological action regarding endometrial stromal cell cytophysiology and function has never been considered. In this study, we investigated the influence of β‐LG on the cellular statu...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10064025/ https://www.ncbi.nlm.nih.gov/pubmed/36860157 http://dx.doi.org/10.1111/jcmm.17694 |
_version_ | 1785017821372088320 |
---|---|
author | Data, Krzysztof Marcinkowska, Klaudia Buś, Klaudia Valihrach, Lukas Pawlak, Edyta Śmieszek, Agnieszka |
author_facet | Data, Krzysztof Marcinkowska, Klaudia Buś, Klaudia Valihrach, Lukas Pawlak, Edyta Śmieszek, Agnieszka |
author_sort | Data, Krzysztof |
collection | PubMed |
description | The β‐lactoglobulin (β‐LG) was previously characterized as a mild antioxidant modulating cell viability. However, its biological action regarding endometrial stromal cell cytophysiology and function has never been considered. In this study, we investigated the influence of β‐LG on the cellular status of equine endometrial progenitor cells under oxidative stress. The study showed that β‐LG decreased the intracellular accumulation of reactive oxygen species, simultaneously ameliorating cell viability and exerting an anti‐apoptotic effect. However, at the transcriptional level, the reduced mRNA expression of pro‐apoptotic factors (i.e. BAX and BAD) was accompanied by decreased expression of mRNA for anti‐apoptotic BCL‐2 and genes coding antioxidant enzymes (CAT, SOD‐1, GPx). Still, we have also noted the positive effect of β‐LG on the expression profile of transcripts involved in endometrial viability and receptivity, including ITGB1, ENPP3, TUNAR and miR‐19b‐3p. Finally, the expression of master factors of endometrial decidualization, namely prolactin and IGFBP1, was increased in response to β‐LG, while non‐coding RNAs (ncRNAs), that is lncRNA MALAT1 and miR‐200b‐3p, were upregulated. Our findings indicate a novel potential role of β‐LG as a molecule regulating endometrial tissue functionality, promoting viability and normalizing the oxidative status of endometrial progenitor cells. The possible mechanism of β‐LG action includes the activation of ncRNAs essential for tissue regeneration, such as lncRNA MALAT‐1/TUNAR and miR‐19b‐3p/miR‐200b‐3p. |
format | Online Article Text |
id | pubmed-10064025 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100640252023-04-01 β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations Data, Krzysztof Marcinkowska, Klaudia Buś, Klaudia Valihrach, Lukas Pawlak, Edyta Śmieszek, Agnieszka J Cell Mol Med Original Articles The β‐lactoglobulin (β‐LG) was previously characterized as a mild antioxidant modulating cell viability. However, its biological action regarding endometrial stromal cell cytophysiology and function has never been considered. In this study, we investigated the influence of β‐LG on the cellular status of equine endometrial progenitor cells under oxidative stress. The study showed that β‐LG decreased the intracellular accumulation of reactive oxygen species, simultaneously ameliorating cell viability and exerting an anti‐apoptotic effect. However, at the transcriptional level, the reduced mRNA expression of pro‐apoptotic factors (i.e. BAX and BAD) was accompanied by decreased expression of mRNA for anti‐apoptotic BCL‐2 and genes coding antioxidant enzymes (CAT, SOD‐1, GPx). Still, we have also noted the positive effect of β‐LG on the expression profile of transcripts involved in endometrial viability and receptivity, including ITGB1, ENPP3, TUNAR and miR‐19b‐3p. Finally, the expression of master factors of endometrial decidualization, namely prolactin and IGFBP1, was increased in response to β‐LG, while non‐coding RNAs (ncRNAs), that is lncRNA MALAT1 and miR‐200b‐3p, were upregulated. Our findings indicate a novel potential role of β‐LG as a molecule regulating endometrial tissue functionality, promoting viability and normalizing the oxidative status of endometrial progenitor cells. The possible mechanism of β‐LG action includes the activation of ncRNAs essential for tissue regeneration, such as lncRNA MALAT‐1/TUNAR and miR‐19b‐3p/miR‐200b‐3p. John Wiley and Sons Inc. 2023-03-01 /pmc/articles/PMC10064025/ /pubmed/36860157 http://dx.doi.org/10.1111/jcmm.17694 Text en © 2023 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Data, Krzysztof Marcinkowska, Klaudia Buś, Klaudia Valihrach, Lukas Pawlak, Edyta Śmieszek, Agnieszka β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations |
title | β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations |
title_full | β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations |
title_fullStr | β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations |
title_full_unstemmed | β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations |
title_short | β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations |
title_sort | β‐lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncrna‐mrna‐mirna regulatory associations |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10064025/ https://www.ncbi.nlm.nih.gov/pubmed/36860157 http://dx.doi.org/10.1111/jcmm.17694 |
work_keys_str_mv | AT datakrzysztof blactoglobulinaffectstheoxidativestatusandviabilityofequineendometrialprogenitorcellsvialncrnamrnamirnaregulatoryassociations AT marcinkowskaklaudia blactoglobulinaffectstheoxidativestatusandviabilityofequineendometrialprogenitorcellsvialncrnamrnamirnaregulatoryassociations AT busklaudia blactoglobulinaffectstheoxidativestatusandviabilityofequineendometrialprogenitorcellsvialncrnamrnamirnaregulatoryassociations AT valihrachlukas blactoglobulinaffectstheoxidativestatusandviabilityofequineendometrialprogenitorcellsvialncrnamrnamirnaregulatoryassociations AT pawlakedyta blactoglobulinaffectstheoxidativestatusandviabilityofequineendometrialprogenitorcellsvialncrnamrnamirnaregulatoryassociations AT smieszekagnieszka blactoglobulinaffectstheoxidativestatusandviabilityofequineendometrialprogenitorcellsvialncrnamrnamirnaregulatoryassociations |