Cargando…

Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β‐catenin signalling

Tax1 binding protein 3 (Tax1bp3) is a PDZ domain‐containing protein that is overexpressed in cancer. Previous studies recognized Tax1bp3 as an inhibitor of β‐catenin. Till now it is not known whether Tax1bp3 regulates osteogenic and adipogenic differentiation of mesenchymal progenitor cells. In the...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Yi, Gan, Ying, Dong, Yuan, Zhou, Jie, Zhu, Endong, Yuan, Hairui, Li, Xiaoxia, Wang, Baoli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10064035/
https://www.ncbi.nlm.nih.gov/pubmed/36892460
http://dx.doi.org/10.1111/jcmm.17702
_version_ 1785017823811076096
author Wang, Yi
Gan, Ying
Dong, Yuan
Zhou, Jie
Zhu, Endong
Yuan, Hairui
Li, Xiaoxia
Wang, Baoli
author_facet Wang, Yi
Gan, Ying
Dong, Yuan
Zhou, Jie
Zhu, Endong
Yuan, Hairui
Li, Xiaoxia
Wang, Baoli
author_sort Wang, Yi
collection PubMed
description Tax1 binding protein 3 (Tax1bp3) is a PDZ domain‐containing protein that is overexpressed in cancer. Previous studies recognized Tax1bp3 as an inhibitor of β‐catenin. Till now it is not known whether Tax1bp3 regulates osteogenic and adipogenic differentiation of mesenchymal progenitor cells. In the current study, the data showed that Tax1bp3 was expressed in bone and was increased in the progenitor cells when induced toward osteoblast and adipocyte differentiation. The overexpression of Tax1bp3 in the progenitor cells inhibited osteogenic differentiation and conversely stimulated adipogenic differentiation, and the knockdown of Tax1bp3 affected the differentiation of the progenitor cells oppositely. Ex vivo experiments using the primary calvarial osteoblasts from osteoblast‐specific Tax1bp3 knock‐in mice also demonstrated the anti‐osteogenic and pro‐adipogenic function of Tax1bp3. Mechanistic investigations revealed that Tax1bp3 inhibited the activation of canonical Wnt/β‐catenin and bone morphogenetic proteins (BMPs)/Smads signalling pathways. Taken together, the current study has provided evidences demonstrating that Tax1bp3 inactivates Wnt/β‐catenin and BMPs/Smads signalling pathways and reciprocally regulates osteogenic and adipogenic differentiation from mesenchymal progenitor cells. The inactivation of Wnt/β‐catenin signalling may be involved in the reciprocal role of Tax1bp3.
format Online
Article
Text
id pubmed-10064035
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-100640352023-04-01 Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β‐catenin signalling Wang, Yi Gan, Ying Dong, Yuan Zhou, Jie Zhu, Endong Yuan, Hairui Li, Xiaoxia Wang, Baoli J Cell Mol Med Original Articles Tax1 binding protein 3 (Tax1bp3) is a PDZ domain‐containing protein that is overexpressed in cancer. Previous studies recognized Tax1bp3 as an inhibitor of β‐catenin. Till now it is not known whether Tax1bp3 regulates osteogenic and adipogenic differentiation of mesenchymal progenitor cells. In the current study, the data showed that Tax1bp3 was expressed in bone and was increased in the progenitor cells when induced toward osteoblast and adipocyte differentiation. The overexpression of Tax1bp3 in the progenitor cells inhibited osteogenic differentiation and conversely stimulated adipogenic differentiation, and the knockdown of Tax1bp3 affected the differentiation of the progenitor cells oppositely. Ex vivo experiments using the primary calvarial osteoblasts from osteoblast‐specific Tax1bp3 knock‐in mice also demonstrated the anti‐osteogenic and pro‐adipogenic function of Tax1bp3. Mechanistic investigations revealed that Tax1bp3 inhibited the activation of canonical Wnt/β‐catenin and bone morphogenetic proteins (BMPs)/Smads signalling pathways. Taken together, the current study has provided evidences demonstrating that Tax1bp3 inactivates Wnt/β‐catenin and BMPs/Smads signalling pathways and reciprocally regulates osteogenic and adipogenic differentiation from mesenchymal progenitor cells. The inactivation of Wnt/β‐catenin signalling may be involved in the reciprocal role of Tax1bp3. John Wiley and Sons Inc. 2023-03-09 /pmc/articles/PMC10064035/ /pubmed/36892460 http://dx.doi.org/10.1111/jcmm.17702 Text en © 2023 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Wang, Yi
Gan, Ying
Dong, Yuan
Zhou, Jie
Zhu, Endong
Yuan, Hairui
Li, Xiaoxia
Wang, Baoli
Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β‐catenin signalling
title Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β‐catenin signalling
title_full Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β‐catenin signalling
title_fullStr Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β‐catenin signalling
title_full_unstemmed Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β‐catenin signalling
title_short Tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating Wnt/β‐catenin signalling
title_sort tax1 binding protein 3 regulates osteogenic and adipogenic differentiation through inactivating wnt/β‐catenin signalling
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10064035/
https://www.ncbi.nlm.nih.gov/pubmed/36892460
http://dx.doi.org/10.1111/jcmm.17702
work_keys_str_mv AT wangyi tax1bindingprotein3regulatesosteogenicandadipogenicdifferentiationthroughinactivatingwntbcateninsignalling
AT ganying tax1bindingprotein3regulatesosteogenicandadipogenicdifferentiationthroughinactivatingwntbcateninsignalling
AT dongyuan tax1bindingprotein3regulatesosteogenicandadipogenicdifferentiationthroughinactivatingwntbcateninsignalling
AT zhoujie tax1bindingprotein3regulatesosteogenicandadipogenicdifferentiationthroughinactivatingwntbcateninsignalling
AT zhuendong tax1bindingprotein3regulatesosteogenicandadipogenicdifferentiationthroughinactivatingwntbcateninsignalling
AT yuanhairui tax1bindingprotein3regulatesosteogenicandadipogenicdifferentiationthroughinactivatingwntbcateninsignalling
AT lixiaoxia tax1bindingprotein3regulatesosteogenicandadipogenicdifferentiationthroughinactivatingwntbcateninsignalling
AT wangbaoli tax1bindingprotein3regulatesosteogenicandadipogenicdifferentiationthroughinactivatingwntbcateninsignalling