DNAJB7 is dispensable for male fertility in mice

BACKGROUND: DNAJBs are highly conserved proteins that are involved in various biological processes. Although several DNAJBs are highly expressed in the testis, the function of DNAJB7 in spermatogenesis and male fertility remains unclear. METHODS: To identify the role of DNAJB7 in the male reproducti...

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Autores principales: Bai, Shun, Hu, Meihong, Yu, Lina, Chen, Linjun, Zhou, Jidong, Wu, Limin, Xu, Bo, Jiang, Xiaohua, Zhang, Xindong, Tong, Xianhong, Yue, Qiuling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10064739/
https://www.ncbi.nlm.nih.gov/pubmed/37004113
http://dx.doi.org/10.1186/s12958-023-01086-6
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author Bai, Shun
Hu, Meihong
Yu, Lina
Chen, Linjun
Zhou, Jidong
Wu, Limin
Xu, Bo
Jiang, Xiaohua
Zhang, Xindong
Tong, Xianhong
Yue, Qiuling
author_facet Bai, Shun
Hu, Meihong
Yu, Lina
Chen, Linjun
Zhou, Jidong
Wu, Limin
Xu, Bo
Jiang, Xiaohua
Zhang, Xindong
Tong, Xianhong
Yue, Qiuling
author_sort Bai, Shun
collection PubMed
description BACKGROUND: DNAJBs are highly conserved proteins that are involved in various biological processes. Although several DNAJBs are highly expressed in the testis, the function of DNAJB7 in spermatogenesis and male fertility remains unclear. METHODS: To identify the role of DNAJB7 in the male reproduction process, Dnajb7-deficient mice were generated by the CRISPR/Cas9-mediated genome editing system. Histological and immunofluorescence assays were performed to analyze the phenotype of the Dnajb7 mutants. RESULTS: DNAJB7 is specifically expressed in haploid germ cells. Dnajb7 knockout mice are fertile and do not have any detectable defects in Sertoli cells, spermatogonia, meiotic and postmeiotic cells, indicating that DNAJB7 is not essential for spermatogenesis. CONCLUSIONS: Our findings suggest that DNAJB7 is dispensable for male fertility in mice, which could prevent duplicative work by other groups. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12958-023-01086-6.
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spelling pubmed-100647392023-04-01 DNAJB7 is dispensable for male fertility in mice Bai, Shun Hu, Meihong Yu, Lina Chen, Linjun Zhou, Jidong Wu, Limin Xu, Bo Jiang, Xiaohua Zhang, Xindong Tong, Xianhong Yue, Qiuling Reprod Biol Endocrinol Research BACKGROUND: DNAJBs are highly conserved proteins that are involved in various biological processes. Although several DNAJBs are highly expressed in the testis, the function of DNAJB7 in spermatogenesis and male fertility remains unclear. METHODS: To identify the role of DNAJB7 in the male reproduction process, Dnajb7-deficient mice were generated by the CRISPR/Cas9-mediated genome editing system. Histological and immunofluorescence assays were performed to analyze the phenotype of the Dnajb7 mutants. RESULTS: DNAJB7 is specifically expressed in haploid germ cells. Dnajb7 knockout mice are fertile and do not have any detectable defects in Sertoli cells, spermatogonia, meiotic and postmeiotic cells, indicating that DNAJB7 is not essential for spermatogenesis. CONCLUSIONS: Our findings suggest that DNAJB7 is dispensable for male fertility in mice, which could prevent duplicative work by other groups. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12958-023-01086-6. BioMed Central 2023-03-31 /pmc/articles/PMC10064739/ /pubmed/37004113 http://dx.doi.org/10.1186/s12958-023-01086-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Bai, Shun
Hu, Meihong
Yu, Lina
Chen, Linjun
Zhou, Jidong
Wu, Limin
Xu, Bo
Jiang, Xiaohua
Zhang, Xindong
Tong, Xianhong
Yue, Qiuling
DNAJB7 is dispensable for male fertility in mice
title DNAJB7 is dispensable for male fertility in mice
title_full DNAJB7 is dispensable for male fertility in mice
title_fullStr DNAJB7 is dispensable for male fertility in mice
title_full_unstemmed DNAJB7 is dispensable for male fertility in mice
title_short DNAJB7 is dispensable for male fertility in mice
title_sort dnajb7 is dispensable for male fertility in mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10064739/
https://www.ncbi.nlm.nih.gov/pubmed/37004113
http://dx.doi.org/10.1186/s12958-023-01086-6
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