Cargando…

Creatinine recovery from bovine urine under the effect of different times and temperatures of storage

Creatinine is a urinary marker used widely in ruminant’s experimental trials. However, despite its great importance no data were found in the literature about the best way to store bovine urine samples. In the sheep urine, was observed an increase in the urinary concentration of creatinine when it w...

Descripción completa

Detalles Bibliográficos
Autores principales: da Silva Júnior, Jarbas Miguel, Rennó, Luciana Navajas, Detmann, Edenio, Valadares Filho, Sebastião de Campos, Rodrigues, João Paulo Pacheco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10065246/
https://www.ncbi.nlm.nih.gov/pubmed/37000784
http://dx.doi.org/10.1371/journal.pone.0282145
_version_ 1785018063565881344
author da Silva Júnior, Jarbas Miguel
Rennó, Luciana Navajas
Detmann, Edenio
Valadares Filho, Sebastião de Campos
Rodrigues, João Paulo Pacheco
author_facet da Silva Júnior, Jarbas Miguel
Rennó, Luciana Navajas
Detmann, Edenio
Valadares Filho, Sebastião de Campos
Rodrigues, João Paulo Pacheco
author_sort da Silva Júnior, Jarbas Miguel
collection PubMed
description Creatinine is a urinary marker used widely in ruminant’s experimental trials. However, despite its great importance no data were found in the literature about the best way to store bovine urine samples. In the sheep urine, was observed an increase in the urinary concentration of creatinine when it was stored acidified (pH 2.5 to 3.5) at a temperature of 28 to 39 °C for 150 days of storage. Nevertheless, urine should be stored acidified (pH below 3) to avoid purine derivative degradation, So, aimed to evaluate creatinine recovery in bovine urine as a function of storage time and temperature. A total of 25 animals’ urine (10 Nellore cattle and 15 Holstein cattle) were collected. The urine (40 mL) was diluted in 160 mL of distilled water and its pH was corrected to a value lower than 3 using sulfuric acid drops. A sample of the diluted urine was analyzed to obtain the creatinine concentration reference value on the collection day. The remaining urine was fractionated and preserved at room temperature, cooled (4 °C) or frozen (-20 °C and -40 °C). In the urine of five Holstein cattle was added creatine solutions (20, 40 and 60 mg/dL) to evaluate the creatine to creatinine conservation. These urine samples were analyzed on different days after collection (1, 3, 7, 15, 30 and 45 days). The urine without any added creatine was analyzed on Days 1, 3, 7, 10, 15, 30, 45, 60, 90, 120, and 150 of storage. The addition of creatine in the urine caused an increase in the creatinine concentration (P < 0.05) after 30 days of storage at room temperature and under refrigeration (4 °C). In frozen samples, there was no change in creatinine concentration (P > 0.05). However, creatinine recovery was constant (P > 0.05) until day 15 of storage, regardless of the temperature used, when creatine was not added. After 30 days of storage, an effect of time and/or temperature was observed on creatinine recovery (P < 0.05). Urine samples can be stored at any temperature for up to 15 days after collection to estimate the creatinine concentration. Samples that need storage times longer than 15 days should be frozen (at -20 °C and -40 °C) to avoid creatinine concentration variation.
format Online
Article
Text
id pubmed-10065246
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-100652462023-04-01 Creatinine recovery from bovine urine under the effect of different times and temperatures of storage da Silva Júnior, Jarbas Miguel Rennó, Luciana Navajas Detmann, Edenio Valadares Filho, Sebastião de Campos Rodrigues, João Paulo Pacheco PLoS One Research Article Creatinine is a urinary marker used widely in ruminant’s experimental trials. However, despite its great importance no data were found in the literature about the best way to store bovine urine samples. In the sheep urine, was observed an increase in the urinary concentration of creatinine when it was stored acidified (pH 2.5 to 3.5) at a temperature of 28 to 39 °C for 150 days of storage. Nevertheless, urine should be stored acidified (pH below 3) to avoid purine derivative degradation, So, aimed to evaluate creatinine recovery in bovine urine as a function of storage time and temperature. A total of 25 animals’ urine (10 Nellore cattle and 15 Holstein cattle) were collected. The urine (40 mL) was diluted in 160 mL of distilled water and its pH was corrected to a value lower than 3 using sulfuric acid drops. A sample of the diluted urine was analyzed to obtain the creatinine concentration reference value on the collection day. The remaining urine was fractionated and preserved at room temperature, cooled (4 °C) or frozen (-20 °C and -40 °C). In the urine of five Holstein cattle was added creatine solutions (20, 40 and 60 mg/dL) to evaluate the creatine to creatinine conservation. These urine samples were analyzed on different days after collection (1, 3, 7, 15, 30 and 45 days). The urine without any added creatine was analyzed on Days 1, 3, 7, 10, 15, 30, 45, 60, 90, 120, and 150 of storage. The addition of creatine in the urine caused an increase in the creatinine concentration (P < 0.05) after 30 days of storage at room temperature and under refrigeration (4 °C). In frozen samples, there was no change in creatinine concentration (P > 0.05). However, creatinine recovery was constant (P > 0.05) until day 15 of storage, regardless of the temperature used, when creatine was not added. After 30 days of storage, an effect of time and/or temperature was observed on creatinine recovery (P < 0.05). Urine samples can be stored at any temperature for up to 15 days after collection to estimate the creatinine concentration. Samples that need storage times longer than 15 days should be frozen (at -20 °C and -40 °C) to avoid creatinine concentration variation. Public Library of Science 2023-03-31 /pmc/articles/PMC10065246/ /pubmed/37000784 http://dx.doi.org/10.1371/journal.pone.0282145 Text en © 2023 Silva Júnior et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
da Silva Júnior, Jarbas Miguel
Rennó, Luciana Navajas
Detmann, Edenio
Valadares Filho, Sebastião de Campos
Rodrigues, João Paulo Pacheco
Creatinine recovery from bovine urine under the effect of different times and temperatures of storage
title Creatinine recovery from bovine urine under the effect of different times and temperatures of storage
title_full Creatinine recovery from bovine urine under the effect of different times and temperatures of storage
title_fullStr Creatinine recovery from bovine urine under the effect of different times and temperatures of storage
title_full_unstemmed Creatinine recovery from bovine urine under the effect of different times and temperatures of storage
title_short Creatinine recovery from bovine urine under the effect of different times and temperatures of storage
title_sort creatinine recovery from bovine urine under the effect of different times and temperatures of storage
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10065246/
https://www.ncbi.nlm.nih.gov/pubmed/37000784
http://dx.doi.org/10.1371/journal.pone.0282145
work_keys_str_mv AT dasilvajuniorjarbasmiguel creatininerecoveryfrombovineurineundertheeffectofdifferenttimesandtemperaturesofstorage
AT rennoluciananavajas creatininerecoveryfrombovineurineundertheeffectofdifferenttimesandtemperaturesofstorage
AT detmannedenio creatininerecoveryfrombovineurineundertheeffectofdifferenttimesandtemperaturesofstorage
AT valadaresfilhosebastiaodecampos creatininerecoveryfrombovineurineundertheeffectofdifferenttimesandtemperaturesofstorage
AT rodriguesjoaopaulopacheco creatininerecoveryfrombovineurineundertheeffectofdifferenttimesandtemperaturesofstorage