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Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy
Organ decellularization creates cell-free, collagen-based extracellular matrices that can be used as scaffolds for tissue engineering applications. This technique has recently gained much attention, yet adequate scaffold repopulation and implantation remain a challenge. Specifically, there still nee...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10066218/ https://www.ncbi.nlm.nih.gov/pubmed/37002341 http://dx.doi.org/10.1038/s41598-023-31747-w |
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author | Corridon, Peter R. |
author_facet | Corridon, Peter R. |
author_sort | Corridon, Peter R. |
collection | PubMed |
description | Organ decellularization creates cell-free, collagen-based extracellular matrices that can be used as scaffolds for tissue engineering applications. This technique has recently gained much attention, yet adequate scaffold repopulation and implantation remain a challenge. Specifically, there still needs to be a greater understanding of scaffold responses post-transplantation and ways we can improve scaffold durability to withstand the in vivo environment. Recent studies have outlined vascular events that limit organ decellularization/recellularization scaffold viability for long-term transplantation. However, these insights have relied on in vitro/in vivo approaches that need enhanced spatial and temporal resolutions to investigate such issues at the microvascular level. This study uses intravital microscopy to gain instant feedback on their structure, function, and deformation dynamics. Thus, the objective of this study was to capture the effects of in vivo blood flow on the decellularized glomerulus, peritubular capillaries, and tubules after autologous and allogeneic orthotopic transplantation into rats. Large molecular weight dextran molecules labeled the vasculature. They revealed substantial degrees of translocation from glomerular and peritubular capillary tracks to the decellularized tubular epithelium and lumen as early as 12 h after transplantation, providing real-time evidence of the increases in microvascular permeability. Macromolecular extravasation persisted for a week, during which the decellularized microarchitecture was significantly and comparably compromised and thrombosed in both autologous and allogeneic approaches. These results indicate that in vivo multiphoton microscopy is a powerful approach for studying scaffold viability and identifying ways to promote scaffold longevity and vasculogenesis in bioartificial organs. |
format | Online Article Text |
id | pubmed-10066218 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-100662182023-04-02 Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy Corridon, Peter R. Sci Rep Article Organ decellularization creates cell-free, collagen-based extracellular matrices that can be used as scaffolds for tissue engineering applications. This technique has recently gained much attention, yet adequate scaffold repopulation and implantation remain a challenge. Specifically, there still needs to be a greater understanding of scaffold responses post-transplantation and ways we can improve scaffold durability to withstand the in vivo environment. Recent studies have outlined vascular events that limit organ decellularization/recellularization scaffold viability for long-term transplantation. However, these insights have relied on in vitro/in vivo approaches that need enhanced spatial and temporal resolutions to investigate such issues at the microvascular level. This study uses intravital microscopy to gain instant feedback on their structure, function, and deformation dynamics. Thus, the objective of this study was to capture the effects of in vivo blood flow on the decellularized glomerulus, peritubular capillaries, and tubules after autologous and allogeneic orthotopic transplantation into rats. Large molecular weight dextran molecules labeled the vasculature. They revealed substantial degrees of translocation from glomerular and peritubular capillary tracks to the decellularized tubular epithelium and lumen as early as 12 h after transplantation, providing real-time evidence of the increases in microvascular permeability. Macromolecular extravasation persisted for a week, during which the decellularized microarchitecture was significantly and comparably compromised and thrombosed in both autologous and allogeneic approaches. These results indicate that in vivo multiphoton microscopy is a powerful approach for studying scaffold viability and identifying ways to promote scaffold longevity and vasculogenesis in bioartificial organs. Nature Publishing Group UK 2023-03-31 /pmc/articles/PMC10066218/ /pubmed/37002341 http://dx.doi.org/10.1038/s41598-023-31747-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Corridon, Peter R. Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy |
title | Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy |
title_full | Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy |
title_fullStr | Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy |
title_full_unstemmed | Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy |
title_short | Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy |
title_sort | capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10066218/ https://www.ncbi.nlm.nih.gov/pubmed/37002341 http://dx.doi.org/10.1038/s41598-023-31747-w |
work_keys_str_mv | AT corridonpeterr capturingeffectsofbloodflowonthetransplanteddecellularizednephronwithintravitalmicroscopy |