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Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)

Formosatettix qinlingensis (Zheng, 1982) is a tiny grasshopper endemic to Qinling in China. For further study of its transcriptomic features, we obtained RNA-Seq data by Illumina HiSeq X Ten sequencing platform. Firstly, transcriptomic analysis showed that transcriptome read numbers of two female an...

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Autores principales: Liu, Yuxin, Li, Xuejuan, Lin, Liliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10066883/
https://www.ncbi.nlm.nih.gov/pubmed/37016680
http://dx.doi.org/10.7717/peerj.15123
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author Liu, Yuxin
Li, Xuejuan
Lin, Liliang
author_facet Liu, Yuxin
Li, Xuejuan
Lin, Liliang
author_sort Liu, Yuxin
collection PubMed
description Formosatettix qinlingensis (Zheng, 1982) is a tiny grasshopper endemic to Qinling in China. For further study of its transcriptomic features, we obtained RNA-Seq data by Illumina HiSeq X Ten sequencing platform. Firstly, transcriptomic analysis showed that transcriptome read numbers of two female and one male samples were 25,043,314, 24,429,905, and 25,034,457, respectively. We assembled 65,977 unigenes, their average length was 1,072.09 bp, and the length of N50 was 2,031 bp. The average lengths of F. qinlingensis female and male unigenes were 911.30 bp, and 941.82 bp, and the N50 lengths were 1,745 bp and 1,735 bp, respectively. Eight databases were used to annotate the functions of unigenes, and 23,268 functional unigenes were obtained. Besides, we also studied the body color, immunity and insecticide resistance of F. qinlingensis. Thirty-nine pigment-related genes were annotated. Some immunity genes and signaling pathways were found, such as JAK-STAT and Toll-LIKE receptor signaling pathways. There are also some insecticide resistance genes and signal pathways, like nAChR, GST and DDT. Further, some of these genes were differentially expressed in female and male samples, including pigment, immunity and insecticide resistance. The transcriptomic study of F. qinlingensis will provide data reference for gene prediction and molecular expression study of other Tetrigidae species in the future. Differential genetic screening of males and females provides a basis for studying sex and immune balance in insects.
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spelling pubmed-100668832023-04-03 Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae) Liu, Yuxin Li, Xuejuan Lin, Liliang PeerJ Bioinformatics Formosatettix qinlingensis (Zheng, 1982) is a tiny grasshopper endemic to Qinling in China. For further study of its transcriptomic features, we obtained RNA-Seq data by Illumina HiSeq X Ten sequencing platform. Firstly, transcriptomic analysis showed that transcriptome read numbers of two female and one male samples were 25,043,314, 24,429,905, and 25,034,457, respectively. We assembled 65,977 unigenes, their average length was 1,072.09 bp, and the length of N50 was 2,031 bp. The average lengths of F. qinlingensis female and male unigenes were 911.30 bp, and 941.82 bp, and the N50 lengths were 1,745 bp and 1,735 bp, respectively. Eight databases were used to annotate the functions of unigenes, and 23,268 functional unigenes were obtained. Besides, we also studied the body color, immunity and insecticide resistance of F. qinlingensis. Thirty-nine pigment-related genes were annotated. Some immunity genes and signaling pathways were found, such as JAK-STAT and Toll-LIKE receptor signaling pathways. There are also some insecticide resistance genes and signal pathways, like nAChR, GST and DDT. Further, some of these genes were differentially expressed in female and male samples, including pigment, immunity and insecticide resistance. The transcriptomic study of F. qinlingensis will provide data reference for gene prediction and molecular expression study of other Tetrigidae species in the future. Differential genetic screening of males and females provides a basis for studying sex and immune balance in insects. PeerJ Inc. 2023-03-30 /pmc/articles/PMC10066883/ /pubmed/37016680 http://dx.doi.org/10.7717/peerj.15123 Text en ©2023 Liu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Bioinformatics
Liu, Yuxin
Li, Xuejuan
Lin, Liliang
Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)
title Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)
title_full Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)
title_fullStr Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)
title_full_unstemmed Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)
title_short Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)
title_sort transcriptome of the pygmy grasshopper formosatettix qinlingensis (orthoptera: tetrigidae)
topic Bioinformatics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10066883/
https://www.ncbi.nlm.nih.gov/pubmed/37016680
http://dx.doi.org/10.7717/peerj.15123
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