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Green synthesis of glyco-CuInS(2) QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging
Glyco-quantum dots (glyco-QDs) have attracted significant interest in bioimaging applications, notably in cancer imaging, because they effectively combine the glycocluster effect with the exceptional optical properties of QDs. The key challenge now lies in how to eliminate the high heavy metal toxic...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10067196/ https://www.ncbi.nlm.nih.gov/pubmed/37005641 http://dx.doi.org/10.1186/s12951-023-01859-6 |
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author | Guan, Xiaolin Zhang, Liyuan Lai, Shoujun Zhang, Jiaming Wei, Jingyu Wang, Kang Zhang, Wentao Li, Chenghao Tong, Jinhui Lei, Ziqiang |
author_facet | Guan, Xiaolin Zhang, Liyuan Lai, Shoujun Zhang, Jiaming Wei, Jingyu Wang, Kang Zhang, Wentao Li, Chenghao Tong, Jinhui Lei, Ziqiang |
author_sort | Guan, Xiaolin |
collection | PubMed |
description | Glyco-quantum dots (glyco-QDs) have attracted significant interest in bioimaging applications, notably in cancer imaging, because they effectively combine the glycocluster effect with the exceptional optical properties of QDs. The key challenge now lies in how to eliminate the high heavy metal toxicity originating from traditional toxic Cd-based QDs for in vivo bioimaging. Herein, we report an eco-friendly pathway to prepare nontoxic Cd-free glyco-QDs in water by the “direct” reaction of thiol-ending monosaccharides with metal salts precursors. The formation of glyco-CuInS(2) QDs could be explained by a nucleation-growth mechanism following the LaMer model. As-prepared four glyco-CuInS(2) QDs were water-soluble, monodispersed, spherical in shape and exhibited size range of 3.0–4.0 nm. They exhibited well-separated dual emission in the visible region (500–590 nm) and near-infrared range (~ 827 nm), which may be attributable to visible excitonic emission and near-infrared surface defect emission. Meanwhile, the cell imaging displayed the reversibly distinct dual-color (green and red) fluorescence in tumor cells (HeLa, A549, MKN-45) and excellent membrane-targeting properties of glyco-CuInS(2) QDs based on their good biorecognition ability. Importantly, these QDs succeed in penetrating uniformly into the interior (the necrotic zone) of 3D multicellular tumor spheroids (MCTS) due to their high negative charge (zeta potential values ranging from − 23.9 to − 30.1 mV), which overcame the problem of poor penetration depth of existing QDs in in vitro spheroid models. So, confocal analysis confirmed their excellent ability to penetrate and label tumors. Thus, the successful application in in vivo bioimaging of these glyco-QDs verified that this design strategy is an effective, low cost and simple procedure for developing green nanoparticles as cheap and promising fluorescent bioprobes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-023-01859-6. |
format | Online Article Text |
id | pubmed-10067196 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-100671962023-04-03 Green synthesis of glyco-CuInS(2) QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging Guan, Xiaolin Zhang, Liyuan Lai, Shoujun Zhang, Jiaming Wei, Jingyu Wang, Kang Zhang, Wentao Li, Chenghao Tong, Jinhui Lei, Ziqiang J Nanobiotechnology Research Glyco-quantum dots (glyco-QDs) have attracted significant interest in bioimaging applications, notably in cancer imaging, because they effectively combine the glycocluster effect with the exceptional optical properties of QDs. The key challenge now lies in how to eliminate the high heavy metal toxicity originating from traditional toxic Cd-based QDs for in vivo bioimaging. Herein, we report an eco-friendly pathway to prepare nontoxic Cd-free glyco-QDs in water by the “direct” reaction of thiol-ending monosaccharides with metal salts precursors. The formation of glyco-CuInS(2) QDs could be explained by a nucleation-growth mechanism following the LaMer model. As-prepared four glyco-CuInS(2) QDs were water-soluble, monodispersed, spherical in shape and exhibited size range of 3.0–4.0 nm. They exhibited well-separated dual emission in the visible region (500–590 nm) and near-infrared range (~ 827 nm), which may be attributable to visible excitonic emission and near-infrared surface defect emission. Meanwhile, the cell imaging displayed the reversibly distinct dual-color (green and red) fluorescence in tumor cells (HeLa, A549, MKN-45) and excellent membrane-targeting properties of glyco-CuInS(2) QDs based on their good biorecognition ability. Importantly, these QDs succeed in penetrating uniformly into the interior (the necrotic zone) of 3D multicellular tumor spheroids (MCTS) due to their high negative charge (zeta potential values ranging from − 23.9 to − 30.1 mV), which overcame the problem of poor penetration depth of existing QDs in in vitro spheroid models. So, confocal analysis confirmed their excellent ability to penetrate and label tumors. Thus, the successful application in in vivo bioimaging of these glyco-QDs verified that this design strategy is an effective, low cost and simple procedure for developing green nanoparticles as cheap and promising fluorescent bioprobes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-023-01859-6. BioMed Central 2023-04-01 /pmc/articles/PMC10067196/ /pubmed/37005641 http://dx.doi.org/10.1186/s12951-023-01859-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Guan, Xiaolin Zhang, Liyuan Lai, Shoujun Zhang, Jiaming Wei, Jingyu Wang, Kang Zhang, Wentao Li, Chenghao Tong, Jinhui Lei, Ziqiang Green synthesis of glyco-CuInS(2) QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging |
title | Green synthesis of glyco-CuInS(2) QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging |
title_full | Green synthesis of glyco-CuInS(2) QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging |
title_fullStr | Green synthesis of glyco-CuInS(2) QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging |
title_full_unstemmed | Green synthesis of glyco-CuInS(2) QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging |
title_short | Green synthesis of glyco-CuInS(2) QDs with visible/NIR dual emission for 3D multicellular tumor spheroid and in vivo imaging |
title_sort | green synthesis of glyco-cuins(2) qds with visible/nir dual emission for 3d multicellular tumor spheroid and in vivo imaging |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10067196/ https://www.ncbi.nlm.nih.gov/pubmed/37005641 http://dx.doi.org/10.1186/s12951-023-01859-6 |
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