Cargando…

Hippocampal RNA sequencing in mice selectively bred for high and low activity

High and Low Activity strains of mice were bidirectionally selected for differences in open‐field activity (DeFries et al., 1978, Behavior Genetics, 8: 3–13) and subsequently inbred to use as a genetic model for studying anxiety‐like behaviors (Booher et al., 2021, Genes, Brain and Behavior, 20: e12...

Descripción completa

Detalles Bibliográficos
Autores principales: Booher, Winona C., Vanderlinden, Lauren A., Hall, Lucy A., Thomas, Aimee L., Evans, Luke M., Saba, Laura M., Ehringer, Marissa A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10067415/
https://www.ncbi.nlm.nih.gov/pubmed/36514243
http://dx.doi.org/10.1111/gbb.12832
_version_ 1785018464055853056
author Booher, Winona C.
Vanderlinden, Lauren A.
Hall, Lucy A.
Thomas, Aimee L.
Evans, Luke M.
Saba, Laura M.
Ehringer, Marissa A.
author_facet Booher, Winona C.
Vanderlinden, Lauren A.
Hall, Lucy A.
Thomas, Aimee L.
Evans, Luke M.
Saba, Laura M.
Ehringer, Marissa A.
author_sort Booher, Winona C.
collection PubMed
description High and Low Activity strains of mice were bidirectionally selected for differences in open‐field activity (DeFries et al., 1978, Behavior Genetics, 8: 3–13) and subsequently inbred to use as a genetic model for studying anxiety‐like behaviors (Booher et al., 2021, Genes, Brain and Behavior, 20: e12730). Hippocampal RNA‐sequencing of the High and Low Activity mice identified 3901 differentially expressed protein‐coding genes, with both sex‐dependent and sex‐independent effects. Functional enrichment analysis (PANTHER) highlighted 15 gene ontology terms, which allowed us to create a narrow list of 264 top candidate genes. Of the top candidate genes, 46 encoded four Complexes (I, II, IV and V) and two electron carriers (cytochrome c and ubiquinone) of the mitochondrial oxidative phosphorylation process. The most striking results were in the female high anxiety, Low Activity mice, where 39/46 genes relating to oxidative phosphorylation were upregulated. In addition, comparison of our top candidate genes with two previously curated High and Low Activity gene lists highlight 24 overlapping genes, where Ndufa13, which encodes the supernumerary subunit A13 of complex I, was the only gene to be included in all three lists. Mitochondrial dysfunction has recently been implicated as both a cause and effect of anxiety‐related disorders and thus should be further explored as a possible novel pharmaceutical treatment for anxiety disorders.
format Online
Article
Text
id pubmed-10067415
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Blackwell Publishing Ltd
record_format MEDLINE/PubMed
spelling pubmed-100674152023-04-04 Hippocampal RNA sequencing in mice selectively bred for high and low activity Booher, Winona C. Vanderlinden, Lauren A. Hall, Lucy A. Thomas, Aimee L. Evans, Luke M. Saba, Laura M. Ehringer, Marissa A. Genes Brain Behav Original Articles High and Low Activity strains of mice were bidirectionally selected for differences in open‐field activity (DeFries et al., 1978, Behavior Genetics, 8: 3–13) and subsequently inbred to use as a genetic model for studying anxiety‐like behaviors (Booher et al., 2021, Genes, Brain and Behavior, 20: e12730). Hippocampal RNA‐sequencing of the High and Low Activity mice identified 3901 differentially expressed protein‐coding genes, with both sex‐dependent and sex‐independent effects. Functional enrichment analysis (PANTHER) highlighted 15 gene ontology terms, which allowed us to create a narrow list of 264 top candidate genes. Of the top candidate genes, 46 encoded four Complexes (I, II, IV and V) and two electron carriers (cytochrome c and ubiquinone) of the mitochondrial oxidative phosphorylation process. The most striking results were in the female high anxiety, Low Activity mice, where 39/46 genes relating to oxidative phosphorylation were upregulated. In addition, comparison of our top candidate genes with two previously curated High and Low Activity gene lists highlight 24 overlapping genes, where Ndufa13, which encodes the supernumerary subunit A13 of complex I, was the only gene to be included in all three lists. Mitochondrial dysfunction has recently been implicated as both a cause and effect of anxiety‐related disorders and thus should be further explored as a possible novel pharmaceutical treatment for anxiety disorders. Blackwell Publishing Ltd 2022-12-13 /pmc/articles/PMC10067415/ /pubmed/36514243 http://dx.doi.org/10.1111/gbb.12832 Text en © 2022 The Authors. Genes, Brain and Behavior published by International Behavioural and Neural Genetics Society and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Booher, Winona C.
Vanderlinden, Lauren A.
Hall, Lucy A.
Thomas, Aimee L.
Evans, Luke M.
Saba, Laura M.
Ehringer, Marissa A.
Hippocampal RNA sequencing in mice selectively bred for high and low activity
title Hippocampal RNA sequencing in mice selectively bred for high and low activity
title_full Hippocampal RNA sequencing in mice selectively bred for high and low activity
title_fullStr Hippocampal RNA sequencing in mice selectively bred for high and low activity
title_full_unstemmed Hippocampal RNA sequencing in mice selectively bred for high and low activity
title_short Hippocampal RNA sequencing in mice selectively bred for high and low activity
title_sort hippocampal rna sequencing in mice selectively bred for high and low activity
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10067415/
https://www.ncbi.nlm.nih.gov/pubmed/36514243
http://dx.doi.org/10.1111/gbb.12832
work_keys_str_mv AT booherwinonac hippocampalrnasequencinginmiceselectivelybredforhighandlowactivity
AT vanderlindenlaurena hippocampalrnasequencinginmiceselectivelybredforhighandlowactivity
AT halllucya hippocampalrnasequencinginmiceselectivelybredforhighandlowactivity
AT thomasaimeel hippocampalrnasequencinginmiceselectivelybredforhighandlowactivity
AT evanslukem hippocampalrnasequencinginmiceselectivelybredforhighandlowactivity
AT sabalauram hippocampalrnasequencinginmiceselectivelybredforhighandlowactivity
AT ehringermarissaa hippocampalrnasequencinginmiceselectivelybredforhighandlowactivity