Cargando…
Productive HIV-1 infection of tissue macrophages by fusion with infected CD4(+) T cells
Macrophages are essential for HIV-1 pathogenesis and represent major viral reservoirs. Therefore, it is critical to understand macrophage infection, especially in tissue macrophages, which are widely infected in vivo, but poorly permissive to cell-free infection. Although cell-to-cell transmission o...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10067447/ https://www.ncbi.nlm.nih.gov/pubmed/36988579 http://dx.doi.org/10.1083/jcb.202205103 |
_version_ | 1785018472547221504 |
---|---|
author | Mascarau, Rémi Woottum, Marie Fromont, Léa Gence, Rémi Cantaloube-Ferrieu, Vincent Vahlas, Zoï Lévêque, Kevin Bertrand, Florent Beunon, Thomas Métais, Arnaud El Costa, Hicham Jabrane-Ferrat, Nabila Gallois, Yohan Guibert, Nicolas Davignon, Jean-Luc Favre, Gilles Maridonneau-Parini, Isabelle Poincloux, Renaud Lagane, Bernard Bénichou, Serge Raynaud-Messina, Brigitte Vérollet, Christel |
author_facet | Mascarau, Rémi Woottum, Marie Fromont, Léa Gence, Rémi Cantaloube-Ferrieu, Vincent Vahlas, Zoï Lévêque, Kevin Bertrand, Florent Beunon, Thomas Métais, Arnaud El Costa, Hicham Jabrane-Ferrat, Nabila Gallois, Yohan Guibert, Nicolas Davignon, Jean-Luc Favre, Gilles Maridonneau-Parini, Isabelle Poincloux, Renaud Lagane, Bernard Bénichou, Serge Raynaud-Messina, Brigitte Vérollet, Christel |
author_sort | Mascarau, Rémi |
collection | PubMed |
description | Macrophages are essential for HIV-1 pathogenesis and represent major viral reservoirs. Therefore, it is critical to understand macrophage infection, especially in tissue macrophages, which are widely infected in vivo, but poorly permissive to cell-free infection. Although cell-to-cell transmission of HIV-1 is a determinant mode of macrophage infection in vivo, how HIV-1 transfers toward macrophages remains elusive. Here, we demonstrate that fusion of infected CD4(+) T lymphocytes with human macrophages leads to their efficient and productive infection. Importantly, several tissue macrophage populations undergo this heterotypic cell fusion, including synovial, placental, lung alveolar, and tonsil macrophages. We also find that this mode of infection is modulated by the macrophage polarization state. This fusion process engages a specific short-lived adhesion structure and is controlled by the CD81 tetraspanin, which activates RhoA/ROCK-dependent actomyosin contractility in macrophages. Our study provides important insights into the mechanisms underlying infection of tissue-resident macrophages, and establishment of persistent cellular reservoirs in patients. |
format | Online Article Text |
id | pubmed-10067447 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-100674472023-04-04 Productive HIV-1 infection of tissue macrophages by fusion with infected CD4(+) T cells Mascarau, Rémi Woottum, Marie Fromont, Léa Gence, Rémi Cantaloube-Ferrieu, Vincent Vahlas, Zoï Lévêque, Kevin Bertrand, Florent Beunon, Thomas Métais, Arnaud El Costa, Hicham Jabrane-Ferrat, Nabila Gallois, Yohan Guibert, Nicolas Davignon, Jean-Luc Favre, Gilles Maridonneau-Parini, Isabelle Poincloux, Renaud Lagane, Bernard Bénichou, Serge Raynaud-Messina, Brigitte Vérollet, Christel J Cell Biol Article Macrophages are essential for HIV-1 pathogenesis and represent major viral reservoirs. Therefore, it is critical to understand macrophage infection, especially in tissue macrophages, which are widely infected in vivo, but poorly permissive to cell-free infection. Although cell-to-cell transmission of HIV-1 is a determinant mode of macrophage infection in vivo, how HIV-1 transfers toward macrophages remains elusive. Here, we demonstrate that fusion of infected CD4(+) T lymphocytes with human macrophages leads to their efficient and productive infection. Importantly, several tissue macrophage populations undergo this heterotypic cell fusion, including synovial, placental, lung alveolar, and tonsil macrophages. We also find that this mode of infection is modulated by the macrophage polarization state. This fusion process engages a specific short-lived adhesion structure and is controlled by the CD81 tetraspanin, which activates RhoA/ROCK-dependent actomyosin contractility in macrophages. Our study provides important insights into the mechanisms underlying infection of tissue-resident macrophages, and establishment of persistent cellular reservoirs in patients. Rockefeller University Press 2023-03-29 /pmc/articles/PMC10067447/ /pubmed/36988579 http://dx.doi.org/10.1083/jcb.202205103 Text en © 2023 Mascarau et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mascarau, Rémi Woottum, Marie Fromont, Léa Gence, Rémi Cantaloube-Ferrieu, Vincent Vahlas, Zoï Lévêque, Kevin Bertrand, Florent Beunon, Thomas Métais, Arnaud El Costa, Hicham Jabrane-Ferrat, Nabila Gallois, Yohan Guibert, Nicolas Davignon, Jean-Luc Favre, Gilles Maridonneau-Parini, Isabelle Poincloux, Renaud Lagane, Bernard Bénichou, Serge Raynaud-Messina, Brigitte Vérollet, Christel Productive HIV-1 infection of tissue macrophages by fusion with infected CD4(+) T cells |
title | Productive HIV-1 infection of tissue macrophages by fusion with infected CD4(+) T cells |
title_full | Productive HIV-1 infection of tissue macrophages by fusion with infected CD4(+) T cells |
title_fullStr | Productive HIV-1 infection of tissue macrophages by fusion with infected CD4(+) T cells |
title_full_unstemmed | Productive HIV-1 infection of tissue macrophages by fusion with infected CD4(+) T cells |
title_short | Productive HIV-1 infection of tissue macrophages by fusion with infected CD4(+) T cells |
title_sort | productive hiv-1 infection of tissue macrophages by fusion with infected cd4(+) t cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10067447/ https://www.ncbi.nlm.nih.gov/pubmed/36988579 http://dx.doi.org/10.1083/jcb.202205103 |
work_keys_str_mv | AT mascarauremi productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT woottummarie productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT fromontlea productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT genceremi productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT cantaloubeferrieuvincent productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT vahlaszoi productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT levequekevin productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT bertrandflorent productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT beunonthomas productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT metaisarnaud productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT elcostahicham productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT jabraneferratnabila productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT galloisyohan productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT guibertnicolas productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT davignonjeanluc productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT favregilles productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT maridonneaupariniisabelle productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT poinclouxrenaud productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT laganebernard productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT benichouserge productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT raynaudmessinabrigitte productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells AT verolletchristel productivehiv1infectionoftissuemacrophagesbyfusionwithinfectedcd4tcells |