Stunning of neutrophils accounts for the anti-inflammatory effects of clodronate liposomes

Clodronate liposomes (Clo-Lip) have been widely used to deplete mononuclear phagocytes (MoPh) to study the function of these cells in vivo. Here, we revisited the effects of Clo-Lip together with genetic models of MoPh deficiency, revealing that Clo-Lip exert their anti-inflammatory effects independ...

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Detalles Bibliográficos
Autores principales: Culemann, Stephan, Knab, Katharina, Euler, Maximilien, Wegner, Anja, Garibagaoglu, Hilal, Ackermann, Jochen, Fischer, Kim, Kienhöfer, Deborah, Crainiciuc, Georgiana, Hahn, Jonas, Grüneboom, Anika, Nimmerjahn, Falk, Uderhardt, Stefan, Hidalgo, Andrés, Schett, Georg, Hoffmann, Markus H., Krönke, Gerhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2023
Materias:
Brief Definitive Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10067541/
https://www.ncbi.nlm.nih.gov/pubmed/36976180
http://dx.doi.org/10.1084/jem.20220525
Descripción
Sumario:Clodronate liposomes (Clo-Lip) have been widely used to deplete mononuclear phagocytes (MoPh) to study the function of these cells in vivo. Here, we revisited the effects of Clo-Lip together with genetic models of MoPh deficiency, revealing that Clo-Lip exert their anti-inflammatory effects independent of MoPh. Notably, not only MoPh but also polymorphonuclear neutrophils (PMN) ingested Clo-Lip in vivo, which resulted in their functional arrest. Adoptive transfer of PMN, but not of MoPh, reversed the anti-inflammatory effects of Clo-Lip treatment, indicating that stunning of PMN rather than depletion of MoPh accounts for the anti-inflammatory effects of Clo-Lip in vivo. Our data highlight the need for a critical revision of the current literature on the role of MoPh in inflammation.

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