Cargando…
Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut
The maintenance of intestinal barrier function is essential for preventing different pathologies, such as the leaky gut syndrome (LGS), which is characterized by the passage of harmful agents, like bacteria, toxins, and viruses, into the bloodstream. Intestinal barrier integrity is controlled by sev...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10067918/ https://www.ncbi.nlm.nih.gov/pubmed/37020718 http://dx.doi.org/10.3389/fmicb.2023.1157164 |
_version_ | 1785018577850466304 |
---|---|
author | Algieri, Francesca Tanaskovic, Nina Rincon, Cindy Cardenas Notario, Elisabetta Braga, Daniele Pesole, Graziano Rusconi, Roberto Penna, Giuseppe Rescigno, Maria |
author_facet | Algieri, Francesca Tanaskovic, Nina Rincon, Cindy Cardenas Notario, Elisabetta Braga, Daniele Pesole, Graziano Rusconi, Roberto Penna, Giuseppe Rescigno, Maria |
author_sort | Algieri, Francesca |
collection | PubMed |
description | The maintenance of intestinal barrier function is essential for preventing different pathologies, such as the leaky gut syndrome (LGS), which is characterized by the passage of harmful agents, like bacteria, toxins, and viruses, into the bloodstream. Intestinal barrier integrity is controlled by several players, including the gut microbiota. Various molecules, called postbiotics, are released during the natural metabolic activity of the microbiota. Postbiotics can regulate host–microbe interactions, epithelial homeostasis, and have overall benefits for our health. In this work, we used in vitro and in vivo systems to demonstrate the role of Lactobacillus paracasei CNCM I-5220-derived postbiotic (LP-PBF) in preserving intestinal barrier integrity. We demonstrated in vitro that LP-PBF restored the morphology of tight junctions (TJs) that were altered upon Salmonella typhimurium exposure. In vivo, LP-PBF protected the gut vascular barrier and blocked S. typhimurium dissemination into the bloodstream. Interestingly, we found that LP-PBF interacts not only with the host cells, but also directly with S. typhimurium blocking its biofilm formation, partially due to the presence of biosurfactants. This study highlights that LP-PBF is beneficial in maintaining gut homeostasis due to the synergistic effect of its different components. These results suggest that LP-PBF could be utilized in managing several pathologies displaying an impaired intestinal barrier function. |
format | Online Article Text |
id | pubmed-10067918 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100679182023-04-04 Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut Algieri, Francesca Tanaskovic, Nina Rincon, Cindy Cardenas Notario, Elisabetta Braga, Daniele Pesole, Graziano Rusconi, Roberto Penna, Giuseppe Rescigno, Maria Front Microbiol Microbiology The maintenance of intestinal barrier function is essential for preventing different pathologies, such as the leaky gut syndrome (LGS), which is characterized by the passage of harmful agents, like bacteria, toxins, and viruses, into the bloodstream. Intestinal barrier integrity is controlled by several players, including the gut microbiota. Various molecules, called postbiotics, are released during the natural metabolic activity of the microbiota. Postbiotics can regulate host–microbe interactions, epithelial homeostasis, and have overall benefits for our health. In this work, we used in vitro and in vivo systems to demonstrate the role of Lactobacillus paracasei CNCM I-5220-derived postbiotic (LP-PBF) in preserving intestinal barrier integrity. We demonstrated in vitro that LP-PBF restored the morphology of tight junctions (TJs) that were altered upon Salmonella typhimurium exposure. In vivo, LP-PBF protected the gut vascular barrier and blocked S. typhimurium dissemination into the bloodstream. Interestingly, we found that LP-PBF interacts not only with the host cells, but also directly with S. typhimurium blocking its biofilm formation, partially due to the presence of biosurfactants. This study highlights that LP-PBF is beneficial in maintaining gut homeostasis due to the synergistic effect of its different components. These results suggest that LP-PBF could be utilized in managing several pathologies displaying an impaired intestinal barrier function. Frontiers Media S.A. 2023-03-20 /pmc/articles/PMC10067918/ /pubmed/37020718 http://dx.doi.org/10.3389/fmicb.2023.1157164 Text en Copyright © 2023 Algieri, Tanaskovic, Rincon, Notario, Braga, Pesole, Rusconi, Penna and Rescigno. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Algieri, Francesca Tanaskovic, Nina Rincon, Cindy Cardenas Notario, Elisabetta Braga, Daniele Pesole, Graziano Rusconi, Roberto Penna, Giuseppe Rescigno, Maria Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut |
title | Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut |
title_full | Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut |
title_fullStr | Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut |
title_full_unstemmed | Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut |
title_short | Lactobacillus paracasei CNCM I-5220-derived postbiotic protects from the leaky-gut |
title_sort | lactobacillus paracasei cncm i-5220-derived postbiotic protects from the leaky-gut |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10067918/ https://www.ncbi.nlm.nih.gov/pubmed/37020718 http://dx.doi.org/10.3389/fmicb.2023.1157164 |
work_keys_str_mv | AT algierifrancesca lactobacillusparacaseicncmi5220derivedpostbioticprotectsfromtheleakygut AT tanaskovicnina lactobacillusparacaseicncmi5220derivedpostbioticprotectsfromtheleakygut AT rinconcindycardenas lactobacillusparacaseicncmi5220derivedpostbioticprotectsfromtheleakygut AT notarioelisabetta lactobacillusparacaseicncmi5220derivedpostbioticprotectsfromtheleakygut AT bragadaniele lactobacillusparacaseicncmi5220derivedpostbioticprotectsfromtheleakygut AT pesolegraziano lactobacillusparacaseicncmi5220derivedpostbioticprotectsfromtheleakygut AT rusconiroberto lactobacillusparacaseicncmi5220derivedpostbioticprotectsfromtheleakygut AT pennagiuseppe lactobacillusparacaseicncmi5220derivedpostbioticprotectsfromtheleakygut AT rescignomaria lactobacillusparacaseicncmi5220derivedpostbioticprotectsfromtheleakygut |