Cargando…
Molecular identification of the chitinase genes in Aedes albopictus and essential roles of AaCht10 in pupal-adult transition
BACKGROUND: Aedes albopictus is an increasingly serious threat in public health due to it is vector of multiple arboviruses that cause devastating human diseases, as well as its widening distribution in recent years. Insecticide resistance is a serious problem worldwide that limits the efficacy of c...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10068161/ https://www.ncbi.nlm.nih.gov/pubmed/37005671 http://dx.doi.org/10.1186/s13071-023-05733-0 |
_version_ | 1785018623782289408 |
---|---|
author | An, Sha Liu, Wenjuan Fu, Jingwen Zhang, Zhong Zhang, Ruiling |
author_facet | An, Sha Liu, Wenjuan Fu, Jingwen Zhang, Zhong Zhang, Ruiling |
author_sort | An, Sha |
collection | PubMed |
description | BACKGROUND: Aedes albopictus is an increasingly serious threat in public health due to it is vector of multiple arboviruses that cause devastating human diseases, as well as its widening distribution in recent years. Insecticide resistance is a serious problem worldwide that limits the efficacy of chemical control strategies against Ae. albopictus. Chitinase genes have been widely recognized as attractive targets for the development of effective and environmentally safe insect management measures. METHODS: Chitinase genes of Ae. albopictus were identified and characterized on the basis of bioinformatics search of the referenced genome. Gene characterizations and phylogenetic relationships of chitinase genes were investigated, and spatio-temporal expression pattern of each chitinase gene was evaluated using qRT-PCR. RNA interference (RNAi) was used to suppress the expression of AaCht10, and the roles of AaCht10 were verified based on phynotype observations, chitin content analysis and hematoxylin and eosin (H&E) stain of epidermis and midgut. RESULTS: Altogether, 14 chitinase-related genes (12 chitinase genes and 2 IDGFs) encoding 17 proteins were identified. Phylogenetic analysis showed that all these AaChts were classified into seven groups, and most of them were gathered into group IX. Only AaCht5-1, AaCht10 and AaCht18 contained both catalytic and chitin-binding domains. Different AaChts displayed development- and tissue-specific expression profiling. Suppression of the expression of AaCht10 resulted in abnormal molting, increased mortality, decreased chitin content and thinning epicuticle, procuticle and midgut wall of pupa. CONCLUSIONS: Findings of the present study will aid in determining the biological functions of AaChts and also contribute to using AaChts as potential target for mosquito management. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05733-0. |
format | Online Article Text |
id | pubmed-10068161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-100681612023-04-04 Molecular identification of the chitinase genes in Aedes albopictus and essential roles of AaCht10 in pupal-adult transition An, Sha Liu, Wenjuan Fu, Jingwen Zhang, Zhong Zhang, Ruiling Parasit Vectors Research BACKGROUND: Aedes albopictus is an increasingly serious threat in public health due to it is vector of multiple arboviruses that cause devastating human diseases, as well as its widening distribution in recent years. Insecticide resistance is a serious problem worldwide that limits the efficacy of chemical control strategies against Ae. albopictus. Chitinase genes have been widely recognized as attractive targets for the development of effective and environmentally safe insect management measures. METHODS: Chitinase genes of Ae. albopictus were identified and characterized on the basis of bioinformatics search of the referenced genome. Gene characterizations and phylogenetic relationships of chitinase genes were investigated, and spatio-temporal expression pattern of each chitinase gene was evaluated using qRT-PCR. RNA interference (RNAi) was used to suppress the expression of AaCht10, and the roles of AaCht10 were verified based on phynotype observations, chitin content analysis and hematoxylin and eosin (H&E) stain of epidermis and midgut. RESULTS: Altogether, 14 chitinase-related genes (12 chitinase genes and 2 IDGFs) encoding 17 proteins were identified. Phylogenetic analysis showed that all these AaChts were classified into seven groups, and most of them were gathered into group IX. Only AaCht5-1, AaCht10 and AaCht18 contained both catalytic and chitin-binding domains. Different AaChts displayed development- and tissue-specific expression profiling. Suppression of the expression of AaCht10 resulted in abnormal molting, increased mortality, decreased chitin content and thinning epicuticle, procuticle and midgut wall of pupa. CONCLUSIONS: Findings of the present study will aid in determining the biological functions of AaChts and also contribute to using AaChts as potential target for mosquito management. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-023-05733-0. BioMed Central 2023-04-01 /pmc/articles/PMC10068161/ /pubmed/37005671 http://dx.doi.org/10.1186/s13071-023-05733-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research An, Sha Liu, Wenjuan Fu, Jingwen Zhang, Zhong Zhang, Ruiling Molecular identification of the chitinase genes in Aedes albopictus and essential roles of AaCht10 in pupal-adult transition |
title | Molecular identification of the chitinase genes in Aedes albopictus and essential roles of AaCht10 in pupal-adult transition |
title_full | Molecular identification of the chitinase genes in Aedes albopictus and essential roles of AaCht10 in pupal-adult transition |
title_fullStr | Molecular identification of the chitinase genes in Aedes albopictus and essential roles of AaCht10 in pupal-adult transition |
title_full_unstemmed | Molecular identification of the chitinase genes in Aedes albopictus and essential roles of AaCht10 in pupal-adult transition |
title_short | Molecular identification of the chitinase genes in Aedes albopictus and essential roles of AaCht10 in pupal-adult transition |
title_sort | molecular identification of the chitinase genes in aedes albopictus and essential roles of aacht10 in pupal-adult transition |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10068161/ https://www.ncbi.nlm.nih.gov/pubmed/37005671 http://dx.doi.org/10.1186/s13071-023-05733-0 |
work_keys_str_mv | AT ansha molecularidentificationofthechitinasegenesinaedesalbopictusandessentialrolesofaacht10inpupaladulttransition AT liuwenjuan molecularidentificationofthechitinasegenesinaedesalbopictusandessentialrolesofaacht10inpupaladulttransition AT fujingwen molecularidentificationofthechitinasegenesinaedesalbopictusandessentialrolesofaacht10inpupaladulttransition AT zhangzhong molecularidentificationofthechitinasegenesinaedesalbopictusandessentialrolesofaacht10inpupaladulttransition AT zhangruiling molecularidentificationofthechitinasegenesinaedesalbopictusandessentialrolesofaacht10inpupaladulttransition |