Cargando…
Adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin‐4 expression in the colonic epithelium
Adrenomedullin (AM) is a peptide with pleiotropic physiological functions that attenuates intestinal mucosal inflammation. However, the mechanism underpinning mucosal protection by AM is not fully understood, and its effect on intestinal epithelial cells remains unclear. Here, we investigated the ef...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10068314/ https://www.ncbi.nlm.nih.gov/pubmed/36799102 http://dx.doi.org/10.1002/2211-5463.13577 |
_version_ | 1785018650344816640 |
---|---|
author | Kawaguchi, Makiko Kataoka, Hiroaki Kiwaki, Takumi Weiting, Liang Nagata, Sayaka Kitamura, Kazuo Fukushima, Tsuyoshi |
author_facet | Kawaguchi, Makiko Kataoka, Hiroaki Kiwaki, Takumi Weiting, Liang Nagata, Sayaka Kitamura, Kazuo Fukushima, Tsuyoshi |
author_sort | Kawaguchi, Makiko |
collection | PubMed |
description | Adrenomedullin (AM) is a peptide with pleiotropic physiological functions that attenuates intestinal mucosal inflammation. However, the mechanism underpinning mucosal protection by AM is not fully understood, and its effect on intestinal epithelial cells remains unclear. Here, we investigated the effects of AM on junctional molecules in primary‐cultured murine intestinal epithelial cells and discovered that AM upregulates claudin‐4 expression. In a mouse model of dextran sulfate sodium‐induced colitis, AM administration also enhanced claudin‐4 expression and accelerated mucosal regeneration. Furthermore, AM reversed TNFα‐mediated downregulation of claudin‐4 and loss of cell–cell adhesion of the HCT116 human intestinal epithelial cell line in vitro. These results indicate that AM may enhance intestinal epithelial integrity by upregulating claudin‐4 expression. |
format | Online Article Text |
id | pubmed-10068314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100683142023-04-04 Adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin‐4 expression in the colonic epithelium Kawaguchi, Makiko Kataoka, Hiroaki Kiwaki, Takumi Weiting, Liang Nagata, Sayaka Kitamura, Kazuo Fukushima, Tsuyoshi FEBS Open Bio Research Articles Adrenomedullin (AM) is a peptide with pleiotropic physiological functions that attenuates intestinal mucosal inflammation. However, the mechanism underpinning mucosal protection by AM is not fully understood, and its effect on intestinal epithelial cells remains unclear. Here, we investigated the effects of AM on junctional molecules in primary‐cultured murine intestinal epithelial cells and discovered that AM upregulates claudin‐4 expression. In a mouse model of dextran sulfate sodium‐induced colitis, AM administration also enhanced claudin‐4 expression and accelerated mucosal regeneration. Furthermore, AM reversed TNFα‐mediated downregulation of claudin‐4 and loss of cell–cell adhesion of the HCT116 human intestinal epithelial cell line in vitro. These results indicate that AM may enhance intestinal epithelial integrity by upregulating claudin‐4 expression. John Wiley and Sons Inc. 2023-02-27 /pmc/articles/PMC10068314/ /pubmed/36799102 http://dx.doi.org/10.1002/2211-5463.13577 Text en © 2023 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Kawaguchi, Makiko Kataoka, Hiroaki Kiwaki, Takumi Weiting, Liang Nagata, Sayaka Kitamura, Kazuo Fukushima, Tsuyoshi Adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin‐4 expression in the colonic epithelium |
title | Adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin‐4 expression in the colonic epithelium |
title_full | Adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin‐4 expression in the colonic epithelium |
title_fullStr | Adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin‐4 expression in the colonic epithelium |
title_full_unstemmed | Adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin‐4 expression in the colonic epithelium |
title_short | Adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin‐4 expression in the colonic epithelium |
title_sort | adrenomedullin alleviates mucosal injury in experimental colitis and increases claudin‐4 expression in the colonic epithelium |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10068314/ https://www.ncbi.nlm.nih.gov/pubmed/36799102 http://dx.doi.org/10.1002/2211-5463.13577 |
work_keys_str_mv | AT kawaguchimakiko adrenomedullinalleviatesmucosalinjuryinexperimentalcolitisandincreasesclaudin4expressioninthecolonicepithelium AT kataokahiroaki adrenomedullinalleviatesmucosalinjuryinexperimentalcolitisandincreasesclaudin4expressioninthecolonicepithelium AT kiwakitakumi adrenomedullinalleviatesmucosalinjuryinexperimentalcolitisandincreasesclaudin4expressioninthecolonicepithelium AT weitingliang adrenomedullinalleviatesmucosalinjuryinexperimentalcolitisandincreasesclaudin4expressioninthecolonicepithelium AT nagatasayaka adrenomedullinalleviatesmucosalinjuryinexperimentalcolitisandincreasesclaudin4expressioninthecolonicepithelium AT kitamurakazuo adrenomedullinalleviatesmucosalinjuryinexperimentalcolitisandincreasesclaudin4expressioninthecolonicepithelium AT fukushimatsuyoshi adrenomedullinalleviatesmucosalinjuryinexperimentalcolitisandincreasesclaudin4expressioninthecolonicepithelium |