Cargando…

Two-point optical manipulation reveals mechanosensitive remodeling of cell–cell contacts in vivo

Biological tissues acquire reproducible shapes during development through dynamic cell behaviors. Most of these behaviors involve the remodeling of cell–cell contacts. During epithelial morphogenesis, contractile actomyosin networks remodel cell–cell contacts by shrinking and extending junctions bet...

Descripción completa

Detalles Bibliográficos
Autores principales: Nishizawa, Kenji, Lin, Shao-Zhen, Chardès, Claire, Rupprecht, Jean-François, Lenne, Pierre-François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10068846/
https://www.ncbi.nlm.nih.gov/pubmed/36947511
http://dx.doi.org/10.1073/pnas.2212389120
Descripción
Sumario:Biological tissues acquire reproducible shapes during development through dynamic cell behaviors. Most of these behaviors involve the remodeling of cell–cell contacts. During epithelial morphogenesis, contractile actomyosin networks remodel cell–cell contacts by shrinking and extending junctions between lateral cell surfaces. However, actomyosin networks not only generate mechanical stresses but also respond to them, confounding our understanding of how mechanical stresses remodel cell–cell contacts. Here, we develop a two-point optical manipulation method to impose different stress patterns on cell–cell contacts in the early epithelium of the Drosophila embryo. The technique allows us to produce junction extension and shrinkage through different push and pull manipulations at the edges of junctions. We use these observations to expand classical vertex-based models of tissue mechanics, incorporating negative and positive mechanosensitive feedback depending on the type of remodeling. In particular, we show that Myosin-II activity responds to junction strain rate and facilitates full junction shrinkage. Altogether our work provides insight into how stress produces efficient deformation of cell–cell contacts in vivo and identifies unanticipated mechanosensitive features of their remodeling.