Rapid determination of SARS-CoV-2 antibody neutralization titer using Bio-Rad Bio-Plex correlates strongly with pseudovirus-determined neutralization titer

Accurate and rapid evaluation of SARS-CoV-2 half-maximal neutralizing antibody (nAb) titer (NT50) is an important research tool for measuring nAb responses after prophylaxis or therapeutics for COVID-19 prevention and management. Compared with ACE2-competitive enzyme immunoassays for nAb detection,...

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Autores principales: Heaps, Amy L., Sobolewski, Michele D., Jacobs, Jana L., Gordon, Kelley C., Haidar, Ghady, Mellors, John W., Parikh, Urvi M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069795/
https://www.ncbi.nlm.nih.gov/pubmed/36996908
http://dx.doi.org/10.1016/j.jviromet.2023.114726
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author Heaps, Amy L.
Sobolewski, Michele D.
Jacobs, Jana L.
Gordon, Kelley C.
Haidar, Ghady
Mellors, John W.
Parikh, Urvi M.
author_facet Heaps, Amy L.
Sobolewski, Michele D.
Jacobs, Jana L.
Gordon, Kelley C.
Haidar, Ghady
Mellors, John W.
Parikh, Urvi M.
author_sort Heaps, Amy L.
collection PubMed
description Accurate and rapid evaluation of SARS-CoV-2 half-maximal neutralizing antibody (nAb) titer (NT50) is an important research tool for measuring nAb responses after prophylaxis or therapeutics for COVID-19 prevention and management. Compared with ACE2-competitive enzyme immunoassays for nAb detection, pseudovirus assays remain low-throughput and labor intensive. A novel application of the Bio-Rad Bio-Plex Pro Human SARS-CoV-2 D614G S1 Variant nAb Assay was used to determine NT50 from COVID-19-vaccinated individuals and showed strong correlation to a laboratory-developed SARS-CoV-2 pseudovirus nAb assay. The Bio-Plex nAb assay could provide a rapid, high-throughput, culture-free method for NT50 determination in sera.
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spelling pubmed-100697952023-04-04 Rapid determination of SARS-CoV-2 antibody neutralization titer using Bio-Rad Bio-Plex correlates strongly with pseudovirus-determined neutralization titer Heaps, Amy L. Sobolewski, Michele D. Jacobs, Jana L. Gordon, Kelley C. Haidar, Ghady Mellors, John W. Parikh, Urvi M. J Virol Methods Article Accurate and rapid evaluation of SARS-CoV-2 half-maximal neutralizing antibody (nAb) titer (NT50) is an important research tool for measuring nAb responses after prophylaxis or therapeutics for COVID-19 prevention and management. Compared with ACE2-competitive enzyme immunoassays for nAb detection, pseudovirus assays remain low-throughput and labor intensive. A novel application of the Bio-Rad Bio-Plex Pro Human SARS-CoV-2 D614G S1 Variant nAb Assay was used to determine NT50 from COVID-19-vaccinated individuals and showed strong correlation to a laboratory-developed SARS-CoV-2 pseudovirus nAb assay. The Bio-Plex nAb assay could provide a rapid, high-throughput, culture-free method for NT50 determination in sera. Published by Elsevier B.V. 2023-06 2023-03-28 /pmc/articles/PMC10069795/ /pubmed/36996908 http://dx.doi.org/10.1016/j.jviromet.2023.114726 Text en © 2023 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Heaps, Amy L.
Sobolewski, Michele D.
Jacobs, Jana L.
Gordon, Kelley C.
Haidar, Ghady
Mellors, John W.
Parikh, Urvi M.
Rapid determination of SARS-CoV-2 antibody neutralization titer using Bio-Rad Bio-Plex correlates strongly with pseudovirus-determined neutralization titer
title Rapid determination of SARS-CoV-2 antibody neutralization titer using Bio-Rad Bio-Plex correlates strongly with pseudovirus-determined neutralization titer
title_full Rapid determination of SARS-CoV-2 antibody neutralization titer using Bio-Rad Bio-Plex correlates strongly with pseudovirus-determined neutralization titer
title_fullStr Rapid determination of SARS-CoV-2 antibody neutralization titer using Bio-Rad Bio-Plex correlates strongly with pseudovirus-determined neutralization titer
title_full_unstemmed Rapid determination of SARS-CoV-2 antibody neutralization titer using Bio-Rad Bio-Plex correlates strongly with pseudovirus-determined neutralization titer
title_short Rapid determination of SARS-CoV-2 antibody neutralization titer using Bio-Rad Bio-Plex correlates strongly with pseudovirus-determined neutralization titer
title_sort rapid determination of sars-cov-2 antibody neutralization titer using bio-rad bio-plex correlates strongly with pseudovirus-determined neutralization titer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069795/
https://www.ncbi.nlm.nih.gov/pubmed/36996908
http://dx.doi.org/10.1016/j.jviromet.2023.114726
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