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Computational modeling of cambium activity provides a regulatory framework for simulating radial plant growth

Precise organization of growing structures is a fundamental process in developmental biology. In plants, radial growth is mediated by the cambium, a stem cell niche continuously producing wood (xylem) and bast (phloem) in a strictly bidirectional manner. While this process contributes large parts to...

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Autores principales: Lebovka, Ivan, Hay Mele, Bruno, Liu, Xiaomin, Zakieva, Alexandra, Schlamp, Theresa, Gursanscky, Nial Rau, Merks, Roeland MH, Großeholz, Ruth, Greb, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069871/
https://www.ncbi.nlm.nih.gov/pubmed/36897801
http://dx.doi.org/10.7554/eLife.66627
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author Lebovka, Ivan
Hay Mele, Bruno
Liu, Xiaomin
Zakieva, Alexandra
Schlamp, Theresa
Gursanscky, Nial Rau
Merks, Roeland MH
Großeholz, Ruth
Greb, Thomas
author_facet Lebovka, Ivan
Hay Mele, Bruno
Liu, Xiaomin
Zakieva, Alexandra
Schlamp, Theresa
Gursanscky, Nial Rau
Merks, Roeland MH
Großeholz, Ruth
Greb, Thomas
author_sort Lebovka, Ivan
collection PubMed
description Precise organization of growing structures is a fundamental process in developmental biology. In plants, radial growth is mediated by the cambium, a stem cell niche continuously producing wood (xylem) and bast (phloem) in a strictly bidirectional manner. While this process contributes large parts to terrestrial biomass, cambium dynamics eludes direct experimental access due to obstacles in live-cell imaging. Here, we present a cell-based computational model visualizing cambium activity and integrating the function of central cambium regulators. Performing iterative comparisons of plant and model anatomies, we conclude that the receptor-like kinase PXY and its ligand CLE41 are part of a minimal framework sufficient for instructing tissue organization. By integrating tissue-specific cell wall stiffness values, we moreover probe the influence of physical constraints on tissue geometry. Our model highlights the role of intercellular communication within the cambium and shows that a limited number of factors are sufficient to create radial growth by bidirectional tissue production.
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spelling pubmed-100698712023-04-04 Computational modeling of cambium activity provides a regulatory framework for simulating radial plant growth Lebovka, Ivan Hay Mele, Bruno Liu, Xiaomin Zakieva, Alexandra Schlamp, Theresa Gursanscky, Nial Rau Merks, Roeland MH Großeholz, Ruth Greb, Thomas eLife Plant Biology Precise organization of growing structures is a fundamental process in developmental biology. In plants, radial growth is mediated by the cambium, a stem cell niche continuously producing wood (xylem) and bast (phloem) in a strictly bidirectional manner. While this process contributes large parts to terrestrial biomass, cambium dynamics eludes direct experimental access due to obstacles in live-cell imaging. Here, we present a cell-based computational model visualizing cambium activity and integrating the function of central cambium regulators. Performing iterative comparisons of plant and model anatomies, we conclude that the receptor-like kinase PXY and its ligand CLE41 are part of a minimal framework sufficient for instructing tissue organization. By integrating tissue-specific cell wall stiffness values, we moreover probe the influence of physical constraints on tissue geometry. Our model highlights the role of intercellular communication within the cambium and shows that a limited number of factors are sufficient to create radial growth by bidirectional tissue production. eLife Sciences Publications, Ltd 2023-03-10 /pmc/articles/PMC10069871/ /pubmed/36897801 http://dx.doi.org/10.7554/eLife.66627 Text en © 2023, Lebovka et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Plant Biology
Lebovka, Ivan
Hay Mele, Bruno
Liu, Xiaomin
Zakieva, Alexandra
Schlamp, Theresa
Gursanscky, Nial Rau
Merks, Roeland MH
Großeholz, Ruth
Greb, Thomas
Computational modeling of cambium activity provides a regulatory framework for simulating radial plant growth
title Computational modeling of cambium activity provides a regulatory framework for simulating radial plant growth
title_full Computational modeling of cambium activity provides a regulatory framework for simulating radial plant growth
title_fullStr Computational modeling of cambium activity provides a regulatory framework for simulating radial plant growth
title_full_unstemmed Computational modeling of cambium activity provides a regulatory framework for simulating radial plant growth
title_short Computational modeling of cambium activity provides a regulatory framework for simulating radial plant growth
title_sort computational modeling of cambium activity provides a regulatory framework for simulating radial plant growth
topic Plant Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069871/
https://www.ncbi.nlm.nih.gov/pubmed/36897801
http://dx.doi.org/10.7554/eLife.66627
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