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Specific suppression of long terminal repeat retrotransposon mobilization in plants

The tissue culture passage necessary for the generation of transgenic plants induces genome instability. This instability predominantly involves the uncontrolled mobilization of LTR retrotransposons (LTR-TEs), which are the most abundant class of mobile genetic elements in plant genomes. Here, we de...

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Autores principales: Brestovitsky, Anna, Iwasaki, Mayumi, Cho, Jungnam, Adulyanukosol, Natthawut, Paszkowski, Jerzy, Catoni, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069891/
https://www.ncbi.nlm.nih.gov/pubmed/36583226
http://dx.doi.org/10.1093/plphys/kiac605
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author Brestovitsky, Anna
Iwasaki, Mayumi
Cho, Jungnam
Adulyanukosol, Natthawut
Paszkowski, Jerzy
Catoni, Marco
author_facet Brestovitsky, Anna
Iwasaki, Mayumi
Cho, Jungnam
Adulyanukosol, Natthawut
Paszkowski, Jerzy
Catoni, Marco
author_sort Brestovitsky, Anna
collection PubMed
description The tissue culture passage necessary for the generation of transgenic plants induces genome instability. This instability predominantly involves the uncontrolled mobilization of LTR retrotransposons (LTR-TEs), which are the most abundant class of mobile genetic elements in plant genomes. Here, we demonstrate that in conditions inductive for high LTR-TE mobilization, like abiotic stress in Arabidopsis (Arabidopsis thaliana) and callus culture in rice (Oryza sativa), application of the reverse transcriptase (RT) inhibitor known as Tenofovir substantially affects LTR-TE RT activity without interfering with plant development. We observed that Tenofovir reduces extrachromosomal DNA accumulation and prevents new genomic integrations of the active LTR-TE ONSEN in heat-stressed Arabidopsis seedlings, and transposons of O. sativa 17 and 19 (Tos17 and Tos19) in rice calli. In addition, Tenofovir allows the recovery of plants free from new LTR-TE insertions. We propose the use of Tenofovir as a tool for studies of LTR-TE transposition and for limiting genetic instabilities of plants derived from tissue culture.
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spelling pubmed-100698912023-04-04 Specific suppression of long terminal repeat retrotransposon mobilization in plants Brestovitsky, Anna Iwasaki, Mayumi Cho, Jungnam Adulyanukosol, Natthawut Paszkowski, Jerzy Catoni, Marco Plant Physiol Research Article The tissue culture passage necessary for the generation of transgenic plants induces genome instability. This instability predominantly involves the uncontrolled mobilization of LTR retrotransposons (LTR-TEs), which are the most abundant class of mobile genetic elements in plant genomes. Here, we demonstrate that in conditions inductive for high LTR-TE mobilization, like abiotic stress in Arabidopsis (Arabidopsis thaliana) and callus culture in rice (Oryza sativa), application of the reverse transcriptase (RT) inhibitor known as Tenofovir substantially affects LTR-TE RT activity without interfering with plant development. We observed that Tenofovir reduces extrachromosomal DNA accumulation and prevents new genomic integrations of the active LTR-TE ONSEN in heat-stressed Arabidopsis seedlings, and transposons of O. sativa 17 and 19 (Tos17 and Tos19) in rice calli. In addition, Tenofovir allows the recovery of plants free from new LTR-TE insertions. We propose the use of Tenofovir as a tool for studies of LTR-TE transposition and for limiting genetic instabilities of plants derived from tissue culture. Oxford University Press 2022-12-30 /pmc/articles/PMC10069891/ /pubmed/36583226 http://dx.doi.org/10.1093/plphys/kiac605 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Brestovitsky, Anna
Iwasaki, Mayumi
Cho, Jungnam
Adulyanukosol, Natthawut
Paszkowski, Jerzy
Catoni, Marco
Specific suppression of long terminal repeat retrotransposon mobilization in plants
title Specific suppression of long terminal repeat retrotransposon mobilization in plants
title_full Specific suppression of long terminal repeat retrotransposon mobilization in plants
title_fullStr Specific suppression of long terminal repeat retrotransposon mobilization in plants
title_full_unstemmed Specific suppression of long terminal repeat retrotransposon mobilization in plants
title_short Specific suppression of long terminal repeat retrotransposon mobilization in plants
title_sort specific suppression of long terminal repeat retrotransposon mobilization in plants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069891/
https://www.ncbi.nlm.nih.gov/pubmed/36583226
http://dx.doi.org/10.1093/plphys/kiac605
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