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Specific suppression of long terminal repeat retrotransposon mobilization in plants
The tissue culture passage necessary for the generation of transgenic plants induces genome instability. This instability predominantly involves the uncontrolled mobilization of LTR retrotransposons (LTR-TEs), which are the most abundant class of mobile genetic elements in plant genomes. Here, we de...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069891/ https://www.ncbi.nlm.nih.gov/pubmed/36583226 http://dx.doi.org/10.1093/plphys/kiac605 |
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author | Brestovitsky, Anna Iwasaki, Mayumi Cho, Jungnam Adulyanukosol, Natthawut Paszkowski, Jerzy Catoni, Marco |
author_facet | Brestovitsky, Anna Iwasaki, Mayumi Cho, Jungnam Adulyanukosol, Natthawut Paszkowski, Jerzy Catoni, Marco |
author_sort | Brestovitsky, Anna |
collection | PubMed |
description | The tissue culture passage necessary for the generation of transgenic plants induces genome instability. This instability predominantly involves the uncontrolled mobilization of LTR retrotransposons (LTR-TEs), which are the most abundant class of mobile genetic elements in plant genomes. Here, we demonstrate that in conditions inductive for high LTR-TE mobilization, like abiotic stress in Arabidopsis (Arabidopsis thaliana) and callus culture in rice (Oryza sativa), application of the reverse transcriptase (RT) inhibitor known as Tenofovir substantially affects LTR-TE RT activity without interfering with plant development. We observed that Tenofovir reduces extrachromosomal DNA accumulation and prevents new genomic integrations of the active LTR-TE ONSEN in heat-stressed Arabidopsis seedlings, and transposons of O. sativa 17 and 19 (Tos17 and Tos19) in rice calli. In addition, Tenofovir allows the recovery of plants free from new LTR-TE insertions. We propose the use of Tenofovir as a tool for studies of LTR-TE transposition and for limiting genetic instabilities of plants derived from tissue culture. |
format | Online Article Text |
id | pubmed-10069891 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-100698912023-04-04 Specific suppression of long terminal repeat retrotransposon mobilization in plants Brestovitsky, Anna Iwasaki, Mayumi Cho, Jungnam Adulyanukosol, Natthawut Paszkowski, Jerzy Catoni, Marco Plant Physiol Research Article The tissue culture passage necessary for the generation of transgenic plants induces genome instability. This instability predominantly involves the uncontrolled mobilization of LTR retrotransposons (LTR-TEs), which are the most abundant class of mobile genetic elements in plant genomes. Here, we demonstrate that in conditions inductive for high LTR-TE mobilization, like abiotic stress in Arabidopsis (Arabidopsis thaliana) and callus culture in rice (Oryza sativa), application of the reverse transcriptase (RT) inhibitor known as Tenofovir substantially affects LTR-TE RT activity without interfering with plant development. We observed that Tenofovir reduces extrachromosomal DNA accumulation and prevents new genomic integrations of the active LTR-TE ONSEN in heat-stressed Arabidopsis seedlings, and transposons of O. sativa 17 and 19 (Tos17 and Tos19) in rice calli. In addition, Tenofovir allows the recovery of plants free from new LTR-TE insertions. We propose the use of Tenofovir as a tool for studies of LTR-TE transposition and for limiting genetic instabilities of plants derived from tissue culture. Oxford University Press 2022-12-30 /pmc/articles/PMC10069891/ /pubmed/36583226 http://dx.doi.org/10.1093/plphys/kiac605 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Brestovitsky, Anna Iwasaki, Mayumi Cho, Jungnam Adulyanukosol, Natthawut Paszkowski, Jerzy Catoni, Marco Specific suppression of long terminal repeat retrotransposon mobilization in plants |
title | Specific suppression of long terminal repeat retrotransposon mobilization in plants |
title_full | Specific suppression of long terminal repeat retrotransposon mobilization in plants |
title_fullStr | Specific suppression of long terminal repeat retrotransposon mobilization in plants |
title_full_unstemmed | Specific suppression of long terminal repeat retrotransposon mobilization in plants |
title_short | Specific suppression of long terminal repeat retrotransposon mobilization in plants |
title_sort | specific suppression of long terminal repeat retrotransposon mobilization in plants |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069891/ https://www.ncbi.nlm.nih.gov/pubmed/36583226 http://dx.doi.org/10.1093/plphys/kiac605 |
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