Cargando…

Defining the lipidome of Arabidopsis leaf mitochondria: Specific lipid complement and biosynthesis capacity

Mitochondria are often considered as the power stations of the cell, playing critical roles in various biological processes such as cellular respiration, photosynthesis, stress responses, and programmed cell death. To maintain the structural and functional integrities of mitochondria, it is crucial...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yi-Tse, Senkler, Jennifer, Herrfurth, Cornelia, Braun, Hans-Peter, Feussner, Ivo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069894/
https://www.ncbi.nlm.nih.gov/pubmed/36691154
http://dx.doi.org/10.1093/plphys/kiad035
_version_ 1785018936510644224
author Liu, Yi-Tse
Senkler, Jennifer
Herrfurth, Cornelia
Braun, Hans-Peter
Feussner, Ivo
author_facet Liu, Yi-Tse
Senkler, Jennifer
Herrfurth, Cornelia
Braun, Hans-Peter
Feussner, Ivo
author_sort Liu, Yi-Tse
collection PubMed
description Mitochondria are often considered as the power stations of the cell, playing critical roles in various biological processes such as cellular respiration, photosynthesis, stress responses, and programmed cell death. To maintain the structural and functional integrities of mitochondria, it is crucial to achieve a defined membrane lipid composition between different lipid classes wherein specific proportions of individual lipid species are present. Although mitochondria are capable of self-synthesizing a few lipid classes, many phospholipids are synthesized in the endoplasmic reticulum and transferred to mitochondria via membrane contact sites, as mitochondria are excluded from the vesicular transportation pathway. However, knowledge on the capability of lipid biosynthesis in mitochondria and the precise mechanism of maintaining the homeostasis of mitochondrial lipids is still scarce. Here we describe the lipidome of mitochondria isolated from Arabidopsis (Arabidopsis thaliana) leaves, including the molecular species of glycerolipids, sphingolipids, and sterols, to depict the lipid landscape of mitochondrial membranes. In addition, we define proteins involved in lipid metabolism by proteomic analysis and compare our data with mitochondria from cell cultures since they still serve as model systems. Proteins putatively localized to the membrane contact sites are proposed based on the proteomic results and online databases. Collectively, our results suggest that leaf mitochondria are capable—with the assistance of membrane contact site-localized proteins—of generating several lipid classes including phosphatidylethanolamines, cardiolipins, diacylgalactosylglycerols, and free sterols. We anticipate our work to be a foundation to further investigate the functional roles of lipids and their involvement in biochemical reactions in plant mitochondria.
format Online
Article
Text
id pubmed-10069894
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-100698942023-04-04 Defining the lipidome of Arabidopsis leaf mitochondria: Specific lipid complement and biosynthesis capacity Liu, Yi-Tse Senkler, Jennifer Herrfurth, Cornelia Braun, Hans-Peter Feussner, Ivo Plant Physiol Focus Issue on Respiration Mitochondria are often considered as the power stations of the cell, playing critical roles in various biological processes such as cellular respiration, photosynthesis, stress responses, and programmed cell death. To maintain the structural and functional integrities of mitochondria, it is crucial to achieve a defined membrane lipid composition between different lipid classes wherein specific proportions of individual lipid species are present. Although mitochondria are capable of self-synthesizing a few lipid classes, many phospholipids are synthesized in the endoplasmic reticulum and transferred to mitochondria via membrane contact sites, as mitochondria are excluded from the vesicular transportation pathway. However, knowledge on the capability of lipid biosynthesis in mitochondria and the precise mechanism of maintaining the homeostasis of mitochondrial lipids is still scarce. Here we describe the lipidome of mitochondria isolated from Arabidopsis (Arabidopsis thaliana) leaves, including the molecular species of glycerolipids, sphingolipids, and sterols, to depict the lipid landscape of mitochondrial membranes. In addition, we define proteins involved in lipid metabolism by proteomic analysis and compare our data with mitochondria from cell cultures since they still serve as model systems. Proteins putatively localized to the membrane contact sites are proposed based on the proteomic results and online databases. Collectively, our results suggest that leaf mitochondria are capable—with the assistance of membrane contact site-localized proteins—of generating several lipid classes including phosphatidylethanolamines, cardiolipins, diacylgalactosylglycerols, and free sterols. We anticipate our work to be a foundation to further investigate the functional roles of lipids and their involvement in biochemical reactions in plant mitochondria. Oxford University Press 2023-01-24 /pmc/articles/PMC10069894/ /pubmed/36691154 http://dx.doi.org/10.1093/plphys/kiad035 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Focus Issue on Respiration
Liu, Yi-Tse
Senkler, Jennifer
Herrfurth, Cornelia
Braun, Hans-Peter
Feussner, Ivo
Defining the lipidome of Arabidopsis leaf mitochondria: Specific lipid complement and biosynthesis capacity
title Defining the lipidome of Arabidopsis leaf mitochondria: Specific lipid complement and biosynthesis capacity
title_full Defining the lipidome of Arabidopsis leaf mitochondria: Specific lipid complement and biosynthesis capacity
title_fullStr Defining the lipidome of Arabidopsis leaf mitochondria: Specific lipid complement and biosynthesis capacity
title_full_unstemmed Defining the lipidome of Arabidopsis leaf mitochondria: Specific lipid complement and biosynthesis capacity
title_short Defining the lipidome of Arabidopsis leaf mitochondria: Specific lipid complement and biosynthesis capacity
title_sort defining the lipidome of arabidopsis leaf mitochondria: specific lipid complement and biosynthesis capacity
topic Focus Issue on Respiration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10069894/
https://www.ncbi.nlm.nih.gov/pubmed/36691154
http://dx.doi.org/10.1093/plphys/kiad035
work_keys_str_mv AT liuyitse definingthelipidomeofarabidopsisleafmitochondriaspecificlipidcomplementandbiosynthesiscapacity
AT senklerjennifer definingthelipidomeofarabidopsisleafmitochondriaspecificlipidcomplementandbiosynthesiscapacity
AT herrfurthcornelia definingthelipidomeofarabidopsisleafmitochondriaspecificlipidcomplementandbiosynthesiscapacity
AT braunhanspeter definingthelipidomeofarabidopsisleafmitochondriaspecificlipidcomplementandbiosynthesiscapacity
AT feussnerivo definingthelipidomeofarabidopsisleafmitochondriaspecificlipidcomplementandbiosynthesiscapacity